A5055714436- Cancer Genomics and Diagnostics
- Thyroid Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Cervical Cancer and HPV Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Sarcoma Diagnosis and Treatment
- Pancreatic and Hepatic Oncology Research
- BRCA gene mutations in cancer
- Ovarian cancer diagnosis and treatment
- Bladder and Urothelial Cancer Treatments
- Epigenetics and DNA Methylation
- Endometrial and Cervical Cancer Treatments
- Cutaneous Melanoma Detection and Management
- Chromatin Remodeling and Cancer
- Colorectal and Anal Carcinomas
- Neuroendocrine Tumor Research Advances
- Renal and related cancers
- Genetic factors in colorectal cancer
- Cancer Mechanisms and Therapy
- Colorectal Cancer Treatments and Studies
- Renal cell carcinoma treatment
- Gallbladder and Bile Duct Disorders
- Genetic and rare skin diseases.
- Cancer Immunotherapy and Biomarkers
Azienda USL di Bologna
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2022-2025
University of Bologna
2020-2024
Creative Research Enterprises (United States)
2021-2022
Policlinico S.Orsola-Malpighi
2022
Next generation sequencing (NGS) allows parallel of multiple genes at a very high depth coverage. The need to analyze variety targets for diagnostic/prognostic/predictive purposes requires multi-gene characterization. Multi-gene panels are becoming standard approaches the molecular analysis solid lesions. We report custom-designed 128 panel engineered cover relevant in 22 oncogene/oncosuppressor tumors most frequently subjected routine genotyping. A total 1695 were analyzed validation....
Purpose: The prognostic importance of RET and RAS mutations their relationship to clinicopathologic parameters outcomes in medullary thyroid carcinoma (MTC) need be clarified.
The European Society of Gynecologic Oncology/European Radiation Therapy and Pathology (ESGO/ESTRO/ESP) committee recently proposed a new risk stratification system for endometrial carcinoma (EC) patients that incorporates clinicopathologic molecular features. aim the study is to compare ESGO/ESTRO/ESP classification with previous 2016 recommendations, evaluating impact defining algorithm selecting cases analysis assign appropriate class.The cohort included 211 consecutive EC patients....
High-grade serous ovarian carcinoma (HGSOC) is one of the deadliest malignancies in female population and cause 70% all cancer-related deaths. Among its hallmarks, fluid accumulation peritoneal cavity, or ascites, a peculiar pathological sign during late stages recurrent patients. Besides cancer cells, ascitic fluids contain heterogeneous cellular composition, representing precious source to dissect molecular mechanisms underlying invasion metastatization find new biomarkers predict therapy...
Introduction Endometrial endometrioid carcinomas can show multiple lines of differentiation, including pilomatrix-like high-grade carcinoma, a recently described tumor with similarity to cutaneous pilomatrix carcinoma and associated very aggressive clinical behavior. Methods We present 56-year-old woman an endometrial secondary involvement both ovaries, left tubo-ovarian ligament obturator lymph nodes. The diagnosis in previously performed curettage was confirmed the hysterectomy specimen....
To analyze the diagnostic accuracy and feasibility of digital-PCR (dPCR) combined with next-generation sequencing (NGS) in ERCP-guided histological diagnosis biliary strictures to overcome issue represented by scarcity sampled material. Twenty-two prospective patients were included, submitted ERPC-guided biopsy or resection. By histopathological analysis plus fluorescence situ hybridization (FISH) for chromosomes 3, 7, 17 aneuploidies, 8 cases (36.4%) malignant, 14 (63.6%) negative. NGS was...
Aims Mucosal melanoma of the head and neck (MM–H&N) is an aggressive disease known for its frequent residual tumours/relapses (RT/R) at surgical site, as well eventual metastases. Our understanding MM–H&N mutational landscape, together with correlation specific mutations clinical–pathological features, significantly less comprehensive compared to that cutaneous melanoma. Additionally, status consecutive samples collected from single patients has not been investigated, which limits...
Despite the efforts made in management of PDAC, 5-year relative survival rate pancreatic ductal adenocarcinoma (PDAC) still remains very low (10%). To date, precision oncology is far from being ready to be applied cases although studies exploring molecular and genetic alterations have been conducted, genomic landscape PDAC has characterized. This study aimed apply a next-generation sequencing (NGS) laboratory-developed multigene panel samples find that could associated with histopathological...
The BRAF p.V600E mutation represents the most specific marker for papillary thyroid carcinoma and is potentially related to aggressive behavior persistent disease. alterations other than are less common in represent an alternative mechanism of activation with unclear clinical significance. study aims describe frequency clinicopathologic characteristics non-V600E mutations a large cohort (1654 samples) lesions characterized by next-generation sequencing. have been found 20.3% (337/1654)...
Cutaneous melanoma is one of the most lethal tumors among skin cancers, characterized by complex genetic and molecular alterations that result in uncontrolled cell proliferation metastatic spread. Next-generation sequencing (NGS) enables simultaneous examination numerous genes, making this technique essential for diagnosis, prognostic stratification, therapy planning. Herein, we present experience with our laboratory-designed NGS panel routine assessment advanced-stage melanoma. A total 260...
The molecular characterization of endometrial carcinoma (EC) has recently been included in the ESGO/ESTRO/ESP guidelines. study aims to evaluate impact integrated and pathologic risk stratification clinical practice relevance parameters predicting prognosis each EC subgroup. ECs were classified using immunohistochemistry next-generation sequencing into four classes: POLE mutant (POLE), mismatch repair deficient (MMRd), p53 (p53abn), no specific profile (NSMP). According WHO algorithm, 219...
Since the Cancer Genome Atlas (TCGA) project identified four distinct groups based on molecular alterations, mutation analyses have been integrated into characterization of endometrial carcinomas (ECs). ARID1A seems to be subunit more involved in loss function SWI/SNF complex ECs. The aim this study is define relevance alterations a cohort EC, studying possible associations between DNA (genomic level), RNA expression (transcriptomic and protein (proteomic level). A total 50 were...
•RAI resistance and time to RAI-R development in advanced DTC are significantly correlated with genetic profiling.•Genetic testing allows for identifying the subset of more aggressive those actionable gene fusions.•Genetic should be included as part clinical workup carried out early possible all DTCs. BackgroundTumor genotyping is becoming crucial optimize management patients differentiated thyroid cancer (DTC); however, its implementation practice remains undefined. We herein report our...
Abstract Surgical resection for hepatocellular carcinoma (HCC) is burdened with a high recurrence rate and lack of reliable prognostic factors. The aim this study was to integrate the HCC pathological features gene mutations improve role analysis. This monocentric prospective study, including 67 patients resected HCC. All clinical data histological were collected, tumor grade, architecture, margins, microvascular invasion, microscopic portal vascular invasion (MPVI). Next-generation...
Abstract Canine liposarcoma is an uncommon tumor that shares morphological similarities with its human counterpart. In dogs, the genetic features of this are unknown and, based on immunohistochemical studies, amplification gene MDM2 and mutation TP53 suspected. study 51 cases primary liposarcomas were immunohistochemically stained for p53 subjected to fluorescent in situ hybridization next-generation sequencing detect mutations, respectively. expressed 21 6 cases, mutations identified 10 15...