A5042718579- Ultrasound and Hyperthermia Applications
- Ultrasound and Cavitation Phenomena
- Photoacoustic and Ultrasonic Imaging
- Ultrasound Imaging and Elastography
- Nanoparticle-Based Drug Delivery
- Electrohydrodynamics and Fluid Dynamics
- Nanoplatforms for cancer theranostics
- Microbial Inactivation Methods
- Bladder and Urothelial Cancer Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Spectroscopy and Chemometric Analyses
- Microfluidic and Bio-sensing Technologies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Microfluidic and Capillary Electrophoresis Applications
- Genital Health and Disease
- Insect symbiosis and bacterial influences
- Polyamine Metabolism and Applications
- Neonatal Respiratory Health Research
- Selenium in Biological Systems
- Advancements in Solid Oxide Fuel Cells
- Nanomaterials for catalytic reactions
- Histone Deacetylase Inhibitors Research
- Chemotherapy-induced organ toxicity mitigation
- Advanced Fluorescence Microscopy Techniques
- MRI in cancer diagnosis
University of Oxford
2014-2024
CRUK/MRC Oxford Institute for Radiation Oncology
2019-2021
University College London
2017-2021
University of Colorado Health
2021
University of Southampton
2021
Presbyterian St. Luke's Medical Center
2021
Science Oxford
2020
Cancer Research UK
2019
University of Colorado Denver
2019
Institute of Biomedical Science
2016-2017
The structure of microvasculature cannot be resolved using standard clinical ultrasound (US) imaging frequencies due to the fundamental diffraction limit US waves. In this work, we use a system perform in vivo sub-diffraction on CD1, female mouse aged eight weeks by localizing isolated signals from microbubbles flowing within ear microvasculature, and compare our results optical microscopy. Furthermore, develop new technique map blood velocity at super-resolution tracking individual bubbles...
Targeted delivery of potent, toxic chemotherapy drugs, such as cisplatin, is a significant area research in cancer treatment. In this study, cisplatin was successfully encapsulated with high efficiency (>70%) poly (lactic-co-glycolic acid) polymeric nanoparticles by using electrohydrodynamic atomization (EHDA) where applied voltage and solution flow rate well the concentration polymer were varied to control size particles. Thus, produced three different drug:polymer ratios (2.5, 5 10wt%...
The development of new modalities for high-efficiency intracellular drug delivery is a priority number disease areas. One such area urinary tract infection (UTI), which one the most common infectious diseases globally and imposes an immense economic healthcare burden. Common uropathogenic bacteria have been shown to invade urothelial wall during acute UTI, forming latent reservoirs that can evade antimicrobials immune response. This behaviour likely facilitates high recurrence rates after...
In order to maintain a stable cone-jet mode in electrospray low flow rates are used while most applications require high throughput. We compare two different designs of the multiple system increase output for large scale production applications. this study, solution was fed through four separate needles that were attached either circular or rectangular metallic plate connected voltage DC power supply. The behaviour jets as well deposition particles investigated. It shown throughput increased...
The high efficiency with which gas microbubbles can scatter ultrasound compared the surrounding blood pool or tissues has led to their widespread employment as contrast agents in imaging. In recent years, applications have been extended include super-resolution imaging and stimulation of localized bio-effects for therapy. growing exploitation particular these developments amplified need characterize fully understand microbubble behavior. aim doing so is more exploit utility both diagnostic...
Microbubbles are currently in clinical use as ultrasound contrast agents and under active investigation mediators of therapy. To improve the theranostic potential microbubbles, nanoparticles can be attached to bubble shell for imaging, targeting and/or enhancement acoustic response. Existing methods fabricating particle-loaded bubbles, however, require polymers, oil layers or chemical reactions particle incorporation; embed/attach particles that reduce echogenicity; impair biocompatibility;...
Abstract: Increasing the clinical efficacy of toxic chemotherapy drugs such as cisplatin (CDDP), via targeted drug delivery, is a key area research in cancer treatment. In this study, CDDP-loaded poly(lactic-co-glycolic acid) (PLGA) polymeric nanoparticles (NPs) were successfully prepared using electrohydrodynamic atomization (EHDA). The configuration was varied to control distribution CDDP within particles, and high encapsulation efficiency (>70%) achieved. NPs produced with either...
We describe a cost-effective and simple method to fabricate PDMS-based microfluidic devices by combining micromilling with replica moulding technology. It relies on the following steps: (i) microchannels are milled in block of acrylic; (ii) low-cost epoxy adhesive resin is poured over acrylic allowed cure; (iii) solidified layer peeled off used as mould for transferring microchannel architecture onto PDMS layer; finally (iv) plasma bonded glass surface. With this method, microscale...
Surfactant-coated gas microbubbles are widely used as contrast agents in ultrasound imaging and increasingly therapeutic applications. The response of to can be strongly influenced by their size coating properties, hence the production method. Ultrasonic emulsification (sonication) is most commonly employed method generate high concentrations rapidly, but with a broad distribution, there risk contamination and/or degradation sensitive components. Microfluidic devices provide excellent...
Abstract The G ulf‐ C oast tick, A mblyomma maculatum , possesses an elaborate set of selenoproteins, which prevent the deleterious effects from oxidative stress that would otherwise occur during feeding. In current work, we examined role selenoprotein K ( SelK ) and M SelM in feeding . by bioinformatics, transcriptional gene expression, RNA interference antioxidant assays. expression did not vary significantly salivary glands or midguts throughout bloodmeal. However, there was a 58‐fold...
Spectral imaging with polarity-sensitive fluorescent probes enables the quantification of cell and model membrane physical properties, including local hydration, fluidity, lateral lipid packing, usually characterized by generalized polarization (GP) parameter. With development commercial microscopes equipped spectral detectors, has become a convenient powerful technique for measuring GP other properties. The existing tools image processing, however, are insufficient processing large data...
Phospholipid coated microbubbles are currently in widespread clinical use as ultrasound contrast agents and under investigation for therapeutic applications. Previous studies have demonstrated the importance of coating nanostructure determining microbubble stability its dependence upon both composition processing method. While influence different phospholipids has been widely investigated, role other constituents such emulsifiers received comparatively little attention. Herein, we present an...
There are several limitations with monodrug cancer therapy, including poor bioavailability, rapid clearance and drug resistance. Combination therapy addresses these by exploiting synergism between different drugs against cells. In particular, the combination of epigenetic therapies conventional chemotherapeutic agents can improve initial tumour response overcome acquired Co-encapsulation multiple therapeutic into a single polymeric nanoparticle is one many approaches taken to enhance effect...
Abstract The Pt IV prodrug iproplatin has been actively loaded into liposomes using a calcium acetate gradient, achieving 3‐fold enhancement in drug concentration compared to passive loading strategies. A strain‐promoted cycloaddition reaction (azide‐ dibenzocyclooctyne) was used attach iproplatin‐loaded L(Pt) gas‐filled microbubbles (M), forming an ultrasound‐responsive delivery vehicle [M−L(Pt)]. Ultrasound‐triggered release of from the microbubble‐liposome construct evaluated cellulo....
PurposeChemoradiation therapy is the standard of care in muscle-invasive bladder cancer (MIBC). Although agents such as gemcitabine can enhance tumor radiosensitivity, their side effects limit patient eligibility and treatment efficacy. This study investigates ultrasound microbubbles for targeting delivery to reduce normal-tissue toxicity a murine orthotopic MIBC model.Materials MethodsCD1-nude mice were injected orthotopically with RT112 cells. Conventional chemoradiation involved injecting...
Abstract The signalling pathways in tick salivary glands that control ‘sialo‐secretome’ secretion at the tick−host interface remain elusive; however, this complex process is essential for successful feeding and manipulation of host haemostatic response. Exocytosis sialo‐secretome requires a core soluble N ‐ethylmaleimide‐sensitive fusion (NSF) attachment proteins ( SNAPs ) receptor SNAREs ). have been identified as key components regulating gland cells. In study, we utilized RNA interference...
Despite an overall improvement in survival rates for cancer, certain resistant forms of the disease still impose a significant burden on patients and healthcare systems. Standard chemotherapy these cases is often ineffective and/or gives rise to severe side effects. Targeted delivery chemotherapeutics could improve both tumour response patient experience. Hence, there urgent need develop effective methods this. Ultrasound established technique diagnosis therapy. Its use conjunction with...
Blepharochalasis is a rare disorder of unknown etiology defined by loose, atrophic periorbital skin following recurrent episodes eyelid edema. Characteristic histopathology shows complete absence elastic fibers. The current case progressed after multiple crying, which may be related to matrix metalloproteinase dysregulation. This offers further insights into the possible pathogenesis blepharochalasis, paving way for more targeted, disease-modifying therapies.
In previous work we demonstrated that microbubble mediated gene delivery can be enhanced in vitro through simultaneous exposure of cells to ultrasound and magnetic fields the presence magnetically loaded contrast agents. The aim this preliminary study was investigate feasibility technique for vivo applications. Phospholipid coated microbubbles with a hydrocarbon suspension nanoparticles were prepared sonication sized using optical microscopy (concentration 1.4 x 108 bubbles/ml). Plasmid...