A5102749835- Carcinogens and Genotoxicity Assessment
- Bone Metabolism and Diseases
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Bone health and treatments
- Regulation of Appetite and Obesity
- Amino Acid Enzymes and Metabolism
- Glutathione Transferases and Polymorphisms
- PI3K/AKT/mTOR signaling in cancer
- Inflammatory Bowel Disease
- Sphingolipid Metabolism and Signaling
- Neuroendocrine Tumor Research Advances
- Drug Transport and Resistance Mechanisms
- Pancreatitis Pathology and Treatment
- Acute Myeloid Leukemia Research
- Protist diversity and phylogeny
- TGF-β signaling in diseases
- Renal and related cancers
- Biochemical Analysis and Sensing Techniques
- Microbial Community Ecology and Physiology
- Helicobacter pylori-related gastroenterology studies
- Microscopic Colitis
- Bladder and Urothelial Cancer Treatments
- Kruppel-like factors research
- Cancer-related Molecular Pathways
Hamamatsu University School of Medicine
2010-2024
Gifu Pharmaceutical University
2019-2023
National Institute of Health Sciences
2019-2021
Tokyo University of Agriculture and Technology
2019-2020
Kanazawa University
2018-2019
University of the Ryukyus
1995-2019
Pharmac
2019
Osaka City University
2010-2013
Kindai University
2008
University of Michigan
1997
Bone marrow mesenchymal stem cells (MSCs) are critical regulators of postnatal bone homeostasis. Osteoporosis is characterized by volume and strength deterioration, partly due to MSC dysfunction. Cyclin-dependent kinase 8 (CDK8) belongs the transcription-related CDK family. Here, CDK8 in MSCs was identified as important for level increased aged along with association aging-related signals. Mouse genetic studies revealed that plays a crucial role resorption Mechanistically, extrinsically...
The mechanistic/mammalian target of rapamycin (mTOR) is widely implicated in the pathogenesis various diseases, including cancer, obesity, and cardiovascular disease. Bone homeostasis maintained by actions bone-resorbing osteoclasts bone-forming osteoblasts. An imbalance sophisticated regulation osteoblasts leads to as well etiology certain metabolic bone osteoporosis osteopetrosis. Here, we identified mTOR complex 1 (mTORC1) a pivotal mediator resorption under pathological conditions...
Amino acid uptake through the transporter LAT1 maintains bone homeostasis by stimulating mTORC1 signaling.
Extracellular signal-regulated kinase 5 (Erk5) belongs to the mitogen-activated protein (MAPK) family. Previously, we demonstrated that Erk5 directly phosphorylates Smad-specific E3 ubiquitin ligase 2 (Smurf2) at Thr249 (Smurf2Thr249) activate its activity. Although have clarified importance of in embryonic mesenchymal stem cells (MSCs) on skeletogenesis, role adult bone marrow (BM)-MSCs homeostasis remains unknown. Leptin receptor-positive (LepR+) BM-MSCs represent a major source and are...
Tripartite symbioses between anaerobic ciliated protists and their intracellular archaeal bacterial symbionts are not uncommon, but most reports have been based mainly on microscopic observations. Deeper insights into the function, ecology, evolution of these fascinating involving partners from all three domains life hampered by difficulties culturing ciliates in laboratory frequent loss prokaryotic during long-term cultivation. In present study, we report isolation an scuticociliate, strain...
An imbalance of branched-chain amino acids (BCAAs) in the brain may result neuropathological conditions, such as autism spectrum disorders. The L-type acid transporter 1 (LAT1), encoded by solute carrier 7a5 (Slc7a5) gene, is critical for maintaining normal levels BCAAs brain. However, our understanding mechanisms that regulate expression LAT1/Slc7a5 neurons currently limited. Here, we demonstrate hypoxic conditions upregulated Slc7a5 differentiated neuronal cells (Neuro2A induced to...
Abstract Acute kidney injury (AKI) is thought to be a reversible condition; however, growing evidence has suggested that AKI may associated with subsequent development of chronic disease. Although renal tubules have intrinsic regeneration capacity, disruption the mechanisms leads irreversible interstitial fibrosis. In this study, we investigated immunohistochemical markers in adaptive and maladaptive repair processes predict reversibility. Histopathological analysis demonstrated regenerative...
Hypothalamic neurons regulate body homeostasis by sensing and integrating changes in the levels of key hormones primary nutrients (amino acids, glucose, lipids). However, molecular mechanisms that enable hypothalamic to detect remain elusive. Here, we identified l-type amino acid transporter 1 (LAT1) leptin receptor-expressing (LepR-expressing) as being important for systemic energy bone homeostasis. We observed LAT1-dependent uptake hypothalamus, which was compromised a mouse model obesity...
Mutation of the cytochrome P450c17 (CYP17) gene causes 17 alpha-hydroxylase deficiency (17OHD). Recently, several researchers have elucidated molecular basis 17OHD by analysis. We experienced a case and intended to reveal abnormality CYP17 in this Japanese female with 17OHD. Leukocytes were obtained from patient, her mother sister, normal control subjects. amplified using polymerase chain reaction performed sequence analysis dideoxy terminator method restriction enzyme found that patient had...
o-Toluidine (o-Tol), a monocyclic aromatic amine, causes bladder cancer in humans and experimental animals is therefore classified as Group 1 carcinogen (IARC) which the carcinogenicity of o-Tol involved metabolic activation, DNA damage, adduct formation. In formation mechanism, metabolized by N-hydroxylation, N-acetoxylation, then deacetoxylation to produce an electrophilic nitrenium ion, able bind base, such dG-C8. Therefore, dG-C8-o-Tol thought be plausible exposure. However, direct...
Abstract Extracellular signal-regulated kinase 5 (Erk5), a member of the MAPK family, is specifically phosphorylated and activated by MAPK/Erk kinase-5. Although it has been implicated in odor discrimination long-term memory via its expression central nervous system, little known regarding physiological importance neuronal Erk5 body weight energy homeostasis. In current study, systemic insulin injection significantly induced phosphorylation hypothalamus. Moreover, deficiency leptin receptor...
Abstract We previously reported that immunostaining for γ‐H2AX, a biomarker of DNA damage, in the rat urinary bladder is useful early detection carcinogens 28‐day toxicity studies. Here, we aimed to examine dose dependency γ‐H2AX formation rats. Male F344 rats (aged 6 weeks) were orally administered N ‐butyl‐ ‐(4‐hydroxybutyl)nitrosamine (BBN; 0%, 0.0001%, 0.001%, 0.01%, 0.02%, or 0.05% drinking water), genotoxic carcinogen, and melamine (0%, 0.3%, 1.0%, 3.0% diet), nongenotoxic 2 days 4...
A free-living ciliate, Trimyema compressum, found in anoxic freshwater environments harbors methanogenic archaea and a bacterial symbiont named TC1 its cytoplasm. Here, we report the complete genome sequence of symbiont, consisting 1.59-Mb chromosome 35.8-kb plasmid, which was determined using PacBio RSII sequencer.
Abstract Compensation is a physiological response that occurs during chemical exposure to maintain homeostasis. Because compensatory responses are not usually considered adverse effects, it important understand mechanisms for risk assessment. Although the kidney major target organ toxicity, there controversy over whether hyperplasia or hypertrophy contributes mechanism, and limited information apply In present study, of were investigated in unilateral nephrectomy (UNx) model using adult male...
Monocyclic aromatic amines, o-toluidine (o-Tol) and its structural analog o-anisidine (o-Ans), are IARC Group 1 2A urinary bladder carcinogens, respectively, involved in metabolic activation DNA damage. Our recent study revealed that 2-methyl-N4-(2-methylphenyl) benzene-1,4-diamine (MMBD), a p-semidine-type homodimer of o-Tol, was detected identified an vitro reaction o-Tol with S9 mix vivo samples o-Tol-exposed rats. Potent mutagenic, genotoxic, cytotoxic activities were reported MMBD,...
A study of enteroaggregative Escherichia coli (EAggEC) which was recently reported as a causative agent diarrhea attempted, by isolating these organisms from the fecal samples collected sporadic patients in Miyazaki Prefecture during period January 1993 to April 1998, and investigating several characteristics isolates. By using PCR method targetting aggR gene transcriptional activator aggregative adherence fimbria I expression, thirty four strains aggR(+)-Escherichia (E. coli) were detected...
<title>Abstract</title> Background Ulcerative colitis (UC) causes extensive ulceration attributable to intestinal inflammation. This study investigated the effect of past ulcers (PEUs) on fecal calprotectin (FC). Methods retrospective, single-center, observational included patients with UC a Mayo endoscopic subscore 0. scarring or pseudopolyposis was defined as PEU and FC immunochemical occult blood test (FIT) values ere compared. The marker levels in non-PEU groups were examined assess...