A5001001895- Olfactory and Sensory Function Studies
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
- Food composition and properties
- Advanced Chemical Sensor Technologies
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Growth Hormone and Insulin-like Growth Factors
- Protein Tyrosine Phosphatases
- Phytase and its Applications
- Food Quality and Safety Studies
- Potato Plant Research
- Probiotics and Fermented Foods
- Phytoestrogen effects and research
- Endoplasmic Reticulum Stress and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Environmental DNA in Biodiversity Studies
- Microbial Inactivation Methods
- Cardiac electrophysiology and arrhythmias
- Identification and Quantification in Food
- T-cell and Retrovirus Studies
- Cancer Treatment and Pharmacology
- Balance, Gait, and Falls Prevention
Asahikawa Medical University
2010-2024
Tokyo University of Agriculture
2014-2024
Hiroshima University
2021
Imakiire General Hospital
2019-2020
Chiba University
2007
McGill University
2005-2006
Kagoshima University
2003-2005
Kyushu Institute of Technology
1996-2003
Oita University
2003
Kobe University
1994-2000
SH-PTP2 is a nontransmembrane human protein-tyrosine phosphatase that contains two Src homology 2 (SH2) domains and binds to insulin receptor substrate 1 (IRS-1) via these in response insulin. The expression of catalytically inactive mutant (containing the mutation Cys-459-->Ser) Chinese hamster ovary cells overexpress receptors (CHO-IR cells) markedly attenuated insulin-stimulated Ras activation. Expression also inhibited MAP kinase activation but not 12-O-tetradecanoyl...
PTPlC is a non-transmembrane protein-tyrosine phosphatase and contains two Src homology-2 (SH2) domains.Insulin stimulated the tyrosine phosphorylation of in human 1M-9 lymphoblast cells, rat H35 hepatoma cells Chinese hamster ovary overexpressing both insulin receptors PTPlC.Insulin also mutant lacking SIB domains suggesting that SH2 are not required for insulin-stimulated PTPlC.The receptor kinase catalyzed cell-free system.Peptide mapping phosphorylated showed Ws8 C-terminal region was...
The subventricular zone (SVZ) generates an immense number of neurons even during adulthood. These migrate to the olfactory bulb (OB) and differentiate into granule cells periglomerular cells. information broadcast by general odorants is received sensory transmitted OB. Recent studies have shown that a reduction mastication impairs both neurogenesis in hippocampus brain functions. To examine these effects, we first measured difference Fos-immunoreactivity (Fos-ir) at principal trigeminal...
Mutations of a putative cyclic-nucleotide-binding domain (CNBD) can disrupt the function hyperpolarization-activated cyclic-nucleotide-gated channel (HCN2) and human ether-a-go-go-related gene potassium (HERG). Loss caused by C-terminal truncation, which includes all or part CNBD in HCN HERG, has been related to abnormal trafficking. Similar defects have reported for several missense mutations HERG associated with long QT syndrome type 2 (LQT2). Thus, we postulate that normal processing...
Abstract Background A previous Phase I/II study demonstrated that TAS‐102 (trifluridine/tipiracil [FTD/TPI]) plus bevacizumab (Bev) has encouraging efficacy and controllable safety for patients with previously treated metastatic colorectal cancer. Therefore, we designed assessing the of FTD/TPI Bev in elderly untreated Methods This is a multicenter, single‐arm II included ≥70 years old untreated, unresectable Treatment consisted given every 4 weeks. The primary endpoint was progression‐free...
Exposure to environmental neurotoxins is suspected be a risk factor for sporadic progressive neurodegenerative diseases. Parkinson's disease has been associated with exposure the pesticide rotenone, mitochondrial respiration inhibitor. We previously reported that intranasal administration of rotenone in mice induced dopaminergic (DA) neurodegeneration olfactory bulb (OB) and reduced functions. In present study, we investigated DA neurons brains were administered intranasally an extended...
In this study, the relationship between dissociation of κ-casein from casein micelles due to heat-induced denaturation and strength acid milk gel was investigated. The κ-casein-dissociated were fractionated by filtration chromatography two-dimensional polyacrylamide electrophoresis, their zeta potential surface hydrophobicity measured. negative charge lower than that native micelles, micellar higher. For confirmation, isoelectric point found cohere at an earlier stage acidification micelles....
We recently demonstrated that (±)-(Z)-2-(aminomethyl)-1-phenyl-N,N-diethylcyclopropanecarboxamide [milnacipran, (±)-1], an inhibitor of the reuptake serotonin (5-HT), was a noncompetitive NMDA receptor antagonist. On basis cyclopropane structure (±)-1, conformationally restricted analogs with different stereochemistries, namely 1-phenyl-2-(1-aminoalkyl)-N,N-diethylcyclopropanecarboxamindes (2, 3, ent-2, and ent-3), were designed synthesized. Among these analogs, 2a, 2b, 2f,...
The Kv4.2 transient voltage-dependent potassium current contributes to the morphology of cardiac action potential as well neuronal excitability and firing frequency. Here we report profound effects C terminus on surface expression activation gating properties that are modulated by direct interaction between KChIP2, an auxiliary regulatory subunit, Kv4.2. We show increasingly large truncations rat (wild type) cause a progressive decrease along with shift in is closely correlated negative...
Bipedal gait involves moving the body while maintaining an upright posture under gravity. Throughout vertebrate evolution and postnatal development, humans acquired antigravity functions that allow one to achieve biped gait. While walking, our attention is focused on purposeful, intentional movements such as dexterous arm-hand finger or searching for target. On other hand, postural control comes awareness only when we need alter patterns, facing demanding conditions. Nonetheless, brain so...
We investigated the blocking effect of conformationally restricted analogs milnacipran on NMDA receptors by recording whole-cell currents Xenopus oocytes injected with rat brain mRNA and single channel cultured hippocampal neurons under voltage-clamp conditions. Their protective against excitotoxicity was also cortex neurons. All examined blocked activated receptors, though their structures were quite different from known receptor blockers. The a (1S, 2R, 1'S)-configuration such as PPDC...
Agosterol A (AG-A) is a novel agent that reverses P-glycoprotein (P-gp) and multidrug resistance protein-1 (MRP1)-meditated (MDR). We have synthesized [125I]11-azidophenyl agosterol (azidoAG-A), photoaffinity analog of AG-A, characterized its binding to P-gp in membrane vesicles prepared from multidrug-resistant P-gp-overexpressing KB-C2 cells. The photoanalog photolabeled intact both the N- C-terminal fragments P-gp. [125I]AzidoAG-A transported by intracellular accumulation [125I]azidoAG-A...
Cremophor EL (CrEL) is a non-ionic surfactant widely used as vehicle for insoluble drugs, including immunosuppressive and anticancer agents. Although CrEL has often been reported to induce sensory neuropathies, its action on voltage-gated ion channels remains unknown. We show here that modulates sodium current (INa) potassium (IK) of human neuroblastoma cells (SH-SY5Y). First, suppressed the amplitude INa IK. The suppression–concentration curve was gradual but IK steeper, indicating...
The binding of insulin to its receptor rapidly induces intrinsic tyrosine kinase activity, resulting in phosphorylation various cytosolic substrates, such as substrate-1 (IRS-1) which, turn, associates with a p85 subunit phosphatidylinositol 3-kinase (PI 3-kinase) followed by activation this enzyme. In the present study, we have examined these early steps signalling rat liver vivo after food ingestion. After fasting for 22 h, 12% casein diet was available ad libitum throughout 8-h...
The effect of storage at 0°C and 10°C on physical biochemical parameters 'Danshaku' tubers was monitored to provide baseline information this cultivar's behavior. Sugar starch level, weight loss, firmness, specific gravity, tissue color, fatty acid composition phospholipicls, sprouting respiration rate, content, particle size, pasting properties were monitored. accumulation occurred in stored 0°C, while minimal sugar severe loss observed for 10°C. Tubers consistently softer due loss. No...
TRPV2, a member of the transient receptor potential family, has been isolated as capsaicin-receptor homolog and is thought to respond noxious heat. Here we show that TRPV2 mRNA predominantly expressed in subpopulation olfactory sensory neurons (OSNs). We carried out histochemical analyses insulin-like growth factor-I (IGF-IR) using situ hybridization immunofluorescence adult system. In mucosa, intensive immunostaining was observed at axon bundles but not soma. TRPV2-positive labeling...