A5028446840- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Air Quality and Health Impacts
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Energy, Environment, Economic Growth
- Virus-based gene therapy research
- Energy, Environment, and Transportation Policies
- CRISPR and Genetic Engineering
- HIV/AIDS Research and Interventions
- Cell Adhesion Molecules Research
- Air Quality Monitoring and Forecasting
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Global Health Care Issues
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related molecular mechanisms research
- Peripheral Neuropathies and Disorders
- Peptidase Inhibition and Analysis
- Nanowire Synthesis and Applications
Weill Cornell Medicine
2025
Memorial Sloan Kettering Cancer Center
2025
Cornell University
2025
Wenzhou Medical University
2024
Yale University
2022-2024
Systems Biology Institute
2024
Zhejiang Lab
2024
Fujian Medical University
2023
Yale New Haven Health System
2022-2023
Yale Cancer Center
2023
Abstract The Omicron variant of SARS-CoV-2 recently swept the globe and showed high level immune evasion. Here, we generate an Omicron-specific lipid nanoparticle (LNP) mRNA vaccine candidate, test its activity in animals, both alone as a heterologous booster to WT vaccine. Our LNP-mRNA elicits strong antibody response vaccination-naïve mice. Mice that received two-dose show > 40-fold reduction neutralization potency against than two weeks post boost, which further reduce background after...
A major limiting factor in the success of chimeric antigen receptor (CAR) T cell therapy for treatment solid tumors is targeting tumor antigens also found on normal tissues. CAR cells against GD2 induced rapid, fatal neurotoxicity because recognition + mouse brain tissue. To improve selectivity cell, we engineered a synthetic Notch that selectively expresses upon binding to P-selectin, adhesion protein overexpressed neovasculature. These microenvironment actuated (MEAT) ameliorated...
Lipid nanoparticle (LNP)-mRNA vaccines offer protection against COVID-19; however, multiple variant lineages caused widespread breakthrough infections. Here, we generate LNP-mRNAs specifically encoding wild-type (WT), B.1.351, and B.1.617 SARS-CoV-2 spikes, systematically study their immune responses. All three induced potent antibody T cell responses in animal models; differences neutralization activity have been observed between variants. vivo challenges of authentic viruses WA-1, Beta,...
Although COVID-19 vaccines have been developed, multiple pathogenic coronavirus species exist, urging on development of multispecies vaccines. Here we develop prototype lipid nanoparticle (LNP)-mRNA vaccine candidates against SARS-CoV-2 Delta, SARS-CoV, and MERS-CoV, test how multiplexing LNP-mRNAs can induce effective immune responses in animal models. Triplex duplex LNP-mRNA vaccinations antigen-specific antibody SARS-CoV-2, MERS-CoV. Single-cell RNA sequencing profiles the global systemic...
Phosphatase and tensin homolog deleted on chromosome ten (PTEN) serves as a powerful tumor suppressor, has been found to be downregulated in human bladder cancer (BC) tissues. Despite this observation, the mechanisms contributing PTEN's downregulation have remained elusive.
Bladder cancer (BC) is among the most prevalent malignant urothelial tumors globally, yet prognosis for patients with muscle-invasive bladder (MIBC) remains dismal, a very poor 5-year survival rate. Consequently, identifying more effective and less toxic chemotherapeutic alternatives critical enhancing clinical outcomes BC patients. Isorhapontigenin (ISO), novel stilbene isolated from Gnetum found in certain provinces of China, has shown potential as an anticancer agent due to its diverse...
Natural killer (NK) cells are an innate immune cell type that serves at the first level of defense against pathogens and cancer. NK have clinical potential, however, multiple current limitations exist naturally hinder successful implementation therapy cancer, including their effector function, persistence, tumor infiltration. To unbiasedly reveal functional genetic landscape underlying critical characteristics we perform perturbomics mapping infiltrating by joint in vivo AAV-CRISPR screens...
The Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has high transmissibility and recently swept the globe. Due to extensive number mutations, this level immune evasion, which drastically reduced efficacy existing antibodies vaccines. Thus, it is important test an Omicron-specific vaccine, evaluate its response against other variants, compare immunogenicity as boosters with vaccine designed reference wildtype virus (WT). Here, we generated lipid nanoparticle...
Abstract A 44-year-old man who presented with progressive right limb weakness was diagnosed ischemic stroke. He referred for 18 F-DPA-714 PET/CT evaluation of the disease. showed increased uptake intracranial thrombus. This DPA-714–avid thrombus highly suggested involvement immune cells in extension clot resulting neurological deterioration. present case that PET might be a promising tracer visualizing thromboinflammation vivo.
Current clinical detection of Human immunodeficiency virus 1 (HIV-1) is used to target viral genes and proteins. However, the immunoassay, such as culture or Polymerase Chain Reaction (PCR), lacks accuracy in diagnosis, these conventional assays rely on stable genome HIV-1 a highly-mutated virus. Next generation sequencing (NGS) promises be transformative for practice infectious disease, rapidly reducing cost processing time mean that this will become feasible technology diagnostic research...
Abstract The Omicron sub-lineage BA.2 of SARS-CoV-2 has recently become dominant across many areas in the world on-going waves COVID-19. Compared to ancestral/wild-type (WT) virus, lineage variants, both BA.1 and BA.2, contain high number mutations, especially spike protein, causing significant immune escape that leads substantial reduction vaccine antibody efficacy. Because this antigenic drift, exhibited differential resistance profile monoclonal antibodies than BA.1. Thus, it is important...
Abstract Adoptive cell therapy has shown clinical success in patients with hematological malignancies. Immune engineering is critical for production, research, and development of therapy; however, current approaches generation therapeutic immune cells face various limitations. Here, we establish a composite gene delivery system the highly efficient cells. This system, termed MAJESTIC ( m RNA A AV-Sleeping-Beauty J oint E ngineering S table T herapeutic I mmune C ells), combines merits mRNA,...
Abstract Radiotherapy (RT), is a fundamental treatment for malignant tumors and used in over half of cancer patients. As radiation can promote anti-tumor immune effects, promising therapeutic strategy to combine with checkpoint inhibitors (ICIs). However, the genetic determinants that impact response context combination therapy ICI have not been systematically investigated. To unbiasedly identify tumor intrinsic factors governing such responses, we perform set genome-scale CRISPR screens...
Abstract The Omicron variant (B.1.1.529) of SARS-CoV-2 rapidly becomes dominant globally. Its extensive mutations confer severe efficacy reduction to most existing antibodies or vaccines. Here, we developed RAMIHM , a highly efficient strategy generate fully human monoclonal (mAbs), directly applied it with Omicron-mRNA immunization, and isolated three potent specific clones against Omicron. Rapid mRNA immunization elicited strong anti-Omicron antibody response in humanized mice, along...
Ground-level ozone is a continuing problem worldwide, but there insufficient research about the influence of pollution on labor productivity in developing countries. Couriers are important for e-commerce and rapidly express delivery industry around world. Yet, due to their typical outdoor working environments, they susceptible health effects pollution. We investigate effect worker service sector using unique panel dataset courier from top-five company China. Using an instrumental variable...
Abstract Although successful COVID-19 vaccines have been developed, multiple pathogenic coronavirus species exist, urging for development of multi-species vaccines. Here we developed prototype LNP-mRNA vaccine candidates against SARS-CoV-2 (Delta variant), SARS-CoV and MERS-CoV, test how multiplexing these LNP-mRNAs can induce effective immune responses in animal models. A triplex scheme vaccination induced antigen-specific antibody SARS-CoV-2, with a relatively weaker MERS-CoV response this...
In recent years, with a gradual increase of health awareness, society has become more concerned about the frequent occurrence air pollution. As is known to all, fine particulate matter (particles less than 2.5 micrometers in diameter [PM2.5]) main cause haze, which suspended and generated through series physical chemical changes. Of note, different components sources PM2.5 kinds damage. Identification analysis have guiding significance for prevention Indoor that mainly from smoking biomass...