Background and Aims: Intrathecal bupivacaine is used for anaesthesia analgesia but associated with hypotension. Ropivacaine an alternative drug that may have fewer cardiotoxic neurotoxic events. This meta-analysis investigated whether intrathecal ropivacaine reduced hypotension as compared to bupivacaine. Methods: The registered in the International Prospective Register of Systematic Reviews (PROSPERO). databases PubMed, Cinahl Plus, Google Scholar, Scopus were searched, papers from January...

Abstract Background Mapping protein primary sequences to their three dimensional folds referred as the 'second genetic code' remains an unsolved scientific problem. A crucial part of problem concerns geometrical specificity in side chain association leading densely packed cores, a hallmark correctly folded native structures. Thus, any model packing within proteins should constitute indispensable component folding and design. Results In this study attempt has been made find, characterize...

Plants can withstand many abiotic stresses. Stress adaptation through retention of imprints previous stress exposure has also been described in plants. We have characterized the imprint or memory adaptive responses rice seedlings to arsenic (As) and heat stress.Two-week-old (both with without As) were given a 45 °C shock for 3 h. While under shock, leafy portion was harvested at regular intervals. Subsequently, kept room temperature recovery sampling continued over Total RNA protein...

Background Treatment failure and resistance to the commonly used drugs remains a major obstacle for successful chemotherapy against visceral leishmaniasis (VL). Since development of novel therapeutics involves exorbitant costs, effectiveness currently available antitrypanosomatid drug suramin has been investigated as an antileishmanial, specifically VL,in vitro in animal model experiments. Methodology/Principal Leishmania donovani promastigotes were treated with studies performed determine...

Earlier attempts to obtain technetium complexes with cysteine always resulted in the formation of a product contaminated polymeric species. A pure product, which could be chemically characterized and adopted for radiopharmaceutical preparation, has now been obtained by using cystine as precursor cysteine. This method extended prepare corresponding rhenium chelate, isolated tetraphenylphosphonium salt [Ph(4)P](+)[{ReO(Cys)(2)}(-){HReO(Cys)(2)}].4H(2)O. The X-ray crystal structure this...

The rapid emergence of drug resistant Leishmanial strains makes it imperative to continue the development cheap and effective drugs against parasite. Due absence vaccines leishmaniasis, current therapeutic measures exclusively rely on chemotherapy. Here we attempt, identify novel antileishmanial from a list known determined previous bioinformatics study. Synergism between various combinations (involving netilmicin, suramin, paromomycin curcumin) have been estimated potent multidrug therapies...

Incorporation of ω-amino acids into peptide sequences plays an important role in designing peptides with modified backbone conformation and enhanced stability against proteolysis. The present study establishes the presence unusual turns involving 12-membered hydrogen bonded rings terminally blocked tri- tetrapeptides. X-ray diffraction analysis single crystals NMR studies have been used to probe three-dimensional structures two protected short peptides, Boc-γ-Abu(1)-Aib(2)-Ala(3)-OMe 1...

Self-assembled hydrogels by virtue of their unique 3D network and tunability have extensively been explored for bio-medical applications like tissue engineering, delivery release therapeutic agents, etc. Herein, we demonstrate the first-time nucleoside-based biocompatible with a remarkable leishmanicidal effect against both Leishmania major promastigotes amastigotes no cytotoxic on macrophage cell line. In this work, series synthesized silver ion-driven self-assembly natural nucleoside...

Drug development against Leishmania donovani, the pathogen that causes visceral leishmaniasis in humans, is currently an active area of research given widespread prevalence disease and emergence resistant strains. The immunosuppressive drug cyclosporin known to have antiparasitic activity a variety pathogens. receptor for protein cyclophilin, which ubiquitous peptidylprolyl isomerase. crystal structure cyclophilin from L. donovani complexed with has been solved at 2.6 A resolution....

The majority of the effects cyclosporin A (CsA) on cells is caused by inhibition phosphatase activity calcineurin (CN) cyclophilin (CyPA)-CsA complex formed in cytoplasm. Although CsA inhibits proliferation a large number parasites, not all are susceptible. presence structurally altered CyPA with lower affinity for had been suggested to be cause resistance. We report here identification and cloning high CsA-binding protein (LdCyP) from Leishmania donovani, trypanosomatid parasite that...

According to the "jigsaw puzzle" model of protein folding, isomorphism between sequence and structure is substantially determined by specific geometry side-chain interactions, within interior. In this work, we have attempted predict hydrophobic core cyclophilin (LdCyp) from Leishmania donovani, utilizing a surface complementarity function, which selects for high goodness fit surfaces, rather in manner assembling three-dimensional jigsaw puzzle. The computational prediction method implemented...

Sickle red blood cells display an abnormal propensity to adhere cultured bovine aortic endothelial when compared normal cells. The adherence was potentiated three‐fold by cell derived conditioned medium, enriched in multimers of von Willebrand factor. Such ablated 80% either the synthetic peptide (RGDS) or antibody GPIIb/IIIa, indicating presence RGD recognition domain/receptor sickle both. also inhibited 70% phosphatidylserine, but not other phospholipids, putative receptors for this...

Leishmaniasis,a broad spectrum of diseases caused by several sister species protozoa belonging to family trypanosomatidae and genus leishmania , generally affects poorer sections the populace in third world countries. With emergence strains resistant traditional therapies high cost second line drugs which have severe side effects, it becomes imperative continue search for alternative combat disease. In this work, leishmanial genomes human genome been compared identify proteins unique...

The crystal structure of cyclophilin from Leishmania donovani (LdCyp) has been determined and refined at 1.97 Å resolution to a crystallographic R factor 0.178 (Rfree = 0.197). was solved by molecular replacement using Trypanosoma cruzi as the search model. LdCyp exhibits complete structural conservation cyclosporin-binding site with respect homologous human protein, anticipated LdCyp–cyclosporin binding studies. Comparisons other cyclophilins show deviations primarily in loop regions....