Abstract N6-methyladenosine (m 6 A) serves as the most common and conserved internal transcriptional modification. However, roles of m A on cervical cancer (CC) tumorigenesis are still unclear. Here, results indicated that METTL3 was significantly upregulated in CC tissue cells, which closely correlated with lymph node metastasis poor prognosis patients. MeRIP-Seq analysis revealed profiles cells. Functionally, promoted proliferation Warburg effect (aerobic glycolysis) Mechanistically,...

In light of carbon-neutral pledge, the oil and gas industry has been facing several critical new challenges in China. The current status terms market mechanism reform, supply-consumption balance key technology innovation China's are reviewed present study, strategies roadmaps proposed to cope with challenges. study found that (i) faces such as incomplete pricing mechanisms, unclear subject rights, lack recognition trading carbon assets. (ii) trade-off between short-term supply security...

Abstract Background There is growing evidence discussing the role of long non-coding RNAs (lncRNAs) in cervical cancer (CC). We performed this study to explore impact exosomal lncRNA urothelial cancer-associated 1 (UCA1) CC stem cells by sponging microRNA-122-5p (miR-122-5p) and regulating SOX2 expression. Methods (CD133 + CaSki) exosomes were extracted identified. The synthesized UCA1- miR-122-5p-related sequences transfected into CaSki cells, cells-derived then co-cultured with CD133...

Pre-diabetes and type 2 diabetes mellitus (T2DM) are important stages of metabolic disorders, in which abnormal lipid metabolism plays a key role. In recent years, the development lipidomics technology has provided new perspective to reveal differences plasma composition between pre-diabetes T2DM patients. This paper discusses changes profile patients with prediabetes based on lipidomics, looks forward future research directions, order provide ideas for early intervention treatment

The outgrowth and metastasis of cervical cancer (CC) contribute to its malignancy. Pituitary Tumor Transforming Gene 1 (PTTG1) is upregulated in many types cancer, enhances tumor cell growth metastasis. However, the activation regulation PTTG1 CC, especially by pseudogene PTTG3P, have not been shown. Here, we detected significantly higher levels PTTG3P resected CC tissue, compared paired adjacent normal tissue. Interestingly, positively correlated with levels. High were associated poor...

Abstract Background The efficacy of preoperative biliary drainage (PBD) has been debated for several decades, and yet indications PBD remain controversial. aim this study was to compare the postoperative morbidity mortality in patients with malignant obstructive jaundice undergoing direct surgery versus PBD. Methods All consecutive who underwent radical resection between June 2017 December 2019 at Zhongshan Hospital were analyzed retrospectively. population divided into two groups: group...

Glucagon-like peptide-1 (GLP-1) has considerable potential as a possible therapeutic agent for type-2 diabetes. Unfortunately, this glucoincretin is short lived due to degradation by dipeptidyl-peptidase IV and rapid clearance renal filtration. In study, we attempted extend GLP-1 action through the attachment of lysine residue at N-terminal (named KGLP-1), make fusion protein with human serum albumin (HSA) in Pichia pastoris. The protein, designated KGLP-1/HSA, was purified an immunomagnetic...

Kidney‑type glutaminase (GLS1) plays a significant role in tumor metabolism. Our recent studies demonstrated that GLS1 was aberrantly expressed hepatocellular carcinoma (HCC) and facilitated progression. However, the roles of intrahepatic cholangiocarcinoma (ICC) remain largely unknown. Thus, aim this study to evaluate expression clinical significance ICC. For purpose, combined data from Oncomine database with those immunohistochemistry were used determine levels cancerous non‑cancerous...

This study aimed to investigate the molecular mechanisms underlying roles of metformin (MET) and Sorafenib (SOR) in treatment endometrial hyperplasia (EH) polycystic ovary syndrome (PCOS). Effects MET SOR on area endometrium myometrium were detected. Western blot analysis immunohistochemistry assays carried out detect levels mammalian target rapamycin complex 1 (mTORC1), mTORC2, LC3-II, P62, Caspase-3 rats cultured cells. Furthermore, cell counting kit-8 assay flow cytometry was determine...

The MUC16 C-terminal (MUC16c) level is associated with tumor serum CA-125 levels, however, the roles remain unclear in gallbladder carcinoma (GBC). In this study, we found that MUC16c promoted glucose uptake and glycolysis for GBC cell proliferation. Mass spectrometry analysis suggested could combine aldolase. ALDOC mRNA protein are overexpressed tumors. IHC results also showed consistent up-regulation of. tissues than peritumor tissues. We determined combining stability disrupted ability of...

Abstract Both total alkaloids from Feculae Bombycis (TAFB) and natural flavonoids can inhibit α-glucosidase activity to depress the glucose level in blood. To investigate cooperative effect of TAFB on blood glucose, we have studied their combined function compared with individual ingredients enzymology, in-vitro in-vivo. In enzymological assay, combination showed more effective inhibition, either or alone, activity. everted intestine model in-vitro, inhibition starch hydrolysation...

Understanding how cancer cells regulate endocytosis during the cell cycle could lead us to capitalize this event pharmacologically. Although certain pathways are attenuated mitosis, shift and regulation have not been well clarified. The conventional concept of glucose-regulated proteins (GRPs) as protein folding chaperones was updated by discoveries that translocated GRPs assume moonlighting functions modify immune response, viral release, control intracellular trafficking. In study, GRP75,...

Background: Successful implementation of gene therapy heavily relies on efficiently delivering genetic materials and specific targeting into cells. Oncogene-driven endocytosis stimulates nutrient uptake also develops an endocytosis-mediated defense against therapeutic agents. Cell-penetrating peptides, typically HIV-Tat, are well known for efficient delivery nucleic acid drugs but lack specificity. Various passive strategies were pursued to enhance the tumor efficiency; however, they still...

Abstract Background The distant metastasis (DM) mode and treatment efficacies in the advanced biliary tract cancer (BTC) were obscure, a credible evaluation is urgently needed. Method A total of 6348 BTC patients (ICC, intrahepatic cholangiocarcinoma, n = 1762; PHCC, perihilar 1103; GBC, gallbladder cancer, 2580; DCC, distal 538; AVC, carcinoma Vater ampulla, 365) enrolled from Surveillance, Epidemiology, End Results (SEER) database. Propensity score matching (PSM) process was carried out...

Abstract Chaperone-assisted proteasome degradation of oncogenic protein acts as an upstream signal controlling tumorigenesis and progression. The understanding the co-regulation chaperone oncoprotein endocytosis pathways is extremely limited. In this study, we showed for first time that proto-Dbl ( dbl proto-oncogene product) co-enriched with mitochondrial GRP75 in vesicles from ovarian cancer cells. onco-Dbl, produced by mutation/degradation proto-Dbl, markedly enhanced cellular...

To explore the effects of microRNA (miR)-27b-3p-mediated homeobox A10 (HOXA10) on proliferation, migration, and invasion endometriosis cells (hEM15A).First, quantitative polymerase chain reaction (qPCR) was performed for measurement miR-27b-3p HOXA10 expression in hEM15A human embryonic stem (hESC). Then, targeted relationship with verified by conducting a dual-luciferase reporter experiment. Subsequently, qPCR western blot were to determine effect expression. Finally, Cell Counting Kit-8,...

Introduction Ovarian cancer (OVCA) is one of the most prevalent malignant tumors female reproductive system, and its diagnosis typically accompanied by production ascites. Although liquid biopsy has been widely implemented recently, or prognosis OVCA based on remains primary emphasis. Methods In this study, using proximity barcoding assay, a technique for analyzing surface proteins single extracellular vesicles (EVs). For validation, serum ascites samples from patients with epithelial...