A5039831639- Natural product bioactivities and synthesis
- Autophagy in Disease and Therapy
- Telomeres, Telomerase, and Senescence
- Genomics, phytochemicals, and oxidative stress
- Bioactive Compounds and Antitumor Agents
- MicroRNA in disease regulation
- Cancer Research and Treatments
- NF-κB Signaling Pathways
- Synthesis and biological activity
- Cancer Cells and Metastasis
- Advanced biosensing and bioanalysis techniques
- Carbohydrate Chemistry and Synthesis
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Phytochemicals and Antioxidant Activities
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Ginseng Biological Effects and Applications
- Advanced Breast Cancer Therapies
- Tannin, Tannase and Anticancer Activities
- Natural Compounds in Disease Treatment
- Healthcare and Venom Research
- Hepatitis B Virus Studies
Poznan University of Medical Sciences
2013-2024
Vall d'Hebron Institut de Recerca
2012
Vall d'Hebron Institute of Oncology
2012
Korea University Medical Center
2012
Human telomeres were one of the first discovered and characterized sequences forming quadruplex structures. Association these structures with oncogenic tumor suppressor proteins suggests their important role in cancer development therapy efficacy. Since cationic porphyrin TMPyP4 is known as G-quadruplex stabilizer telomerase inhibitor, aim study was to analyze anticancer properties this compound two different human breast-cancer MCF7 MDA-MB-231 cell lines. The cytotoxicity alone or...
Non-coding RNAs have been described as crucial regulators of gene expression and guards cellular homeostasis. Some recent papers focused on vault RNAs, one the classes non-coding RNA, their role in cell proliferation, tumorigenesis, apoptosis, cancer response to therapy, autophagy, which makes them potential therapy targets oncology. In human genome, four RNA paralogues can be distinguished. They are associated with complexes, considered largest ribonucleoprotein complexes. The protein part...
The lesson from many studies investigating the efficacy of targeted therapy in glioblastoma (GBM) showed that a future perspective should be focused on combining multiple target treatments. Our research aimed to assess drug combinations against stem cells (GSCs). Patient-derived U3042, U3009, and U3039 were obtained Human Glioblastoma Cell Culture resource. Additionally, study was conducted GBM commercial U251 cell line. Gene expression analysis related receptor tyrosine kinases (RTKs),...
The aim of the study was to analyze consequence silencing genes coding for key subunits telomerase complex, i.e. TERT, TERC and TP1 in human breast cancer MCF7 MDA-MB-231cells. transfection performed using Lipofectamine2000 pooled siRNAs. cytotoxic and/or antiproliferative effect siRNA measured by SRB assay, cell cycle analysed flow cytometry DNA fragmentation TUNEL analysis. Telomerase activity assessed TRAP, followed PAGE ELISA assays. downregulation also qPCR order estimate changes...
The cytotoxicity of 27 benzanilides and dithiobenzanilides built on a stilbene scaffold possessing various functional groups in aromatic rings previously described for their spasmolytic properties was assayed three human cancer cell lines (A549 –lung adenocarcinoma, MCF-7 estrogen dependent breast adenocarcinoma MDA-MB-231 independent adenocarcinoma) 2 non-tumorigenic (CCD39Lu–lung fibroblasts, MCF-12A - epithelial). Three compounds (6, 15 18) showed selective antiproliferative activity...
Telomere shortening is associated with cancer development, primarily through the induction of genomic instability. The majority studies have indicated that individuals shorter blood telomeres may be at a higher risk developing various types cancer. There increasing evidence study alterations in telomere length improve prognosis. aim present was to verify use parameters diagnostics breast stage. analyzed leukocytes 52 patients relative 47 control subjects using quantitative polymerase chain...
One of the most innovative medical trends is personalized therapy, based on simple and reproducible methods that detect unique features cancer cells. good prognostic diagnostic markers may be miRNA family. Our work aimed to evaluate changes in selected levels various breast cell lines (MCF7, MDA-MB-231, SK-BR-3) treated with doxorubicin or cisplatin. The selection was literature data regarding commonly altered miRNAs (21-3p, 21-5p, 106a-5p, 126-3p, 126-5p, 155-3p, 155-5p, 199b-3p, 199b-5p,...
Zapotin, a tetramethoxyflavone, is natural compound with wide spectrum of activities in neoplastic cells. Protein kinase C epsilon (PKCε) has been shown to be oncogenic, the ability increase cell migration, invasion and survival tumor Here we report that zapotin inhibits proliferation. In wild-type HeLa cells basal endogenous expression PKCε, IC(50) was found 17.9 ± 1.6 μM. overexpressing doxycycline-inducible constitutively active PKCε (HeLaPKCεA/E), 7.6 1.3 μM, suggesting enhances...
Breast cancer is one of the most frequently observed malignancies worldwide and represents a heterogeneous group cancers. For this reason, it crucial to properly diagnose every single case so specific efficient therapy can be adjusted. One critical diagnostic parameters evaluated in tissue status estrogen receptor (ER) epidermal growth factor (EGFR). Interestingly, expression indicated receptors may used personalized approach. Importantly, promising role phytochemicals modulation pathways...
Non-coding RNAs have been described as crucial regulators of gene expression and guards for cellular homeostasis. Some recent papers are focused on vault RNAs, one the classes non-coding RNA, their role in cell proliferation, tumorigenesis, apoptosis, cancer response to therapy, autophagy, which made them potential therapy targets oncology. In human genome, four RNA paralogues can be distinguished. They associated with complexes, considered largest ribonucleoprotein complex. The protein part...
Autophagy is engaged in tumor growth and progression, but also acts as a cell death suppression initiator. Naturally-derived compounds their derivatives constitute rich source of autophagy modulators. This paper presents the study on mechanism action oleanolic acid derivatives, HIMOXOL Br-HIMOLID, MCF7 breast cancer cells. Both reduced viability more efficiently than parental compound. It noteworthy that this effect was specific to cells, while non-cancer MCF-12A cells cytotoxicity studied...
Approximately 20-30% of the diagnosed breast cancers overexpress human epidermal growth factor receptor 2 (HER2). This type cancer is associated with a more aggressive phenotype; thus, there need for discovery new compounds that would improve survival in HER2-positive patients. It seems one most promising therapeutic strategies could be based on biological activity pentacyclic triterpenes' derivatives and best-known representative this group, oleanolic acid (OA). The its two semisynthetic...