A5087343743- Mesenchymal stem cell research
- Virus-based gene therapy research
- Cancer Research and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Glioma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Liver physiology and pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Hedgehog Signaling Pathway Studies
- CAR-T cell therapy research
- Organ Transplantation Techniques and Outcomes
- Cancer Cells and Metastasis
- Epilepsy research and treatment
- Ferroptosis and cancer prognosis
- Electrospun Nanofibers in Biomedical Applications
- Cancer Genomics and Diagnostics
- Neurological Disease Mechanisms and Treatments
- Nerve injury and regeneration
- Lipid Membrane Structure and Behavior
Ajou University
2008-2023
Seoul National University
2016
Transplantation of mesenchymal stem cells (MSCs) has been shown to enhance the recovery brain functions following ischemic injury. Although immune modulation suggested be one mechanisms, molecular mechanisms underlying improved not clearly identified. Here, we report that MSCs secrete transforming growth factor-beta (TGF-β) suppress propagation in rat brain. Ischemic stroke caused global death resident infarcted area, elevated monocyte chemoattractant protein-1 (MCP-1) level, and evoked...
Abstract Mesenchymal stem cells (MSCs) have been shown to ameliorate a variety of neurological dysfunctions. This effect is believed be mediated by their paracrine functions, since these rarely differentiate into neuronal cells. It clinical interest whether neural induction MSCs beneficial for the replacement therapy diseases. Here we report that expression Neurogenin1 (Ngn1), proneural gene directs differentiation progenitor during development, sufficient convert mesodermal cell fate one....
Bone marrow-derived mesenchymal stromal cells (MSCs) have been reported to be beneficial for the treatment of liver fibrosis. Here, we investigated use genetically engineered MSCs that overexpress hepatocyte growth factor (HGF) as a means improve their therapeutic effect in Liver fibrosis was induced by intraperitoneal injection dimethylnitrosamine. HGF-secreting (MSCs/HGF) were prepared transducing with an adenovirus carrying HGF-encoding cDNA. or MSCs/HGF injected directly into spleen...
Abstract Suicide genes have recently emerged as an attractive alternative therapy for the treatment of various types intractable cancers. The efficacy suicide gene relies on efficient delivery to target tissues and localized concentration final products. Here, we showed a potential ex vivo that used mesenchymal stem cells (MSCs) cellular vehicles deliver bacterial gene, cytosine deaminase (CD) brain tumors. MSCs were engineered produce CD enzymes at levels using different promoters. When...
We investigated a therapeutic strategy for recurrent malignant gliomas using mesenchymal stem cells (MSC), expressing cytosine deaminase (CD), and prodrug 5-Fluorocytosine (5-FC) as more specific less toxic option. MSCs are emerging novel cell agent with cancer-targeting property, CD is considered promising enzyme in cancer gene therapy which can convert non-toxic 5-FC to 5-Fluorouracil (5-FU). Therefore, use of minimize normal toxicity. Analyses microarrays revealed that targeting DNA...
Human mesenchymal stem cells (MSCs) are multipotent that have been intensively studied as therapeutic tools for a variety of disorders. To enhance the efficacy MSCs, genes introduced using retroviral and lentiviral vectors. However, serious adverse events (SAEs) such tumorigenesis can be induced by insertional mutagenesis. We generated vectors encoding wild-type herpes simplex virus thymidine kinase (HSV-TK) gene containing point mutation results in an alanine to histidine substitution at...
Human mesenchymal stem cells (MSCs) have emerged as attractive cellular vehicles to deliver therapeutic genes for ex-vivo therapy of diverse diseases; this is, in part, because they the capability migrate into tumor or lesion sites. Previously, we showed that MSCs could be utilized a bacterial cytosine deaminase (CD) suicide gene brain tumors. Here assessed whether transduction with retroviral vector encoding CD altered cell property MSCs. were transduced at passage 1 and cultivated up 11....
Human mesenchymal stem cells (MSCs) are currently being evaluated as a cell-based therapy for tissue injury and degenerative diseases.Recently, several methods have been suggested to further enhance the therapeutic functions of MSCs, including genetic modifications with tissue-and/or diseasespecific genes.The objective this study was examine efficiency stability transduction using an adenoviral vector in human MSCs.Additionally, we aimed assess effects on proliferation multipotency MSCs.The...
Therapeutic efficacy of mesenchymal stem cells (MSCs) is determined by biodistribution and engraftment in vivo. Compared to intravenous infusion, locally transplanted MSCs are partially understood. Here, we performed a pharmacokinetics (PK) study after local transplantation. We grafted human into the brains immune-compromised nude mice. Then extracted genomic DNA from brains, lungs, livers transplantation over month. Using quantitative polymerase chain reaction with Alu-specific primers,...
Abstract Mesenchymal stem cells (MSC) are being developed as therapeutic agents for treatment of various diseases due to their high tropism damaged sites and cancers, low immunogenicity. We developing technologies efficiently transfer genes including suicide that will be used suppress tumor growth or ensure safety against possible adverse events develop off-the-shelf formulations allogeneic uses. MSCs were engineered express a gene encoding non-human cytosine deaminase (CD) enzymes. aimed...
GNAO1 encodes the alpha subunit of heterotrimeric Go protein. Despite being most abundant G protein at synapses, role in brain remains unclear, primarily because high mortality associated with developmental and epileptic encephalopathy (DEE) 17 Gnao1 mutated animals. Here, we conducted proteomic analyses a synaptosomal fraction to investigate Go-interactome then generated non-DEE model using Gli1 CreERT2 mice selectively knockout (KO) presynaptic Gαo within cerebellum. Our findings revealed...
Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro. Here, we explored need using selection markers increase delivery efficiency and evaluated risks associated with their use manufacturing process. We used MSCs/CD that carry cytosine deaminase (CD) puromycin resistance (PuroR) marker. correlation between efficacy purity examining anti-cancer effect on co-cultured...
Stem cells carrying a suicide gene have emerged as therapeutic candidates for their cytotoxic bystander effects on neighboring cancers, while being non-
Several limitations are associated with the early diagnosis and treatment of incidental lower-grade glioma (iLGG), due to its unknown molecular features, management is categorized as either "wait-and-see" strategy or immediate treatment. Therefore, in this study authors explored iLGG's clinical landscape improve management.The retrospectively assessed differences between characteristics iLGG symptomatic (sLGG) samples filtered based on symptom data corresponding The Cancer Genome Atlas...
Jung-Mi Choi, Rakshya Acharya, Subash Marasini, Bashyal Narayan, Kwang-Wook Lee, Woo Sup Hwang, Da-Young Chang, Sung-Soo Kim and Haeyoung Suh-Kim. Exp Neurobiol 2021;30:203-12. https://doi.org/10.5607/en21017
The robust development of therapeutic strategies for many types cancer treatment has achieved remarkable outcomes in preclinical and clinical fields. One the approaches is based on suicide gene therapy using cytosine deaminase (CD) non-human origin a prodrug 5-flurocytosine (5-FC). CD enzymes convert non toxic 5-FC into anticancer drug, 5-flurouracil (5-FU), which induces cytotoxic bystander effect tumor cells. Previous studies demonstrated that mesenchymal stem cells show strong tropism...
Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor, current standard therapy provides modest improvement in progression-free overall survival of patients. The innate resistance invasiveness tumor presence blood-brain barrier (BBB) require development multi-modal therapeutic regimens. Previously, we demonstrated that cytosine deaminase (CD)-expressing mesenchymal stem cells (MSC/CD) exerted an anticancer activity with a minimized index by converting...
e14050 Background: Suicide genes induce apoptosis not only in the cell itself but also surrounding cells. The former is called suicide effect and latter bystander effect. Two major gene strategies currently pursued, cytosine deaminase (CD)/5-fluorocytosine (5-FC) herpes simplex virus-thymidine kinase/ganciclovir. Both have similar potential to effects, higher CD/5-FC. Here we show that level of CD expression/activity influence anti-cancer activity CD-expressing cells further demonstrate need...