A5102006770- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Ferrocene Chemistry and Applications
- Respiratory viral infections research
- Click Chemistry and Applications
- Metal complexes synthesis and properties
- Epigenetics and DNA Methylation
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Mesenchymal stem cell research
- Synthesis and Biological Evaluation
- Inflammation biomarkers and pathways
- Nanoplatforms for cancer theranostics
- Pneumonia and Respiratory Infections
- Immune cells in cancer
- Neuroinflammation and Neurodegeneration Mechanisms
Nanjing University
2013-2024
Fudan University
2018-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2022
Huashan Hospital
2022
Shanghai Medical College of Fudan University
2016-2018
Academia Sinica
2011
Academy of Medical Sciences
2011
University of Calgary
2010-2011
Peking Union Medical College Hospital
2011
East China Normal University
2008
We recently demonstrated the utility of quantifying spontaneous pain in mice via blinded coding facial expressions. As majority preclinical research is fact performed laboratory rat, we attempted to modify scale for use this species. present herein Rat Grimace Scale, and show its reliability, accuracy, ability quantify time course intraplantar complete Freund's adjuvant, intraarticular kaolin-carrageenan, laparotomy (post-operative pain) assays. The scale's demonstrate dose-dependent...
Adoptive transfer of tumor infiltrating or circulating lymphocytes transduced with antigen receptors has been examined in various clinical trials to treat human cancers. The antigens targeted by transferred affects the efficacy this therapeutic approach. Because cancer stem cells (CSCs) play an important role growth and metastasis, we hypothesized that adoptive T targeting a CSC could result dramatic anti-tumor effects. An EpCAM-specific chimeric receptor (CAR) was constructed transduce...
Platinum(<sc>iv</sc>) prodrugs targeting the DNA repair mechanism downregulate myeloid cell leukemia-1 (Mcl-1) and homologous recombination proteins (RAD51, BRCA2), thereby enhancing cytotoxicity against cisplatin-resistant cancer cells.
Abstract Triggering receptor expressed on myeloid cells‐2 (TREM2) is a key pro‐tumorigenic marker of tumor‐infiltrating macrophages, showing potent immunosuppressive activity in tumor microenvironment. A platinum(IV) complex OPA derived from oxaliplatin (OP) and artesunate (ART) exhibited direct cytotoxicity against human colon cancer cells immunomodulatory to inhibit TREM2 macrophages vitro vivo. Furthermore, deterred the growth mouse models bearing MC38 colorectal by reducing number CD206...
Activating the cyclic GMP-AMP synthase-stimulator of interferon gene (cGAS-STING) pathway is a promising immunotherapeutic strategy for cancer treatment. Manganese(ii) complexes MnPC and MnPVA (P = 1,10-phenanthroline, C chlorine, VA valproic acid) were found to activate cGAS-STING pathway. The not only damaged DNA, but also inhibited histone deacetylases (HDACs) poly adenosine diphosphate-ribose polymerase (PARP) impede repair DNA damage, thereby promoting leakage fragments into cytoplasm....
Interplay between breast cancer (BC) cells and the tumor microenvironment (TME) influences outcome of treatment. Aberrant activation signal transducer activator transcription 3 (STAT3) promotes interaction causes immunosuppression drug resistance. Platinum(IV) complexes SPP DPP bearing pterostilbene-derived axial ligand(s) were synthesized to inhibit JAK2-STAT3 pathway in BC regulate TME. These exerted remarkable antiproliferative activity against triple-negative cells, suppressed expression...
Recently, the immunostimulatory roles of chemotherapeutics have been increasingly revealed, although bone marrow suppression is still a common toxicity chemotherapy. While numbers and ratios different immune subpopulations are analyzed after chemotherapy, changes to status each cycle treatment less studied remain unclear. To determine tumor-specific functions cycles we treated CT26 tumor-bearing mice with one four 5-fluorouracil (5-FU). Overall survival was not improved when more than 5-FU...
Most of the anticancer compounds synthesized by chemists are primarily evaluated for their direct cytotoxic effects at cellular level, often overlooking critical role immune system. In this study, we developed a patient-derived, T-cell-retaining tumor organoid model that allows us to evaluate efficacy chemical drugs under synergistic paradigm antigen-specific T-cell-dependent killing, which may reveal missed drug hits in simple assay. We clinically approved platinum (Pt) and custom library...
// Wen Zhang 1, * , Li Bao 3, 9, 10, Shaoxing Yang 6, Zhaoyang Qian Mei Dong 4, Liyuan Yin 2, 19 Zhao 7 Keli Ge 1 Zhenling Deng Jing 5 Fei Qi 8 Zhongxue An 3 Yuan Yu Qingbo Wang Renhua Wu Fan Lianfeng 16 Xiping Chen 17 Yingjian Na Lin Feng 2 Lingling liu Yujie Zhu Tiancheng Qin Shuren Youhui Xiuqing 11, 12 Jian 13 Xin Yi Liqun Zou Hong-Wu 18 Henrik J. Ditzel 14 Hongjun Gao 6 Kaitai Binlei Liu 15, Shujun Cheng Department of Immunology, Cancer Hospital, Chinese Academy Medical Sciences and...
Mitochondrial DNA (mtDNA) is an attractive cellular target for anticancer agents in addition to nuclear (nDNA). The cationic platinum(II) complex cis-[Pt(NP)(NH3)2Cl]NO3 (PtNP, NP = N-(2-ethylpyridine)-1,8-naphthalimide) bearing the DNA-intercalating moiety was designed. structure of PtNP fully characterized by single-crystal X-ray crystallography, NMR, and HRMS. superior cisplatin both vitro vivo activities with low systemic toxicity. interaction CT-DNA demonstrated that could effectively...
Assessment of infection with helminth parasites in murine models disease could identify antiinflammatory mechanisms that translate into treatments for arthritic disease. The aim this study was to test the ability tapeworm Hymenolepis diminuta ameliorate Freund's complete adjuvant (CFA)-induced monoarthritis mice.Mice received CFA or without H diminuta, and knee swelling, pain, measures inflammation were assessed.Injection resulted rapid (within 24 hours) sustained (lasting 20 days) a...
Abstract Background The primary objective of this study was to test whether oncolytic herpes simplex virus type 1 (HSV1) could eradicate chemoresistant cancer stem cells (CSCs). Methods fluorescent aldefluor reagent-based technique used identify and isolate ALDH br as CSCs from the 4T1 murine breast cell line. presence also examined in transplanted immune-competent syngeneic mice. Results Compared with lo cells, had a markedly higher ability form tumor spheres vitro tumorigenic potential...
Oncolytic viruses are promising treatments for many kinds of solid tumors. In this study, we constructed a novel oncolytic herpes simplex virus type 2: oHSV2. We investigated the cytopathic effects oHSV2 in vitro and tested its antitumor efficacy 4T1 breast cancer model. compared effect on cell cycle immunologic impact with traditional chemotherapeutic agent doxorubicin. data showed that infected most human murine tumor lines was highly oncolytic. killed cells independent their phase,...
Abstract Respiratory syncytial virus (RSV) infection can cause severe disease in the lower respiratory tract of infants and older people. Vaccination with a formalin-inactivated RSV vaccine (FI-RSV) subsequent has led to mild pneumonia two deaths among vaccinees. The vaccine-enhanced (VED) was recently demonstrated be due an elevated level Th2 cell responses following loss regulatory T (Treg) cells from lungs. To induce high levels neutralizing Abs minimize pathogenic responses, we developed...
A Pt<sup>IV</sup> prodrug bearing a vascular disrupting agent demonstrates antiangiogenic and antimetastatic potentialities against metastatic triple-negative breast cancer cells.
Abstract Most of the anticancer compounds synthesized by chemists are primarily evaluated for their direct cytotoxic effects at cellular level, often overlooking critical role immune system. In this study, we developed a patient‐derived, T‐cell‐retaining tumor organoid model that allows us to evaluate efficacy chemical drugs under synergistic paradigm antigen‐specific T‐cell‐dependent killing, which may reveal missed drug hits in simple assay. We clinically approved platinum (Pt) and custom...
// Wen Zhang 1, * , Keli Ge Qian Zhao 2 Xiufen Zhuang 1 Zhenling Deng Lingling Liu Jie Li Yu Ying Dong Youhui Shuren Binlei 3 Department of Immunology, Cancer Institute & Hospital, Chinese Academy Medical Sciences Peking Union College, Beijing 100021, China Pathology, Hubei University Technology, Nanhu, Wuchang District, Wuhan 430068, These authors have contributed equally to this work Correspondence to: Liu, e-mail: liubinlei@cicams.ac.cn Zhang, zhangsr@cicams.ac.cn Keywords: oncolytic...
// Huiru Wang 1 , Qing Sun 2 Yanhong Wu Lin 3 Chunxia Zhou Wenbo Ma Youhui Zhang Shengdian 4 and Shuren Department of Immunology, Cancer Hospital & Institute, Peking Union Medical College Chinese Academy Sciences, Beijing, China Parasitology, Capital University, Pathology, Center Infection Immunity, Institute Biophysics, Correspondence to: Zhang, email: Keywords : cancer, granzyme M, chemoresistance, metastasis, epithelial-mesenchymal transition Received September 28, 2014 Accepted January...