A5070474104- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Infectious Diseases and Mycology
- Pneumocystis jirovecii pneumonia detection and treatment
- Parasitic Infections and Diagnostics
- Amoebic Infections and Treatments
- Mycorrhizal Fungi and Plant Interactions
- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Parasites and Host Interactions
- Mycotoxins in Agriculture and Food
- Urinary Tract Infections Management
- Antibiotic Use and Resistance
- Streptococcal Infections and Treatments
- Reproductive tract infections research
- Fungal Plant Pathogen Control
- Yeasts and Rust Fungi Studies
- Antibiotics Pharmacokinetics and Efficacy
- Mycobacterium research and diagnosis
- Complement system in diseases
- Transplantation: Methods and Outcomes
King's College Hospital
2021-2025
Imperial College London
2015-2024
Imperial College Healthcare NHS Trust
2013-2024
King's College Hospital NHS Foundation Trust
2020-2024
King's College London
2024
North West London Pathology
2019-2023
National Health Service
2013-2020
NIHR Imperial Biomedical Research Centre
2020
Charing Cross Hospital
2011-2018
St Mary's Hospital
2016
Candida auris is a globally emerging multidrug resistant fungal pathogen causing nosocomial transmission. We report an ongoing outbreak of C. in London cardio-thoracic center between April 2015 and July 2016. This the first Europe largest so far. describe identification, investigation implementation control measures.Data on case demographics, environmental screening, infection prevention/control measures, antifungal susceptibility patient isolates were prospectively recorded then analysed...
SummaryBackgroundSevere COVID-19 has a high mortality rate. Comprehensive pathological descriptions of are scarce and limited in scope. We aimed to describe the histopathological findings viral tropism patients who died severe COVID-19.MethodsIn this case series, were considered eligible if they older than 18 years, with premortem diagnosis acute respiratory syndrome coronavirus 2 infection listed clinically as direct cause death. Between March 1 April 30, 2020, full post-mortem examinations...
Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera the terminal fusarioid clade of family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept genus Fusarium was proposed. In doing so, however, significant body data supports Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA 19 orthologous protein-coding genes presented to support at F1 node Here, we demonstrate...
Candida auris was first described in 2009, and it has since caused nosocomial outbreaks, invasive infections, fungaemia across at least 19 countries on five continents. An outbreak of C. occurred a specialized cardiothoracic London hospital between April 2015 November 2016, which to date been the largest UK, involving total 72 patients. To understand genetic epidemiology infection both within this global context, we sequenced isolate genomes using Oxford Nanopore Technologies Illumina...
A rapid and global emergence of azole resistance has been observed in the pathogenic fungus Aspergillus fumigatus over past decade. The dominant mechanism appears to be environmental origin involves mutations cyp51A gene, which encodes a protein targeted by triazole antifungal drugs. Whole-genome sequencing (WGS) was performed for high-resolution single-nucleotide polymorphism (SNP) analysis 24 A. isolates, including azole-resistant susceptible clinical strains obtained from India,...
Abstract Infections caused by the fungal pathogen Aspergillus fumigatus are increasingly resistant to first-line azole antifungal drugs. However, despite its clinical importance, little is known about how susceptible patients acquire infection from drug-resistant genotypes in environment. Here, we present a population genomic analysis of 218 A. isolates across UK and Ireland (comprising 153 143 65 environmental isolates). First, phylogenomic shows strong genetic structuring into two clades...
Candida auris has caused nosocomial infections and transmissions within hospital settings. As little is known about the efficacy of skin environmental decontamination products to kill C. auris, this study investigated in vitro activity chlorine, chlorhexidine, iodine povidone vaporised hydrogen peroxide against auris. H2 O2 vapour showed 96.6%-100% effective killing All isolates were inhibited by chlorhexidine gluconate concentrations at 0.125%-1.5% for iodinated 0.07%-1.25%. Other species...
Rapid evaporative ionization mass spectrometry (REIMS) was investigated for its suitability as a general identification system bacteria and fungi. Strains of 28 clinically relevant bacterial species were analyzed in negative ion mode, corresponding data subjected to unsupervised supervised multivariate statistical analyses. The created model yielded correct cross-validation results 95.9%, 97.8%, 100% on species, genus, Gram-stain level, respectively. These not affected by the resolution...
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Members of the genus Candida, such as C. albicans and parapsilosis, are important human pathogens. Other members this genus, previously believed to carry minimal disease risk, increasingly recognised pathogens, particularly because variations in susceptibilities widely used anti-fungal agents. Thus, rapid accurate identification clinical Candida isolates is fundamental ensuring timely effective treatments delivered. Rapid Evaporative Ionisation Mass Spectrometry (REIMS) has been shown...
SUMMARY Toxoplasmosis is the fourth most common cause of hospitalization and second death due to food-borne infections. We conducted a cross-sectional study determine prevalence, disease awareness risk factors associated with toxoplasmosis among rural communities in Northern Iran. Data were obtained from serological testing participant's questionnaires analyzed using logistic regression. Of 630 participants, 465 (73.8%), 12 (1.9%) had IgG both IgM anti-Toxoplasma gondii antibodies,...
Abstract The increasing prevalence of antimicrobial resistance is a serious threat to global public health. One the most concerning trends rapid spread Carbapenemase-Producing Organisms (CPO), where colistin has become last-resort antibiotic treatment. emergence resistance, including mobilized ( mcr ) genes, raises possibility untreatable bacterial infections and motivates development improved diagnostics for detection colistin-resistant organisms. This work demonstrates response detecting...
The growing emergence of antifungal resistance has prompted the identification novel targets. G-quadruplexes (G4s), four-stranded secondary structures that form in DNA and RNA, have arisen as a drug target to treat bacterial, viral, parasitic infections. Here, we provide first demonstration G4s fungi represent promising new for development. We found PhenDC3 pyridostatin (PDS), ligands bind stabilise G4s, potently inhibited metabolism fungal pathogens different ways. These included pan...
Invasive aspergillosis (IA) is a life-threatening systemic fungal infection in immunocompromised individuals that caused by Aspergillus fumigatus. The human serum opsonin, L-ficolin, has been observed to recognize A. fumigatus and could participate defense.Using lung epithelial cells, primary monocyte-derived macrophages (MDMs), neutrophils from healthy donors, we assessed phagocytosis killing of L-ficolin-opsonized live conidia flow cytometry microscopy. Additionally, cytokines were...
Management of Candida auris infection is difficult as this yeast exhibits resistance to different classes antifungals, necessitating the development new antifungals. The aim study was investigate susceptibility C. a novel antifungal triazole, PC945, optimized for topical delivery.A collection 50 clinical isolates obtained from tertiary care hospital in North India. Nine UK, 10 CDC panel (USA) and 3 CBS-KNAW culture (Japanese South Korean isolates) were also obtained. MICs (azole endpoint)...
Summary Aspergillus fumigatus is an opportunistic fungal pathogen that typically infects the lungs of immunocompromised patients leading to a high mortality. H‐Ficolin, innate immune opsonin, produced by type II alveolar epithelial cells and could participate in lung defences against infections. Here, we used human cell line, A549, determine involvement H‐ficolin defence. Additionally, investigated presence bronchoalveolar lavage fluid from transplant during pneumonia. H‐Ficolin exhibited...
Abstract Summary The increase of antifungal drug resistance is a major global human health concern and threatens agriculture food security; in order to tackle these concerns, it important understand the mechanisms that cause resistance. curated Mycology Antifungal Resistance Database (MARDy) web-service mechanisms, including amino acid substitutions, tandem repeat sequences genome ploidy. MARDy implemented on Linux, Apache, MySQL PHP web development platform includes local installation...
Carbapenemase-producing Enterobacterales (CPE) are challenging in healthcare, with resistance to multiple classes of antibiotics. This study describes the emergence IMP-encoding CPE amongst diverse species between 2016 and 2019 across a London regional network.