A5034908898- Microscopic Colitis
- Celiac Disease Research and Management
- Inflammatory Bowel Disease
- Immune Cell Function and Interaction
- Bioinformatics and Genomic Networks
- Digestive system and related health
- Helicobacter pylori-related gastroenterology studies
- IL-33, ST2, and ILC Pathways
- Gut microbiota and health
- Eosinophilic Esophagitis
- Gastrointestinal motility and disorders
- Immune cells in cancer
- Gastrointestinal disorders and treatments
- Hidradenitis Suppurativa and Treatments
- T-cell and B-cell Immunology
- Cancer Cells and Metastasis
- Immune Response and Inflammation
- Viral Infections and Immunology Research
- Immunodeficiency and Autoimmune Disorders
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Whipple's Disease and Interleukins
- Genetic factors in colorectal cancer
Mútua Terrassa
2018-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2018-2025
Instituto de Salud Carlos III
2021-2025
Universitat de Barcelona
2020-2025
University Hospital Mútua de Terrassa
2018-2020
Centro de Investigación Biomédica en Red
2020
Although macrophages (Mϕ) maintain intestinal immune homoeostasis, there is not much available information about their subset composition, phenotype and function in the human setting. Human Mϕ (CD45+HLA-DR+CD14+CD64+) can be divided into subsets based on expression of CD11c, CCR2 CX3CR1. Monocyte-like cells identified as CD11chighCCR2+CX3CR1+ cells, a also shared by circulating CD14+ monocytes. On contrary, Mϕ-like tissue-resident counterparts display CD11c-CCR2-CX3CR1- phenotype. CD11chigh...
Butyrate-producing gut bacteria are reduced in patients with active inflammatory bowel disease (IBD), supporting the hypothesis that butyrate supplementation may be beneficial this setting. Nonetheless, earlier studies suggest oxidation of IBD is altered. We propose inflammation decrease epithelial consumption.Non-IBD controls and were recruited for study. Stool samples used short-chain fatty acid bacterial butyryl CoA:acetate CoA-transferase quantification. Colonic biopsies ex vivo...
Diagnosing celiac disease (CD) in individuals adhering to a gluten-free diet (GFD) presents significant challenges. Current guidelines recommend gluten challenge (GC) lasting at least 6–8 weeks, which has several limitations. Our aim was compare four approaches previously proposed for diagnosing CD on GFD: IL-2 serum levels, gut-homing CD8+ T cells, % TCRγδ+ intraepithelial lymphocytes (IELs), and UBE2L3 gene expression. Additionally, we evaluated the T-cell-based method with 3-day GC...
The upper oesophagogastrointestinal (UEGI) tract histology, intestinal morphometry and lymphocyte subpopulations of healthy people is scarcely known. In research studies inflammation involving the UEGI tract, there a lack adequate controls. Aims: To evaluate histology duodenal volunteers patients with gastroesophageal reflux disease (GERD), latter to assess if it could replace subjects. Healthy individuals were excluded they had symptoms, comorbidities, pregnancy, toxics, medications or...
The differential diagnosis between patients with celiac disease (CD) and non-celiac gluten sensitivity (NCGS) is difficult when a gluten-free diet (GFD) has been initiated before the diagnostic work-up. Isolated increases in TCRγδ+ lymphogram (increased plus decreased CD3-) may enable this challenging clinical setting. This study evaluated: (1) accuracy of %TCRγδ+ for diagnosing CD after GFD differentiation NCGS; (2) kinetics at baseline starting both NCGS.
Summary Background The causes of seronegative villous atrophy can be grouped as coeliac or noncoeliac related. There is no consensus on how to approach subjects with disease. Aim To evaluate the accuracy both an increase in CD3 + T‐cell receptor gamma delta (TCRγδ ) intraepithelial lymphocytes and lymphogram for diagnosis disease patients atrophy. Methods Sixty‐seven consecutive were included. Duodenal biopsies assess TCRγδ − by flow cytometry performed at index endoscopy. Coeliac was...
Vedolizumab is an anti-α4β7 antibody approved for the treatment of ulcerative colitis [UC]. Although it assumed that vedolizumab blocks intestinal homing lymphocytes, its effects on different cell populations are not fully stablished. In order to establish unique mechanisms action in UC patients, we compared those induced by anti-tumour necrosis factor [TNF].Patients with active [endoscopic Mayo score >1] starting [n = 33] or anti-TNF 45] and controls 22] were included. Colon biopsies [at...
Background Diagnosing celiac disease (CD) in individuals adhering to a gluten-free diet (GFD) presents significant challenges. Current guidelines recommend gluten challenge (GC) lasting at least 6-8 weeks, which has several limitations. Objectives This study compares four approaches previously proposed for diagnosing CD on GFD: IL-2 serum levels, gut-homing CD8 + T cells, %TCRγδ intraepithelial lymphocytes (IELs), and UBE2L3 gene expression. Additionally, we evaluated the T-cell based method...
Introduction: The study of intraepithelial lymphocytes (IEL) by flow cytometry is a useful tool in the diagnosis coeliac disease (CD). Previous data showed that an increase %TCRγδ+ and decrease %CD3− IEL constitute typical CD cytometric pattern with specificity 100%. However, there are no regarding whether differences related to sex, age, titers serology, degree histological lesion. Study aims: To confirm high diagnostic accuracy patterns. determine if between serology titers, lesion grade....
An on-site, rapid, fingertip, whole-blood point-of-care test (POCT) is attractive for active case-finding of coeliac disease (CD) in primary care because its simplicity.The aim this article to assess the usefulness and cost-effectiveness adult using a POCT based on deamidated gliadin peptide antibodies (IgA/IgG-DGP) CD diagnosis.A study was conducted by an easy-to-use, IgA/IgG-DGP-based fingertip compared with tTG2 350 individuals. Sample size calculated 0.28% prevalence reference...
Background Personalized medicine requires finding relationships between variables that influence a patient’s phenotype and predicting an outcome. Sparse generalized canonical correlation analysis identifies different groups of variables. This method establishing model the expected interaction those Describing these interactions is challenging when relationship unknown or there no pre-established hypothesis. Thus, our aim was to develop find microbiome host transcriptome data relevant...
Abstract Background The diagnosis of coeliac disease (CD) in individuals that have started a gluten-free diet (GFD) without an adequate previous diagnostic work-out is challenge. Several immunological assays such as IFN-γ ELISPOT been developed to avoid the need prolonged gluten challenge induce intestinal damage. We aimed evaluate accuracy activated gut-homing CD8 + and TCRγδ T cells blood after 3-day compare it with performance HLA-DQ2.5 subsample. Methods A total 22 CD patients 48 non-CD...
1. Background: The long-term effect of a gluten-free diet (GFD) on functional bowel disorders (FBDs) has been scarcely studied. aim was to assess the GFD FBD patients, and role both low-grade coeliac score lymphogram in probability response GFD. 2. Methods: 116 adult patients with either predominant diarrhoea or abdominal bloating, fulfilling Rome IV criteria FBD, were treated Duodenum biopsies performed for pathology studies intraepithelial lymphocyte subpopulation patterns. Coeliac defined...
Background & Aims: Determining whether patients with lymphocytic enteritis (LE) have coeliac disease is a challenge. We analysed the variables associated low-grade enteropathy diagnosis in suspected but without villous atrophy, and developed scoring system to identify them. Methods: collected data from 2010 through 2016 on persistent symptoms compatible clinical spectrum of disease. One hundred four starting gluten-free diet (GFD) were included. Duodenal biopsies before GFD for numbers...
Previous studies suggested that Interleukin-10 (IL-10) depletion in Crohn's disease (CD) could predict outcome. To determine IL-10 blood and at different intestinal locations patients with active CD to assess its potential prognostic capacity identify aggressive CD. Twenty-three were included. Ulcerative colitis (UC), infectious healthy individuals acted as controls. Serum mucosal samples taken baseline 1 month after steroid initiation patients. Patients classified according response....
Inflammatory bowel disease (IBD), including Crohn's (CD) and ulcerative colitis (UC), is thought to be driven by an exacerbated immune response the commensal microbiota. Macrophages (MΦ) are most abundant mononuclear phagocytes in gastrointestinal tract, where they critical at shaping type of elicited towards However, there not much available information about human MΦ subset composition, phenotype function tract. Here, we aimed characterise intestinal health patients with inflammatory (IBD)...
Crohn’s disease (CD) is a chronic inflammatory bowel (IBD) with onset occurring from childhood to late age. Despite comparable genetic susceptibility, phenotype and natural history vary between paediatric adult-onset CD. Recent studies have highlighted the importance of intestinal epithelial barrier in pathogenesis IBD [1] [2] [3]. We hypothesise that epithelium patients CD characterised by age-dependent differences gene expression signature. Biopsy samples ileum colon adult were collected....
Vedolizumab (VDZ) was approved for IBD treatment in 2014. It targets the integrin ɑ4β7, which facilitates migration of leucocytes to intestine. VDZ achieves clinical remission <45% ulcerative colitis (UC) patients, and currently there are no predictors response guide decisions. Our aim unravel mechanism action underlying by assessing its effect UC patients how they correlate with response/remission VDZ. In this regard, our specific objectives were: (1) analyse frequencies leukocytes...
Abstract Background Vδ1+ and Vδ2+ are TCRγδ lymphocytes of the gut intraepithelial compartment that recognise proteins without help antigen-presenting cells. Its relevance in inflammatory bowel disease (IBD) is unknown. Aim To measure T-cell subtypes [CD4+, CD8+, double positive (DP,CD4+CD8+), negative (DN,CD4−CD8−) CD103+] healthy IBD inflammed mucosa. Methods Twenty-six active patients immunosuppressants (n = 18 Crohn’s (CD), eight ulcerative colitis (UC)) 10 controls (paired biopsies...
Abstract Background CD161 is a type C lectin expressed in NKs cells and peripheral T (TCRγδ αβ, NKTs), enriched intestinal populations. Its expression can be modulated by infections inflammation. MAIT are subset of innate antimicrobial T-cells abundant the mucosa but their role immunological regulation still unknown. Aim To measure levels subtypes T-lymphocytes mucosa: CD4+, CD8+, double positive (DP,CD4+CD8+), negative (DN,CD4−CD8−), (CD161+TCRVα7.2+) intraepithelial (CD103+) Methods...
Abstract Background Personalized medicine requires finding relationships between variables that influence a patient’s phenotype and predicting an outcome. Sparse generalized canonical correlation analysis identifies different groups of variables. This method establishing model the expected interaction those Describing these interactions is challenging when relationship unknown or there no pre-established hypothesis. Aim To develop to find microbiome transcriptome data relevant clinical in...