A5062296713- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Animal Genetics and Reproduction
- Inflammasome and immune disorders
- interferon and immune responses
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Lipid Membrane Structure and Behavior
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Photodynamic Therapy Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Plant Stress Responses and Tolerance
- Porphyrin and Phthalocyanine Chemistry
- Inflammatory mediators and NSAID effects
- Neonatal Health and Biochemistry
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Neonatal and Maternal Infections
- Hypothalamic control of reproductive hormones
- Cell death mechanisms and regulation
- Insect and Pesticide Research
Norwegian University of Science and Technology
2009-2023
Central Norway Regional Health Authority
2018-2019
Centre for Inflammation Research
2014
Trondheim Kommune
2010
The Research Council of Norway
1994-1996
Abstract Lipoteichoic acid (LTA) is a central inducer of inflammatory responses caused by Gram-positive bacteria, such as Staphylococcus aureus, via activation TLR2. Localization TLR2 in relation to its coreceptors may be important for function. This study explores the signaling, uptake, and trafficking pattern LTA expression CD36 CD14 human monocytes. We found expressed early endosomes, late endosomes/lysosomes, Rab-11-positive compartments but not Golgi apparatus or endoplasmic reticulum...
Toll-like receptors (TLRs) are involved in sensing invading microbes by host innate immunity. TLR2 recognizes bacterial lipoproteins/lipopeptides, and lipopolysaccharide activates TLR4. TLR4 signal via the Toll/interleukin-1 receptor adaptors MyD88 MAL, leading to NF-κB activation. also utilizes TRAM TRIF, resulting activation of interferon regulatory factor (IRF) 3. Here, we report a new role for TRIF regulation signaling. Interestingly, observed that TLR2-mediated induction chemokine Ccl5...
Thioglucoside glucohydrolase 1 (TGG1) is one of two known functional myrosinase enzymes in Arabidopsis. The enzyme catalyzes the hydrolysis glucosinolates into compounds that are toxic to various microbes and herbivores. Transgenic Arabidopsis plants carrying beta-glucuronidase green fluorescent protein reporter genes fused 0.5 or 2.5 kb TGG1 promoter region were used study spatial activity. Promoter activity was found be highly specific restricted guard cells distinct phloem. No detected...
Signaling lymphocytic activation molecule family 1 (SLAMF1) is an Ig-like receptor and a costimulatory that initiates signal transduction networks in variety of immune cells. In this study, we report SLAMF1 required for Toll-like 4 (TLR4)-mediated induction interferon β (IFNβ) killing Gram-negative bacteria by human macrophages. We found controls trafficking the Toll receptor-associated (TRAM) from endocytic recycling compartment (ERC) to Escherichia coli phagosomes. resting macrophages,...
Phagocytosis is a complex process that eliminates microbes and performed by specialised cells such as macrophages. Toll-like receptor 4 (TLR4) expressed on the surface of macrophages recognizes Gram-negative bacteria. Moreover, TLR4 has been suggested to play role in phagocytosis bacteria, but mechanisms remain unclear. Here we have used primary human engineered THP-1 monocytes show sorting adapter, TRAM, instrumental for Escherichia coli well Staphylococcus aureus. We find TRAM forms with...
Iron is an essential nutrient for microbes, and many pathogenic bacteria depend on siderophores to obtain iron. The mammalian innate immunity protein lipocalin 2 (Lcn2; also known as neutrophil gelatinase-associated lipocalin, 24p3, or siderocalin) binds the siderophore carboxymycobactin, component of iron acquisition apparatus mycobacteria. Here we show that Lcn2 suppressed growth Mycobacterium avium in culture, M. induced production from mouse macrophages. had elevated levels initially...
Toll‐like receptor 4 ( TLR4 ) is responsible for the immediate response to Gram‐negative bacteria and signals via two main pathways by recruitment of distinct pairs adaptor proteins. Mal‐ MyD88 [Mal (MyD88‐adaptor‐like) ‐ MYD88 (Myeloid differentiation primary gene (88))] recruited plasma membrane initiate signaling cascade leading production pro‐inflammatory cytokines while TRAM‐TRIF [TRAM (TRIF‐related molecule)‐TRIF (TIR‐domain‐containing adapter‐inducing interferon‐β)] early endosomes...
Gonadotropin releasing hormone (GnRH) is responsible for stimulation of gonadotropic (GtH) in the hypothalamus-pituitary-gonadal axis (HPG). The regulatory mechanisms brain specificity make promoter attractive silico analysis and reporter gene studies zebrafish (Danio rerio).We have characterized a [Trp7, Leu8] or salmon (s) GnRH variant, gnrh3. includes 1.6 Kb upstream region displays conserved structure 4 exons 3 introns, as seen other species. An defined enhancer at -976 promoter,...
Abstract Soluble proteins that bind LPS, like myeloid differentiation-2 (MD-2) and CD14, have essential roles in regulating LPS signaling through TLR4. During a Gram-negative bacterial infection, the host may control response by adjusting levels of soluble MD-2 CD14. To address surface expression on human leukocytes, we developed mAb, IIC1, recognized both free when bound to was found freshly isolated monocytes, subpopulation CD19+ B-cells CD15+ neutrophils. transiently reduced which is most...
Single inhibition of the Toll-like receptor 4 (TLR4)-MD2 complex failed in treatment sepsis. CD14 is a coreceptor for several TLRs, including TLR4 and TLR2. The aim this study was to investigate effect single TLR4-MD2 by using eritoran, compared with alone combined C3 complement inhibitor compstatin (Cp40), on bacteria-induced inflammatory response human whole blood.Cytokines were measured multiplex technology, leukocyte activation markers CD11b CD35 flow cytometry.Lipopolysaccharide...
Infection with Shiga toxin (Stx)-producing, gram-negative bacteria can induce serious conditions such as dysentery and hemolytic uremic syndrome. In target cells, Stx is internalized by endocytosis, travels through the Golgi apparatus endoplasmic reticulum to reach cytosol, where it inhibits protein synthesis. Toll-like receptor 4 (TLR4) mediates recognition of bacteria. Here, we have investigated whether cellular uptake transport could involve TLR4. We found that upon small interfering RNA...
Inflammation plays a crucial role in the development and progression of many diseases, is often caused by dysregulation signalling from pattern recognition receptors, such as TLRs. Inhibition key protein-protein interactions an attractive target for treating inflammation. Recently, we demonstrated that lymphocyte activation molecule family 1 (SLAMF1) positively regulates downstream TLR4 identified interaction interface between SLAMF1 adaptor protein TRIF-related adapter (TRAM). Based on...
Abstract Zebrafish, Brachydanio rerio, is increasingly used in studies of early development and gene function. Major advantages are short generation time frequent production eggs which develop rapidly fresh water. Several methods for transfer have been developed zebrafish eggs, with microinjection as the most successful method. Luciferase monitoring enzyme activity live animals was investigated a tool selection stably transgenic zebrafish. Transient expression data reveals that both salmon...