A5057019569- Glioma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Pancreatic and Hepatic Oncology Research
- Thyroid Cancer Diagnosis and Treatment
- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- Radiomics and Machine Learning in Medical Imaging
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Pancreatitis Pathology and Treatment
- Genetic factors in colorectal cancer
- Meningioma and schwannoma management
- Neuroendocrine Tumor Research Advances
- Brain Metastases and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Amyotrophic Lateral Sclerosis Research
- Pituitary Gland Disorders and Treatments
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Neurogenetic and Muscular Disorders Research
- Ovarian cancer diagnosis and treatment
- COVID-19 Clinical Research Studies
- Head and Neck Anomalies
University of Bologna
2014-2023
Azienda USL di Bologna
2015-2022
Policlinico S.Orsola-Malpighi
2018-2022
Creative Research Enterprises (United States)
2021-2022
Ospedale Bellaria
2011-2019
Ospedale Maggiore
2016
Istituto Oncologico Romagnolo
2012
Several biomarkers have been proposed as useful parameters to better specify the prognosis or delineate new target therapy strategies for glioblastoma patients. MicroRNAs could represent putative molecules, considering their role in tumorigenesis, cancer progression and specific tissue expression. Although several studies tried identify microRNA signature glioblastoma, a profile is still far from being well‐defined. In this work expression of 19 microRNAs (miR‐7, miR‐9, miR‐9∗, miR‐10a,...
Small papillary thyroid carcinomas have contributed to the worldwide increased incidence of differentiated cancer observed over past decades. However, mortality rate has not changed same period time, raising questions about possibility that patients, especially those with small tumors, are overdiagnosed and overtreated. Molecular prognostic marker able discriminate aggressive cancers from an indolent course would be great relevance tailor therapeutic approach reduce overtreatment. Mutations...
Selection of lung cancer patients for therapy with tyrosine kinase inhibitors directed at EGFR requires the identification specific mutations. In most advanced, inoperable carcinoma limited tumor samples often represent only material available both histologic typing and molecular analysis. We defined a next generation sequencing protocol targeted to exons 18-21 suitable routine diagnosis such clinical samples. The was validated in an unselected series 80 small biopsies (n=14) cytology (n=66)...
The use of endoscopic ultrasonography has allowed for improved detection and pathologic analysis fine needle aspirate material pancreatic lesion diagnosis. molecular KRAS further the clinical sensitivity preoperative analysis. For this reason, highly analytical sensitive specific tests in from specimens become great importance. In present study, 60 were analyzed exon 2 3 mutations, using three different techniques: Sanger sequencing, allele locked nucleic acid PCR Next Generation sequencing...
The homogeneous distribution of BRAF V600E in papillary thyroid carcinoma (PTC) has been called into question by recent reports. These studies claim that is heterogeneous and limited to tumor cell subsets the majority PTCs.The objective study was understand allele evaluating percentage mutated neoplastic cells a group PTCs using two different highly sensitive analytical approaches: allele-specific locked nucleic acid PCR 454 next-generation sequencing targeted exon 15.BRAF investigated on...
Next generation sequencing (NGS) allows parallel of multiple genes at a very high depth coverage. The need to analyze variety targets for diagnostic/prognostic/predictive purposes requires multi-gene characterization. Multi-gene panels are becoming standard approaches the molecular analysis solid lesions. We report custom-designed 128 panel engineered cover relevant in 22 oncogene/oncosuppressor tumors most frequently subjected routine genotyping. A total 1695 were analyzed validation....
The use of tyrosine kinase inhibitors (TKIs) requires the testing for hot spot mutations molecular effectors downstream membrane-bound kinases since their wild type status is expected response to TKI therapy. We report a novel assay that we have called Allele Specific Locked Nucleic Acid quantitative PCR (ASLNAqPCR). uses LNA-modified allele specific primers and beacon probes increase sensitivity, specificity accurately quantify mutations. designed codon 12/13 KRAS BRAF V600E, validated with...
BACKGROUND: Pancreatic adenocarcinoma (PDAC) is one of the deadliest human malignancies. Although surgery currently only effective treatment for PDAC, most patients survive less than 20 months after tumor resection. OBJECTIVE: The primary goal was to investigate alterations in KRAS, TP53, SM AD4 and CDKN2A/p16 tumors from with exceptionally long survival surgery. METHODS: Tumors 15 PDAC that survived more 55 ("LS") were analyzed IDH1, NRAS BRAF using next-generation sequencing. SMAD4 tested...
BRAF alterations, namely fusion and V600E mutation, have been recently reported in low-grade epilepsy-associated tumors. Twenty tumors were retrieved to evaluate the mutational status. mutations present 10 concomitantly associated dysplastic tissue of three patients. We here show for first time that are not only but, some cases, also focal cortical dysplasia.
Background: Non-canonical mutations of the isocitrate dehydrogenase (IDH) genes have been described in about 20–25% and 5–12% patients with WHO grade II III gliomas, respectively. To date, prognostic value these rare is still a topic debate. Methods: We selected gliomas IDH1 available tissue samples for next-generation sequencing. The clinical outcomes baseline behaviors canonical R132H non-canonical were compared. Results: evaluated 433 harboring mutations. Three hundred ninety (90.1%) had...
miRNAs are small molecules involved in gene regulation. Each tissue shows a characteristic epression profile that could be altered during neoplastic transformation. Glioblastoma is the most aggressive brain tumour of adult with high rate mortality. Recognizing specific pattern for GBM provide further boost target therapy. The availability fresh specimens often limited and this reason possibility starting from formalin fixed paraffin embedded (FFPE) very helpful even expression analysis. We...
Glioblastoma is the most aggressive brain tumor that may occur in adults. Regardless of huge improvements surgery and molecular therapy, outcome neoplasia remains poor. MicroRNAs are small molecules involved several cellular processes, their expression altered vast majority tumors. Several studies reported different miRNAs glioblastoma, but one critical point understanding glioblastoma profile comparison these studies. In this paper, we focused our attention on non-neoplastic references used...
// Greta Gandolfi 1 , Moira Ragazzi 2 Dario de Biase 3 Michela Visani 4 Eleonora Zanetti Federica Torricelli Valentina Sancisi Mila Gugnoni Gloria Manzotti Luca Braglia 5 Silvio Cavuto Domenico Franco Merlo Giovanni Tallini Andrea Frasoldati 6 Simonetta Piana and Alessia Ciarrocchi Laboratory of Translational Research, Azienda Unità Sanitaria Locale di Reggio Emilia - IRCCS, 42123, Italy Pathology Unit, Department Oncology, Pharmacology Biotechnology (FaBiT), University Bologna, 40139...
In cystic and solid pancreatic lesions, KRAS mutational status refines the diagnosis of uncertain endoscopic ultrasound (EUS) aspirates. This test should have a fast turnaround time ideally be performed at centre where patient is diagnosed. The Idylla Mutation Test enables standardisation even in units without molecular expertise.
Epidermal Growth Factor Receptor (EGFR) molecular analysis is performed to assess the responsiveness Tyrosine Kinase Inhibitors (TKIs) in patients with Non-Small Cell Lung Cancer (NSCLC). The existence of intra-tumoral heterogeneity has been observed lung cancers. aim present study investigate if percentage mutated neoplastic cells within tumor sample might influence TKIs treatment.A total 931 cases NSCLC were analyzed for EGFR mutational status (exon 18, 19, 20, 21) using Next Generation...
Context.— Integration of the analysis genetic markers with endoscopic ultrasound–guided fine-needle aspiration and cytologic evaluation has increased accuracy preoperative diagnosis pancreatic lesions. The application high-throughput gene panel using next-generation sequencing platforms is now offering a great opportunity for further improvements. Objective.— To review to Data Sources.— For data acquisition, PubMed search terms sequencing, pancreas, lesions, tumors, EUS-FNA was performed...
Advanced medullary thyroid cancers (MTCs) are now being treated with drugs that inhibit receptor tyrosine kinases, many of which involved in angiogenesis. Response rates vary widely, and toxic effects common, so treatment should be reserved for MTCs likely to responsive these drugs. RET mutations common MTCs, but it is unclear how they influence the microvascularization tumors. We examined 45 germ-line or somatic (RETmut group) 34 wild-type (RETwt). Taqman Low-Density Arrays were used assess...