A5101590407- Congenital heart defects research
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Tea Polyphenols and Effects
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer-related gene regulation
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- Advanced Glycation End Products research
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Retinal Diseases and Treatments
- Piperaceae Chemical and Biological Studies
- Nerve injury and regeneration
- Tryptophan and brain disorders
- RNA Interference and Gene Delivery
- Coenzyme Q10 studies and effects
- Congenital Heart Disease Studies
- Cardiovascular Disease and Adiposity
- Wound Healing and Treatments
Chongqing Medical University
2010-2023
Children's Hospital of Chongqing Medical University
2010-2023
China International Science and Technology Cooperation
2020-2023
National Clinical Research
2020
Zhengzhou University
2020
China-US (Henan) Hormel Cancer Institute
2020
Fuling Center Hospital of Chongqing
2015
Eye & ENT Hospital of Fudan University
2011
Fudan University
2008
The metabolic patterns and energetics of human induced pluripotent stem cell-derived cardiomyocytes (HiPSC-CMs) are much less than those normal adult cardiomyocytes, which has limited their application in disease therapy regenerative medicine. It been demonstrated that SIRT3, a mitochondria-target deacetylase, controls mitochondrial metabolism physiological pathological conditions. In this research, We investigated the role regulatory mechanism SIRT3 energy HiPSC-CMs. found expression was...
The occurrence of Congenital Heart Disease (CHD) is resulted from either genetic or environmental factors the both. CITED2 gene deletion mutation associated with development cardiac malformations. In this study, we have investigated role and methylation in pediatric patients China. We screened 120 congenital heart disease. Among these patients, 4 cases were detected to carry various heterozygous mutations (c.550G > A, c.574A G, c.573-578del6) leading correspondingly alterations amino acid...
Identifying intravenous immunoglobulin-resistant patients is essential for the prompt and optimal treatment of Kawasaki disease, suggesting need effective risk assessment tools. Data-driven approaches have potential to identify high-risk individuals by capturing complex patterns real-world data. To enable clinically applicable prediction immunoglobulin resistance addressing incompleteness clinical data lack interpretability machine learning models, a multi-stage method developed integrating...
Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs) (hiPSC-CMs) are a promising source for disease modeling, myocardial regeneration, and drug assessment. However, hiPSC-CMs have certain immature fetal CM-like properties that different from the characteristics of adult CMs in several aspects, including cellular structure, mitochondrial function, metabolism, thus limiting their applications. Adenosine 5‘-monophosphate (AMP)-activated protein kinase (AMPK) is an...
Despite considerable efforts, mTOR inhibitors have produced limited success in the clinic. To define vulnerabilities of mTORC1-addicted cancer cells and to find previously unknown therapeutic targets, we investigated mechanism piperlongumine, a small molecule identified chemical library screen specifically target with hyperactive mTORC1 phenotype. Sensitivity piperlongumine was dependent on its ability suppress RUVBL1/2-TTT, complex involved chromatin remodeling DNA repair. Cancer high...
Combining neurotrophic factor support and neural stem cell (NSC) transplantation may improve regeneration of the peripheral nervous system. Objectives. We constructed a biodegradable nerve conduit (NC) filled with NSCs overexpressing glia-derived (GDNF), which is known to protect facial motoneurons, tested effect this NC on regeneration.Primary cultured were transduced lentiviral vector encoding enhanced green fluorescent protein (EGFP) GDNF. GDNF expression was confirmed by Western blotting...
Heart failure is a common, costly, and potentially fatal condition. The cardiac sarcoplasmic reticulum Ca-ATPase (SERCA2a) plays critical role in the regulation of function. Previously, low SERCA2a expression was revealed mice with heart failure. Epigallocatechin-3-gallate (EGCG) can function as an epigenetic regulator has been reported to enhance However, underlying regulatory mechanism still unclear. In this study, we investigated whether EGCG up-regulate via histone acetylation play...
Abstract Background Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for regenerative medicine and in drugs screening. Despite displaying key cardiomyocyte phenotypic characteristics, they more closely resemble fetal/neonatal are still immature; these cells mainly rely on glucose as a substrate metabolic energy, while mature employ oxidative phosphorylation of fatty acids. Studies showed that the alteration metabolism pattern from glycolysis to...
This study was to test the hypothesis that oxidized low-density lipoprotein (ox-LDL) modified behavior of bone marrow stem cells, including proliferation, Oct-4 expression, and their endothelial differentiation through reactive oxygen species (ROS) formation in vitro. Rat multipotent adult progenitor cells (MAPCs) were treated with ox-LDL or without antioxidant N-acetylcysteine (NAC). Ox-LDL generated a significant amount ROS culture system as measured electron paramagnetic resonance...
Abstract Background Microglial activation and the proinflammatory response are controlled by a complex regulatory network. Among various candidates, macrophage colony-stimulating factor (M-CSF) is considered an important cytokine. The up-regulation of M-CSF its receptor CSF-1R has been reported in brain disease, as well diabetic complications; however, mechanism unclear. An elevated level glycated albumin (GA) characteristic diabetes; thus, it may be involved monocyte/macrophage-associated...
Abstract Mesenchymal stem cells (MSCs) specifically differentiate into cardiomyocytes as a potential way to reverse myocardial injury diseases, and uncovering this differentiation mechanism is immensely important. We have previously shown that histone acetylation/methylation DNA methylation are involved in MSC induced by islet-1. These modifications regulate cardiac-specific genes interacting with each other the promoter regions of these genes, but molecular interactions remains unknown. In...
The aim of the present study was to investigate effects Islet-1 on process mesenchymal stem cell (MSC) differentiation into cardiomyocyte-like cells and elucidate possible mechanisms involved. Lentiviral vectors expressing (Lenti-Islet-1) were constructed used for C3H10T1/2 transfection. Cell morphology observed. Cardiac-related genes proteins detected by qPCR western blot analysis. Epigallocatechin gallate (EGCG) as an inhibitor acetylated histone H3 (AcH3). AcH3 chromatin...
This study was to determine if bone marrow multipotent adult progenitor cells (MAPCs) underwent cardiac specification and Oct-4 expression during their cardiomyocyte differentiation in vitro. MAPCs were isolated from rat marrow, treated with 5-azacytidine (5-aza, 1μM) for 24h, cultured a serum-free medium up 35 days. The started express early cardiac-specific genes Nkx2.5 GATA-4 significant increase mRNA level within 24h after 5-aza treatment. Western blotting analysis immunofluorescence...
Bone morphogenetic protein (BMP)‑2 induces the expression of cardiac transcription factors during early heart development, however, underlying mechanisms for this are not clear. Our previous studies indicated that histone acetylation is critical in regulation gene expression. In present study, hypothesis BMP2 enhances by increasing H3 was tested. Cultured H9c2 rat embryonic myocytes were transfected with adenoviruses expressing human (AdBMP2). Real‑time RT‑PCR, western blotting, chromatin...
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are seed cells that can be used for alternative treatment of myocardial damage. However, their immaturity limits clinical application. Mitochondrial development accompanies cardiomyocyte maturation, and PINK1 plays an important role in the regulation mitochondrial quality. mechanism remain unclear.We proteomic phosphoproteomic to identify protein phosphosite changes hiPSC-CMs deficient PINK1. Bioinformatics analysis was...
The aim of this study was to investigate whether Treg/Th17 ratio regulation plays an important role in epigallocatechin-3-gallate (EGCG) attenuating increased afterload-induced cardiac hypertrophy. Three-month-old male C57BL/6 mice were divided into sham + vehicle, abdominal aortic constriction (AAC) and AAC EGCG groups. Intraperitoneal (50 mg/kg/d) administration conducted. Cardiac structure function examined by ultrasonography. Pathology hematoxylin eosin staining, wheat germ agglutinin...
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are cells with promising applications. However, their immaturity has restricted use in cell therapy, disease modeling, and other studies. Therefore, the current study focused on inducing maturation of CMs. We supplemented hiPSC-CMs fatty acids (FAs) to promote phenotypic maturity. Proteomic sequencing was performed identify regulators critical for promoting hiPSC-CMs. AKAP1 found be significantly increased FA-treated...
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have attracted attention in the field of regenerative medicine due to their potential ability repair damaged hearts. However, immature phenotype these cells limits clinical application. Cardiomyocyte maturation is accompanied by changes mitochondrial quality. PTEN-induced putative kinase 1 (PINK1) has been linked quality control. whether hiPSC-CMs are associated with PINK1, and impact PINK1 on development not clear. In...
Mesenchymal stem cells (MSCs) can treat myocardial injury-related diseases by differentiating into cardiomyocytes. Islet-1 plays an essential role in cardiac maturation. We have discovered that a crucial the histone acetylation regulation this process. In addition, to increase GATA4/Nkx2.5 expression, may bind Gcn5 and then guide promoters, thereby facilitating differentiation of MSCs is important factor maturation heart. previously found pivotal process Islet-1. Furthermore, boost which...
Objective To compare the changes in myocardial fibrosis degree and left ventricular function before versus after percutaneous coronary intervention (PCI) elderly patients with heart disease (CHD). Methods 121 diagnosed as CHD a single vessel by angiography were enrolled. All treated PCI guided thrombolysis ischemia (TIMI) grade, symptoms fractional flow reserve (FFR) comprehensively, reviewed 12 months. The serum concentration of procollagen type I (PC I), Ⅲ Ⅲ), laminin (LN),...
Abstract A deficiency of striated preferentially expressed gene ( Speg ), a member the myosin light chain kinase family, results in abnormal myofibril structure and function immature cardiomyocytes (CMs), corresponding with dilated cardiomyopathy, heart failure perinatal death. Mitochondrial development plays role cardiomyocyte maturation. Therefore, this study investigated whether – / ) CMs would result mitochondrial abnormalities. wild-type −/− C57BL/6 littermate mice were utilized for...
Heart failure is becoming an increasingly common and significant problem in the field of pediatric cardiology,β-blockers as one most important cardiovascular drags pharmacopoeia,can not only obviously relieve symptoms severe heart failure,but also reduce events allcause mortality significantly,the efficacy effective high security.This article reviewed β-blockers treatment children mechanism,the clinical application evaluation,the basic principles relevant issues use process,the appropriate...
Abstract Background Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) hold great promise for regenerative medicine and in vitro screening. Despite displaying key cardiomyocyte phenotypic characteristics, they more closely resemble fetal/neonatal are still immature, these cells mainly rely on glucose as a substrate metabolic energy, while mature employ oxidative phosphorylation of fatty acids. Studies showed that the alteration metabolism pattern from glycolysis to improve...