A5034463009- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- HIV Research and Treatment
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 and Mental Health
- Hematopoietic Stem Cell Transplantation
- Antibiotic Resistance in Bacteria
- Acute Myeloid Leukemia Research
- Antimicrobial Resistance in Staphylococcus
- Reproductive System and Pregnancy
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Bacterial biofilms and quorum sensing
- Cell Adhesion Molecules Research
Wake Forest University
2020-2024
University of Alabama at Birmingham
2020-2022
SARS-CoV-2 causes a wide spectrum of clinical manifestations and significant mortality. Studies investigating underlying immune characteristics are needed to understand disease pathogenesis inform vaccine design. In this study, we examined cell subsets in hospitalized nonhospitalized individuals. patients, many adaptive innate cells were decreased frequency compared with those healthy convalescent individuals, the exception an increase B lymphocytes. Our findings show increased frequencies T...
Multi-drug resistant Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), has become a worldwide, major health care problem. While initially restricted to clinical settings, drug is now one of the key causative agents community-acquired infections. We have previously demonstrated that copper dependent inhibitors (CDIs), class antibiotics are only active in presence ions, effective bactericidal against MRSA. A second-generation CDI, APT-6K, exerted activity at nanomolar...
The biomolecular mechanisms controlling latent HIV-1 infection, despite their importance for the development of a cure are only partially understood. For example, ex vivo studies have recently shown that T cell activation triggered reactivation in fraction latently infected CD4+ reservoir, but molecular biology this phenomenon is unclear. We demonstrate infection primary cells and lines indeed generates substantial amount receptor (TCR)/CD3 activation-inert cells. RNA-level analysis...
Abstract Identification of early immune signatures associated with acute myeloid leukemia (AML) relapse following hematopoietic stem cell transplant (HSCT) is critical for patient outcomes. We analyzed PBMCs from 58 patients AML undergoing HSCT, focusing on T subsets and functional profiles. High-dimensional flow cytometry coupled Uniform Manifold Approximation Projection dimensionality reduction PhenoGraph clustering revealed distinct changes in CD4+ CD8+ populations 16 who relapsed within...
ABSTRACT SARS-CoV-2 causes a wide spectrum of clinical manifestations and significant mortality. Studies investigating underlying immune characteristics are needed to understand disease pathogenesis inform vaccine design. In this study, we examined cell subsets in hospitalized non-hospitalized individuals. patients, many adaptive innate cells were decreased frequency compared healthy convalescent individuals, with the exception B lymphocytes which increased. Our findings show increased...
Abstract Tetraspanins are a family of proteins with an array functions that well studied in cancer biology, but their importance immunology is underappreciated. Here we establish the tetraspanin CD151 as unique marker T-cell activation and, extension, indicator elevated, systemic activity. Baseline expression found on subset T-cells was indicative increased MAPK pathway. Following TCR/CD3 activation, upregulated overall population, quintessential feature marker. CD151+ frequencies spleen,...
HIV-1 persists in a latent reservoir memory CD4 T cells for the lifetime of patient. Understanding biomolecular mechanisms used by host to suppress viral expression will provide essential insights required develop curative therapeutic interventions.
ABSTRACT Although the ability of HIV-1 to reside in a latent state CD4+ T cells constitutes critical hurdle curative therapy, biomolecular mechanisms by which infection is established and maintained are only partially understood. E x vivo studies have shown that cell receptor/CD3 stimulation triggered reactivation fraction latently infected reservoir, suggesting parts population hosting events altered be TCR/CD3-activation-inert. We provide experimental evidence primary lines indeed...
Acute graft-versus-host-disease (GVHD), limits the use of hematopoietic cell transplant (HCT) to treat a variety malignancies. Any new therapeutic approach must satisfy three requirements: 1) Prevent GVHD, 2) Maintain anti-pathogen immunity, and 3) anti-tumor immunity. In prior studies we have shown that selective photosensitizer 2-Se-Cl eliminates highly alloreactive lymphocytes from graft HCT preventing GVHD antiviral immune responses were preserved following incubation with 2-Se-Cl. this...
Abstract We have demonstrated that the tetraspanin CD151 marks a hyperactivated, pro-inflammatory human CD4+ T cell population. Expansion of this population in HIV patients on ART may explain associated risk inflammation-related co-morbidities. However, CD151+ CD4 cells can also proliferate independent cognate antigen recognition, driven by just IL-2, feature is reminiscent latently infected which been reported to maintain latent reservoir size homeostatic proliferation mechanisms. The...
Abstract We recently reported that in humans the tetraspanin CD151 marks phenotypically distinct T cell subsets. The frequency of CD151+ cells differs between lineages and memory populations. are hyper-proliferative hyper-activated, as shown by phospho-proteomic analysis. found is an activation marker upregulated following TCR/CD3 activation, but at baseline, presence was associated with loss CD28 expression, a sign senescence. hypothesized immune hyperactivation extension, impaired...