A5084933395- Computational Drug Discovery Methods
- Alzheimer's disease research and treatments
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Functional Brain Connectivity Studies
- Cell Image Analysis Techniques
- Bioinformatics and Genomic Networks
- Microtubule and mitosis dynamics
- Chemical synthesis and alkaloids
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Treatment of Major Depression
- Schizophrenia research and treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Force Microscopy Techniques and Applications
- Mitochondrial Function and Pathology
- Gene Regulatory Network Analysis
- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Memory and Neural Mechanisms
- Neurological disorders and treatments
Certara (United States)
2020-2025
Predictive Science (United States)
2024-2025
Integra LifeSciences (United States)
2023-2024
In Silico Biosciences (United States)
2012-2021
University of Pennsylvania
2005-2018
Sherwin Williams (United States)
2016
Dialyse Centrum Groningen
2012
Cold Spring Harbor Laboratory
2005
Institute on Aging
2005
Janssen (Belgium)
1992-2002
This paper presents the measurement of colored object reflectance, under different, general assumptions regarding imaging conditions. We exploit Gaussian scale-space paradigm for color images to define a framework robust reflectance from images. Object is derived physical model based on Kubelka-Munk theory colorant layers. Illumination and geometrical invariant properties are model. Invariance discriminative power invariants experimentally investigated, showing be successful in discounting...
Protein tau filaments in brain of patients suffering from Alzheimer's disease, frontotemporal dementia, and other tauopathies consist protein that is hyperphosphorylated. The responsible kinases operating vivo neurons still need to be identified. Here we demonstrate glycogen synthase kinase-3β (GSK-3β) an effective kinase for cerebral adult GSK-3β × human tau40 transgenic mice. Phosphorylated migrates slower during electrophoretic separation revealed by phosphorylation-dependent anti-tau...
A critical step in automatic microscopy is focusing. This report describes a robust and fast autofocus approach useful for wide range of microscopic modalities preparations.The focus curve measured over the complete focal range, reducing chance that best position determined by dust or optical artifacts. Convolution with derivative Gaussian smoothing function reduces effect noise on curve. The influence mechanical tolerance accounted for.The method shown to be fluorescence, bright-field phase...
Abstract Transferrin receptors labeled with the B3/25 monoclonal antibody–gold complexes were followed in living A431 cells by using video‐enhanced contrast microscopy. Initially, antibody‐gold bind to which are freely mobile on upper cell surface; they then become trapped at inner margins of peripheral lamellae and internalize. During endocytosis discrete gold‐loaded vesicular elements first appear, then, as fuse, a heterogenous endosomal compartment forms. The endosomes from this begin...
A recently introduced extension of video-enhanced light microscopy, called Nanovid documents the dynamic reorganization individual cell surface components on living cells. 40-microns colloidal gold probes coupled to different types poly-L-lysine label negative PTK2 Evidence is provided that they bind sialic acid residues glycoproteins, probably through nonspecific electrostatic interactions. The probes, short molecules (4 kD) displayed Brownian motion, with a diffusion coefficient in range...
Abstract Introduction The longitudinal trajectories of functional brain dynamics and the impact genetic risk factors in individuals at for Alzheimer's disease are poorly understood. Methods In a large‐scale monocentric cohort 224 amyloid stratified disease, default mode network (DMN) resting state connectivity (FC) was investigated between two serial time points across 2 years. Results Widespread DMN FC changes were shown frontal posterior areas, as well right hippocampus. There no...
Mutant human presenilin-1 (PS1) causes an Alzheimer's-related phenotype in the brain of transgenic mice combination with mutant amyloid precursor protein by means increased production peptides (Dewachter, I., Van Dorpe, J., Smeijers, L., Gilis, M., Kuiperi, C., Laenen, Caluwaerts, N., Moechars, D., Checler, F., Vanderstichele, H. & Leuven, F. (2000) <i>J. Neurosci.</i> 20, 6452–6458) that aggravate plaques and cerebrovascular (Van Dewachter, Nuyens, Spittaels, K., van den Haute, Mercken,...
Objectives: A QSP model was developed to better understand the systemic response avalglucosidase alfa in patients with Pompe disease, a rare disease caused by deficient lysosomal glycogen degradation. This describes both late-onset (LOPD) and severe infantile-onset (IOPD) phenotypes within single mechanistic framework. To supplement data from Mini-COMET (NCT03019406) study comparing vs. standard-of-care treatment IOPD patients, QSP-based virtual twin analysis performed account for low...
Apolipoprotein E <i>(APOE)</i> has been extensively demonstrated to be a genetic risk factor for Alzheimer’s disease (AD). Associations of <i>APOE</i> genotype have reported with age at AD onset, rate decline, and responsiveness therapy. This study aimed test these hypotheses in large population patients. was determined from 1,528 Caucasian subjects, diagnosed by NINCDS/ADRDA criteria as probable patients, enrolled four international placebo-controlled clinical trials...
There is increasing evidence that apoptosis in postmitotic neurons associated with a frustrated attempt to reenter the mitotic cycle. Okadaic acid, specific protein phosphatase inhibitor, currently used models of Alzheimer's research increase degree phosphorylation various proteins, such as microtubule-associated tau. acid induces programmed cell death human neuroblastoma lines TR14 and NT2-N, evidenced by fragmentation DNA attenuation this process synthesis inhibitors. In differentiated...
In Alzheimer's disease and related dementias, human tau protein aggregates into paired helical filaments neurofibrillary tangles. However, such have not yet been demonstrated in transgenic mouse models of the disease. One possible explanations would be that has different properties which prevents it from aggregating. We cloned several murine isoforms, containing three or four repeats combinations inserts, expressed them Escherichia coli show here they can all assembled similar to those...