A5058725590- Psoriasis: Treatment and Pathogenesis
- Immunotherapy and Immune Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Dendrimers and Hyperbranched Polymers
- T-cell and B-cell Immunology
- Ferrocene Chemistry and Applications
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Angiogenesis and VEGF in Cancer
- HER2/EGFR in Cancer Research
- Multiple Sclerosis Research Studies
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Antiplatelet Therapy and Cardiovascular Diseases
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Platelet Disorders and Treatments
- Retinal Diseases and Treatments
- RNA Interference and Gene Delivery
- S100 Proteins and Annexins
Medical University of Lodz
2011-2023
Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) in which activated T cell and neutrophil interactions lead to neuroinflammation. In this study the expression CCR6, CXCR2, CXCR6 Th17 cells neutrophils migrating brain during EAE was measured, alongside evaluation production IL-17, IL-23, CCL-20, CXCL16 brain. Next, inflammatory subpopulations accumulating after intracerebral injections IL-17 or CXCL1, as well modulation with anti-IL-23R anti-CXCR2...
The nature of the interaction between Th17 cells and blood-brain barrier (BBB) is critical for development autoimmune inflammation in central nervous system (CNS). Tumor necrosis factor alpha (TNF-α) or interleukin 17 (IL-17) stimulation known to enhance adherence brain endothelium. endothelial (bEnd.3) express Vascular cell adhesion molecule 1 (VCAM-1), receptor responsible inflammatory adhesion, which binds very late antigen 4 (VLA-4) on migrating effector lymphocytes at early stage...
Abstract Purpose To examine the involvement of F11R/JAM-A protein in breast cancer metastasis, we utilized antagonistic peptide 4D (P4D) experiments transendothelial migration (TEM) cells. Methods Experiments were conducted mouse 4T1 model utilizing human mammary epithelial cell and endothelial lines. The levels soluble (sJAM-A) murine plasmas measured by ELISA. Levels mRNA lines assessed qRT-PCR Western blot, respectively. Cell surface expression was demonstrated flow cytometry. Functional...
Aging has become a significant risk factor for several diseases, including breast cancer.Platelet activation and platelet-cancer cell aggregate fractions were found to increase with tumor progression in mouse model of cancer. At advanced stages development, platelets from mice cancer hyperreactive low agonist concentrations hyporeactive high ones. Platelet reactivity strongly associated metastasis the lungs extramedullary hematopoiesis liver. A greater fraction platelet aggregates was...
The F11R/JAM-A cell adhesion protein was examined as the therapeutic target in triple negative breast cancer (TNBC) with use of peptide antagonist to F11R/JAM-A, that previously inhibited early stages metastasis vitro.The online silico analysis performed by TNMPlot, UALCAN, and KM plotter. vitro experiments were verify effect 4D (P4D) on human endothelial lines EA.hy926 HMEC-1 well TNBC line MDA-MB-231. morphology upon P4D treatment verified light microscopy, while functions assessed colony...
Platelet-derived protein disulfide isomerase 1 (PDIA1) regulates thrombus formation, but its role in the regulation of platelet function is not fully understood.The aim this study was to characterize PDIA1 human platelets.Proteomic analysis PDI isoforms platelets performed using liquid chromatography tandem mass spectometry, and expression PDIs on response collagen, TRAP-14, or ADP measured with flow cytometry. The effects bepristat, a selective inhibitor, aggregation, surface activation...
Carbosilane metallodendrimers, based on the arene Ru(II) complex (CRD13) and integrated to imino-pyridine surface groups have been investigated as an anticancer agent in a mouse model with triple-negative breast cancer. The dendrimer entered into cells efficiently, exhibited selective toxicity for 4T1 cells. In vivo investigations proved that local injection of CRD13 caused reduction tumour mass was non-toxic. ICP analyses indicated accumulated all tested tissues greater content detected tumour.
Several studies report elevated blood platelet activation and altered count in COVID-19 patients, but the role of SARS-CoV-2 spike protein this process remains intriguing. Additionally, there is no data that anti-SARS-CoV-2 neutralizing antibodies (nAb) may attenuate activity toward platelets. Our results indicate under vitro conditions, increased collagen-stimulated aggregation isolated platelets induced binding vWF to ristocetin-treated blood. The also significantly reduced collagen- or...
Blood platelets’ adenosine receptors (AR) are considered to be a new target for the anti-platelet therapy. This idea is based on in vitro studies which show that signaling mediated by these leads decreased platelet response activating stimuli. In vivo evidence antithrombotic activity of AR agonists published date were limited, however, usage relatively high doses given bolus. The present study was aimed at verifying if substances used lower combination with inhibitors P2Y12 could serve as...
The nature of the interaction between Th17 cells and blood-brain barrier (BBB) is critical for development autoimmune inflammation in central nervous system (CNS). TNF-a or IL-17 stimulation known to enhance adherence brain endothelium. endothelial (bEnd.3) express VCAM-1, receptor responsible inflammatory cell adhesion, which binds VLA-4 on migrating effector lymphocytes at early stage inflammation. present study examines effect pro-inflammatory cytokines bEnd.3 were found increase...