Berberine, a naturally occurring isoquinoline alkaloid, has been shown to possess anti-inflammatory and antitumor properties in some vitro systems. Here, we report that treatment of androgen-insensitive (DU145 PC-3) androgen-sensitive (LNCaP) prostate cancer cells with berberine inhibited cell proliferation induced death dose-dependent (10-100 micromol/L) time-dependent (24-72 hours) manner. Treatment nonneoplastic human epithelial (PWR-1E) under identical conditions did not significantly...

Abstract: Dietary antioxidants protect laboratory animals against the induction of tumours by a variety chemical carcinogens. Among possible mechanism protection carcinogenesis could be mediated via‐antioxidant‐dependent detoxifying enzymes. Curcumin, yellow pigment from Curcuma longa , is major component turmeric and commonly used as spice food colouring material exhibits antiinflammatory, antitumour, antioxidant properties. In this study we therefore investigated effect dietary...

Abstract We have shown previously that dietary grape seed proanthocyanidins (GSP) inhibit UVB-induced photocarcinogenesis in mice. As oxidative stress and stress–mediated signaling has been implicated photocarcinogenesis, this study was designed to investigate the effect of GSPs on vivo SKH-1 hairless Here, we report provision (0.2 0.5%, w/w) mice exposed either acute UVB irradiation (120 mJ/cm2) or chronic inhibited depletion glutathione peroxidase, catalase, glutathione, H2O2, lipid...

Glutathione (GSH) plays several important roles in the protection of cells against oxidative damage, particularly following exposure to xenobiotics. Ferric nitrilotriacetate (Fe-NTA) is a potent depletor GSH and also enhances tissue lipid peroxidation. In this study, we show effect Fe-NTA treatment on hepatic some glutathione metabolizing enzymes, oxidant generation liver damage. The level activities reductase, S-transferase, peroxidase, glucose 6-phosphate dehydrogenase all decrease...

Chemotherapeutic approach using non-toxic botanicals may be one of the strategies for management skin cancers. Here we report that in vitro treatment human epidermoid carcinoma A431 cells with berberine, a naturally occurring isoquinoline alkaloid, decreased cell viability (3–77%, P < 0.05–0.001) and induced death (3–51%, 0.01–0.001) dose (5–75 μM)- time (12–72 h)-dependent manner, which was associated an increase G 1 arrest. 0 /G phase cycle is known to controlled by cyclin dependent...

The transition metal complexes of Co(II), Ni(II), Cu(II) and Zn(II) with bidentate Schiff base ligands (HL 1−5 ) were obtained from condensation 1, 2, 3, 4‐tetrahydro‐naphthalen‐1‐ylamine methanolic solution 3‐hydroxy‐salicylaldehyde, 4‐hydroxy‐salicylaldehyde, 5‐bromo‐salicylaldehyde, 3,5‐dibromo‐salicylaldehyde, 2‐hydroxy‐1‐napthaldehyde. synthesized compounds structurally elucidated by spectral physical methods. characterization emphasized nature (NO) which gets chelated via nitrogen atom...

Lung cancer remains the leading cause of cancer-related deaths worldwide, and non-small cell lung (NSCLC) represents approximately 80% total cases. The use non-toxic dietary phytochemicals can be considered as a chemotherapeutic strategy for management NSCLC. Here, we report that grape seed proanthocyanidins (GSPs) induce apoptosis NSCLC cells, A549 H1299, in vitro which is mediated through increased expression pro-apoptotic protein Bax, decreased anti-apoptotic proteins Bcl2 Bcl-xl,...

Overexpression of cyclooxygenase-2 (COX-2) and prostaglandins (PG) is linked to a wide variety human cancers. Here, we assessed whether the chemotherapeutic effect grape seed proanthocyanidins (GSP) on non-small cell lung cancer (NSCLC) cells mediated through inhibition COX-2 PGE(2)/PGE(2) receptor expression. The effects GSPs NSCLC lines in terms proliferation, apoptosis, expression COX-2, PGE(2), PGE(2) receptors were determined using Western blotting, fluorescence-activated sorting...

Abstract Phytochemicals have shown promise in inhibiting UV‐induced oxidative stress, and therefore are considered as potent inhibitors of stress‐mediated skin diseases. We previously that topical treatment silymarin, a flavonoid from milk thistle ( Silybum marianum ), inhibits stress mouse skin. However, the cellular targets responsible for inhibition by silymarin not clearly defined. To address this issue, C3H/HeN mice were UV irradiated (90 mJ cm −2 ) with or without (1 mg area). Mice...

Dietary grape seed proanthocyanidins (GSP) inhibit photocarcinogenesis in mice; however, the molecular mechanisms underlying this effect have not been fully elucidated. As ultraviolet B (UVB)-induced DNA damage form of cyclobutane pyrimidine dimers (CPDs) has implicated skin cancer risk, we studied whether dietary GSPs enhance repair UVB-induced and, if so, what is potential mechanism? Supplementation (0.5%, w/w) with AIN76A control diet significantly reduced levels CPD(+) cells UVB-exposed...

We have shown previously that dietary grape seed proanthocyanidins (GSPs) inhibit UVB-induced photocarcinogenesis in mice. As immune suppression has been implicated the development of skin cancer risk, we investigated whether GSPs can modulate effects UVB on system. found (180 mJ/cm2) ear swelling response (inflammatory reaction) was significantly lower mice fed with a GSP-supplemented (0.5 and 1.0%, w/w) diet than standard AIN76A diet. Dietary markedly inhibited contact hypersensitivity...

We designed the present study to investigate role of gentisic acid in chemopreventive activity Hibiscus rosa sinensis extract on 7,12-dimethyl benz(a)anthracene (DMBA)/croton oil-mediated carcinogenesis mouse skin via 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced tumour promotion response and oxidative stress. Single topical application DMBA followed by twice weekly applications croton oil after one week for 20 weeks resulted 100% incidence tumours animals 15 weeks. However, H. 30...

Lithium (Li+) salts are commonly used in treating bipolar diseases. As physicians frequently keep the patients on long-term lithium therapy, awareness of numerous side effects and pathogenesis this lightest alkali metal is needed for such treatments. The current study was designed to evaluate toxic effect small doses nitrate rats. In present we showed that oral gavage feeding (20 mg Li/kg body wt) 7 weeks every alternate day male albino wistar rats elicited a significant alterations gross...

Abstract Mixed ligand complexes of copper polyamine with biomolecules such as imidazole, substituted imidazoles or pyridine have been synthesized and characterized. These molecules were used because their low toxicity high activity. found to possess a distorted octahedral microenvironment potential SOD mimicking The IC50 values for these the order 2‐90 μM. Pyridine imidazole most effective they lowest 2.1 6 μM respectively which are higher than value complex. Based on uric acid estimations,...

Interleukin (IL)-12 deficiency exacerbates tumorigenesis in ultraviolet (UV) radiation-induced skin. Here, we assessed the effects of IL-12 on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion 7,12-dimethylbenz(a)anthracene (DMBA)-initiated mouse Using this two-stage chemical carcinogenesis protocol, found that development DMBA/TPA-induced skin tumors was diminished IL-12p40-knockout mice than their wild-type counterparts. At termination experiment (at 24 weeks), incidence...

Previously, we have reported that aurintricarboxylic acid (ATA) is one of the most potent inhibitors DNA binding transcription factor NF-κB. We now report NF-κB-DNA inhibitory activity ATA analogues. An electrophoretic mobility shift assay has shown bromopyrogallol red (BPR) effective inhibitor among studied The molecular modeling studies showed BPR makes a strong network hydrogen bonds with DNA-binding region p50 subunit NF-κB and electronegative potential on its peripheral surface. Because...

Probucol, a clinically used cholesterol lowering and antioxidant drug, was investigated for possible protection against lipid peroxidation DNA damage induced by iron nitrilotriacetate (Fe-NTA) plus hydrogen peroxide (H2O2). Fe-NTA is potent nephrotoxic agent induces acute subacute renal proximal tubular necrosis catalyzing the decomposition of H2O2-derived production hydroxyl radicals, which are known to cause damage. associated with high incidence adenocarcinoma in rodents. Lipid principal...