A5035470185- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Synthesis and Biological Evaluation
- Genomics, phytochemicals, and oxidative stress
- Drug Transport and Resistance Mechanisms
- Nanoparticles: synthesis and applications
- Synthesis and bioactivity of alkaloids
- Air Quality and Health Impacts
- DNA and Nucleic Acid Chemistry
- Toxin Mechanisms and Immunotoxins
- PARP inhibition in cancer therapy
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Acute Lymphoblastic Leukemia research
- Plant Genetic and Mutation Studies
- Bioactive Compounds and Antitumor Agents
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Calcium signaling and nucleotide metabolism
- RNA and protein synthesis mechanisms
- Pharmacogenetics and Drug Metabolism
- Toxic Organic Pollutants Impact
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Aluminum toxicity and tolerance in plants and animals
Nihon University
2021-2024
Hoshi University
2024
Ebara (Japan)
2024
Genesis Research Institute
2015-2020
National Cancer Research Institute
2004-2020
Chemicals Evaluation and Research Institute
2015
National Cancer Centre Japan
1998-2014
Hamamatsu University School of Medicine
2012
Kyoto University
2012
In-Q-Tel
2011
Recently, manufactured nano/microparticles such as fullerenes (C60), carbon black (CB) and ceramic fiber are being widely used because of their desirable properties in industrial, medical cosmetic fields. However, there few data on these particles mammalian mutagenesis carcinogenesis. To examine genotoxic effects by C60, CB kaolin, an vitro micronuclei (MN) test was conducted with human lung cancer cell line, A549 cells. In addition, DNA damage mutations were analyzed vivo assay systems...
The genotoxic effects of multi-walled carbon nanotubes (MWCNTs) were examined by using in vitro and vivo assays. MWCNTs significantly induced micronuclei A549 cells enhanced the frequency sister chromatid exchange (SCE) CHO AA8 cells. When ICR mice intratracheally instilled with a single dose (0.05 or 0.2 mg/animal) MWCNTs, DNA damage lungs, analysed comet assay, increased dose-dependent manner. Moreover, oxidative damage, indicated 8-oxo-7,8-dihydro-2'-deoxyguanosine heptanone...
Fe3O4 magnetic nanoparticles (MgNPs-Fe3O4) are widely used in medical applications, including resonance imaging, drug delivery, and hyperthermia. However, the same properties that aid their utility clinic may potentially induce toxicity. Therefore, purpose of this study was to investigate cytotoxicity genotoxicity MgNPs-Fe3O4 A549 human lung epithelial cells. caused cell membrane damage, as assessed by release lactate dehydrogenase (LDH), only at a high concentration (100 μg/mL); lower (10...
Abstract Chronic consumption of excess ethanol increases the risk colorectal cancer. The pathogenesis ethanol-related cancer (ER-CRC) is thought to be partly mediated by gut microbes. Specifically, bacteria in colon and rectum convert acetaldehyde (AcH), which carcinogenic. However, effects chronic on human microbiome are poorly understood role microbes proposed AcH-mediated ER-CRC remains elaborated. Here we analyse compare microbiota structures non-alcoholics alcoholics. microbiotas...
Cholangiocarcinoma is a relatively rare cancer, but its incidence increasing worldwide. Although several risk factors have been suggested, the etiology and pathogenesis of majority cholangiocarcinomas remain unclear. Recently, high early-onset cholangiocarcinoma was reported among workers printing company in Osaka, Japan. These underwent exposure to organic solvents, mainly haloalkanes such as 1,2-dichloropropane (1,2-DCP) and/or dichloromethane. We performed whole-exome analysis on four...
Pierisin-1 is a potent apoptosis-inducing protein derived from the cabbage butterfly, Pieris rapae. It has been shown that pierisin-1 an A small middle dotB structure-function organization like cholera or diphtheria toxin, where "A" domain (N-terminal) exhibits ADP-ribosyltransferase activity. The present studies were designed to identify target molecule for ADP-ribosylation by in presence of beta-[adenylate-(32)P]NAD, and we found DNA as acceptor, but not case with other bacteria-derived...
The importance of ethanol oxidation by intestinal aerobes and facultative anaerobes under aerobic conditions in the pathogenesis ethanol-related colorectal cancer has been proposed. However, role obligate therein remains to be established, it is still unclear which bacterial species, if any, are most important production and/or elimination carcinogenic acetaldehyde such conditions. This study was undertaken address these issues.More than 500 strains were isolated from faeces Japanese...
One of the mutagenic and carcinogenic heterocyclic amines (HCAs), 2-amino-3,8-dimethylimidazo[4,5- f ]quinoxaline (MelQx), is present in cooked foods we are chronically exposed to this compound our daily life. To study role HCAs human carcinogenesis, analyzed MelQx-DNA adducts 38 DNA samples obtained from surgical autopsy specimens by 32 P-postlabeling method under adduct-intensification conditions with modification additional digestion nuclease P1 phosphodiesterase I after P-labeling at 5′...
Nanosized-magnetite (MGT) is widely utilized in medicinal and industrial fields; however, its toxicological properties are not well documented. In our previous report, MGT showed genotoxicity both vitro vivo assay systems, it was suggested that inflammatory responses exist behind the genotoxicity. To further clarify mechanisms underlying genotoxicity, a comprehensive DNA adduct (DNA adductome) analysis conducted using samples derived from lungs of mice exposed to MGT. total, 30 42 types...
For a long period, it has been generally considered that carcinogens, particularly genotoxic ones, have no threshold in exerting their potential for cancer induction. However, the non‐threshold theory can be challenged with regard to assessment of risk humans. Here we show food‐derived, hepatocarcinogen, 2‐amino‐3,8‐dimethylimidazo[4,5‐ f ]quinoxaline, forms DNA adducts at low doses, but does not induce glutathione S‐transferase placental form (GST‐P)‐positive foci (considered preneoplastic...
Nanomaterials are useful for their characteristic properties and commonly used in various fields. Nanosized-magnetite (MGT) is widely utilized medicinal industrial fields, whereas toxicological not well documented. A safety assessment thus urgently required MGT, genotoxicity one of the most serious concerns. In present study, we examined genotoxic effects MGT using mice revealed that DNA damage analyzed by a comet assay lungs imprinting control region (ICR) intratracheally instilled with...
We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours' treatment with particles dose-dependently increased frequency micronuclei (MN) in CHO cells up to 5.2% 5.0% at a dose 200 µg/ml (34 µg/cm²), respectively. In cells, nano-particles (2 µg/ml) for 1 hr induced H2AX phosphorylation, which is suggestive DNA double strand breaks (DSB). Treating 2 (0.34 µg/cm²) hour resulted five times higher...
Esophageal cancer is prevalent in Cixian, China, but the etiology of this disease remains largely unknown. Therefore, we explored by conducting a DNA adductome analysis. Both tumorous and nontumorous tissues were collected from patients who underwent surgical procedures at Cixian Cancer Hospital Fourth Hebei Medical University, which low-incidence area. N2-(3,4,5,6-Tetrahydro-2H-pyran-2-yl)deoxyguanosine (THP-dG) was major adduct detected samples esophageal Cixian. The precursor THP-dG,...
Norharman (9H-pyrido[3,4-b]indole), widely distributed in our environment, including cigarette smoke and cooked foodstuffs, is not mutagenic to Salmonella strains, but becomes S.typhimurium TA98 YG1024 with S9 mix the presence of non-mutagenic aromatic amines such as aniline o-toluidine. To elucidate mechanisms co-mutagenicity, we tried isolate mutagen(s) produced by a reaction between norharman mix. By HPLC purification, two compounds (I II), one (I) showing mutagenicity other (II) without...
Occupational exposure to aromatic amines (AAs) is an important risk factor for urinary bladder cancer. This study aimed evaluate the toxicity of AAs and analyze carcinogenic mechanisms in rat by comprehensive analysis DNA adducts (DNA adductome). was extracted from epithelia rats treated with AAs, including acetoacet-o-toluidine (AAOT) o-toluidine (OTD), adductome performed. Principal component analysis–discriminant revealed that OTD AAOT observed hyperplasia could be clearly separated...