A5052448080- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune cells in cancer
- Immune Cell Function and Interaction
- Lung Cancer Treatments and Mutations
- Glioma Diagnosis and Treatment
- Chemokine receptors and signaling
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Hypoxia, and Metabolism
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- T-cell and B-cell Immunology
- Advanced Nanomaterials in Catalysis
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Neurological disorders and treatments
- Advanced biosensing and bioanalysis techniques
- HER2/EGFR in Cancer Research
- Phagocytosis and Immune Regulation
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Transcranial Magnetic Stimulation Studies
- RNA Interference and Gene Delivery
Ningbo University
2024-2025
Harbin Medical University
2014-2024
Fourth Affiliated Hospital of Harbin Medical University
2016-2024
Stanford University
2020-2023
Ningbo University Affiliated Hospital
2023
Fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) holds great promise for deep tissue visualization. Development of novel clinical translatable NIR-II probes is crucial realizing medical applications fluorescence imaging. Herein, glutathione-capped gold nanoclusters (AuNCs, specifically Au25 (SG)18 ) demonstrate highly efficient binding capability to hydroxyapatite vitro first time. Further vivo AuNCs indicate that they accumulate bone tissues with high contrast...
Tumor heterogeneity and changes in epidermal growth factor receptor (EGFR) mutation status over time challenge the design of effective EGFR tyrosine kinase inhibitor (TKI) treatment strategies for non-small cell lung cancer (NSCLC). Therefore, there is an urgent need to develop techniques comprehensive tumor profiling real time, particularly precision medicine trials. We report a positron emission tomography (PET) tracer, N-(3-chloro-4-fluorophenyl)-7-(2-(2-(2-(2-18F-fluoroethoxy) ethoxy)...
Abstract Purpose: Immunomonitoring of chimeric antigen receptor (CAR) T cells relies primarily on their quantification in the peripheral blood, which inadequately quantifies biodistribution and activation status tissues. Noninvasive molecular imaging CAR by PET is a promising approach with ability to provide spatial, temporal, functional information. Reported strategies rely incorporation reporter transgenes or ex vivo biolabeling, significantly limiting application T-cell imaging. In this...
Abstract When properly deployed, the immune system can eliminate deadly pathogens, eradicate metastatic cancers, and provide long‐lasting protection from diverse diseases. Unfortunately, realizing these remarkable capabilities is inherently risky as disruption to homeostasis elicit dangerous complications or autoimmune disorders. While current research continuously expanding arsenal of potent immunotherapeutics, there a technological gap when it comes controlling when, where, how long drugs...
Activated T cells play a pivotal role in rheumatoid arthritis (RA) pathogenesis, and imaging of activated may provide non-invasive tool for RA detection. Here, we first developed an optical probe targeting human inducible cell co-stimulator (ICOS) tested its capacity diagnosis by capturing ICOS+ vivo humanized mouse model. The model, Human peripheral blood mononuclear cells- adjuvant induced (HuPBMC-AIA) was established, flow cytometry immunofluorescence were employed to determine ICOS...
Abstract Purpose: Immunotherapy is a promising approach for many oncological malignancies, including glioblastoma, however, there are currently no available tools or biomarkers to accurately assess whole-body immune responses in patients with glioblastoma treated immunotherapy. Here, the utility of OX40, costimulatory molecule mainly expressed on activated effector T cells known play an important role eliminating cancer cells, was evaluated as PET imaging biomarker quantify and track...
To prepare and evaluate a new radiotracer 18F-IRS for molecular imaging mutant EGF Receptors in vitro vivo. Uptake efflux of were performed with four NSCLC cell lines including HCC827, H1975, H358 H520. In vivo tumor targeting pharmacokinetics the radiotracers also evaluated H520 tumor-bearing nude mice by PET/CT imaging. Ex biodistribution assays to quantify accumulation We three patients NSCLC. labeled this small molecule QD620 flow cytometry confocal analyses. The uptakes HCC827 tumors...
Elevated expression of the c-Met receptor plays a crucial role in cancers. In non–small cell lung cancer (NSCLC), aberrant activation signaling pathway contributes to tumorigenesis and progression may mediate acquired resistance epidermal growth factor receptor–targeted therapy. is therefore emerging as promising therapeutic target for NSCLC, methods noninvasive vivo assessment would improve NSCLC treatment diagnosis. <b>Methods:</b> We developed new c-Met–binding peptide (cMBP) radiotracer,...
Abstract Background Liver kinase B1 ( LKB1 ) is frequently mutated in lung adenocarcinoma, and its loss contributes to tumor progression. Methods To identify downstream genes that promote adenocarcinoma aggressiveness, we performed bioinformatical analysis using publicly available datasets. Results Rab3B was upregulated -depleted cells suppressed by overexpression. CREB protein enriched at the promoter of cancer cells. Silencing abrogated upregulation upon loss. Immunohistochemistry revealed...
We first developed a 4-1BB-targeted optical probe, named IRDye-680RD-4-1BB mAb (monoclonal antibody), and evaluated its value for the detection of 4-1BB+ activated T cells in vivo as well diagnosis rheumatoid arthritis (RA) an adjuvant-induced (AIA) mouse model.The 4-1BB expression pattern was analysed by flow cytometry immunofluorescence (IF) staining. The conjugated with IRDye-680RD NHS ester, characterized via fluorescence spectrum. A cell-binding assay also performed to assess...
Tyrosine kinase inhibitors (EGFR-TKIs) targeting the epidermal growth factor receptor (EGFR) have been used in non-small cell lung carcinoma (NSCLC) for years with promising results, particular patients activating mutations EGFR domain (exon 19 E746-A750 deletion or exon 21 L858R point mutation). However, despite their great success clinic, a significant number of do not respond to EGFR-TKIs, such as those carrying L858R/T790M mutation wild type. Thus, detecting status before EGFR-TKIs...
Background: Electrode reconstruction for postoperative deep brain simulation (DBS) can be achieved manually using a surgical planning system such as Surgiplan, or in semi-automated manner software the Lead-DBS toolbox. However, accuracy of has not been thoroughly addressed. Methods: In our study, we compared DBS results and Surgiplan. We included 26 patients (21 with Parkinson’s disease 5 dystonia) who underwent subthalamic nucleus (STN)-DBS, reconstructed electrodes toolbox The electrode...
The goal of this study was to develop an imaging probe-IRDye-680RD-OX40 mAb-that can be used for noninvasive and optical rheumatoid arthritis (RA). OX40/OX40 ligand (OX40L) interactions have been shown exert potent costimulatory effects on T cell activation. Detectable change in activation profiles observed early RA.OX40 expression pattern analyzed by flow cytometry. N-hydroxysuccinimide (NHS) esters are label proteins selectively free amino groups OX40 monoclonal antibody (mAb)....
Diagnosis of organ transplant rejection relies upon biopsy approaches to confirm alloreactive T cell infiltration in the graft. Immune molecular monitoring is under investigation screen for rejection, though these techniques have suffered from low specificity and lack spatial information. ImmunoPET utilizing antibodies conjugated radioisotopes has potential improve early accurate detection graft rejection. capable noninvasively visualizing dynamic distribution cells expressing specific...
Background: Colorectal cancer is a highly aggressive malignant tumor that primarily affects the digestive system. It frequently diagnosed at an advanced stage. Cuproptosis copper-dependent form cell death mechanism, distinct from all other known pathways underlying death, progression, prognosis, and immune response. Although role of cuproptosis in colorectal has been investigated over time, there still urgent need to explore new methods insights understand its potential function. Methods:...
<title>Abstract</title> <bold>Background</bold> Activated T cells play a pivotal role in rheumatoid arthritis (RA) pathogenesis, and imaging of activated may provide non-invasive tool for RA detection. Here, we first developed an optical probe targeting human ICOS tested its capacity diagnosis by capturing ICOS<sup>+</sup> <italic>in vivo</italic> humanized mouse model. <bold>Methods</bold> The model, HuPBMC-AIA (Human peripheral blood mononuclear cells- adjuvant induced arthritis) was...