The Ca2+ accumulating properties of a nonmitochondrial intracellular organelle within cultured N1E-115 neuroblastoma cells containing an (ATP + Mg2+)-dependent pump were recently described in detail (Gill, D. L., and Chueh, S. H. (1985) J. Biol. Chem. 260, 9289-9297). Using both saponin-permeabilized microsomal membranes from cells, this report describes the effectiveness inositol 1,4,5-trisphosphate (IP3) guanine nucleotides mediating release internal organelle, believed to be endoplasmic...

A sensitive and specific guanine nucleotide regulatory process has recently been shown to rapidly mediate a substantial release of Ca2+ from endoplasmic reticulum within the N1E-115 neuronal cell line (Gill,

To determine more about the GTP-activated Ca2+ translocation process, effects of GTP on cells loaded with Ca-oxalate were investigated.Using permeabilized both N1E-115 neuroblastoma and DDT1MF-2 smooth muscle cell lines, 10 p M activates a profound uptake in presence oxalate, as opposed to release observed without oxalate.GTP stimulation was at oxalate concentrations (2 mM) only slightly augmenting GTP; 8 mM (which alone induces linear accumulation) still increases rate uptake.GTP-activated...

Two major Ca2+ transport mechanisms co-function in a preparation of synaptosomal plasma membrane vesicles: an (ATP + Mg2+)-dependent pump, and reversible Na+-Ca2+ exchanger (Gill, D. L., Grollman, E.F., Kohn, L. (1981) J. Biol. Chem. 256, 184-192). An accurate comparative analysis the kinetics two transporters under free conditions precisely buffered with EGTA, reveals that both have high affinity for Ca2+. The ATP-dependent pump displays simple saturation Km 0.11 microM Vmax 2.2 nmol/min/mg...

IPS, inositol 1,4,5-trisphosphate; ER, endoplasmic reticulum; Hepes, 4-(2-hydroxyethyl)-l-piperazineeth- anesulfonic acid; EGTA, [ethylene bis(oxyethylenenitrilo)]tetraacetic GTP-yS, guanosine 5'-0-(3-thio)triphosphate; GppNHp, 5'-(&-y

Objective: Brain Computer Interfaces (BCIs) require substantial cognitive flexibility to optimize control performance across diverse settings. Remarkably, learning this is rapid, suggesting it might be mediated by neuroplasticity mechanisms operating on very short time scales. However, these remain far from understood. Here, we propose a meta plasticity model of BCI and skill consolidation at the single cell population levels comprised three elements: a) behavioral scale synaptic (BTSP), b)...

An intracellular (ATP + Mg2+)-dependent Ca2+ pumping mechanism has been identified and characterized within the cultured clonal neuroblastoma cell line N1E-115.Using suspensions treated with 0.005% saponin which selectively permeabilizes plasma membrane in 95-98% of cells, it was possible to show clearly that pump is non-plasma origin therefore can be compared directly Caz+ detail synaptosomal ves-

Abstract In this study, we characterized the effect of baicalein on regulation keratinocyte differentiation and proliferation, which are abnormal in atopic dermatitis or psoriasis. Treatment HaCaT keratinocytes with 10 μ m slightly inhibited cell growth, caused morphological increased expression keratins 1 (K1/K10) without affecting ROS generation, cytochrome c release apoptosis. Baicalein treatment growth arrest G 0 /G phase also induced Ca 2+ influx via TRPV 4 receptor activation....

In cultured porcine aortic smooth muscle cells, sphingosylphosphorylcholine (SPC), ATP, or bradykinin (BK) induced a rapid dose-dependent increase in the cytosolic Ca 2+ concentration ([Ca ] i ) and also stimulated inositol 1,4,5-trisphosphate (IP 3 generation. Pretreatment of cells with pertussis toxin blocked SPC-induced IP generation [Ca but had no effect on action ATP BK. addition, SPC mitogen-activated protein kinase (MAPK) increased DNA synthesis, whereas neither nor BK produced such...

In cultured rat cerebellar granule cells, glutamate or N-methyl-d-aspartate (NMDA) activation of the NMDA receptor caused a sustained increase in cytosolic Ca(2+) levels ([Ca(2+)](i)), reactive oxygen species (ROS) generation, and cell death (respective EC(50) values for were 12, 30, 38 microM) but no caspase-3 activity. Removal extracellular blocked all three glutamate-induced effects, whereas pretreatment with an ROS scavenger inhibited had effect on [Ca(2+)](i) increase. This indicates...

Baicalein is a flavonoid inhibitor of 12-lipoxygenase. Here, we investigated its effect on hydrogen peroxide-induced damage to NG108-15 cells. Hydrogen peroxide activated the mitochondrial apoptotic pathway, decreased Nrf2 expression, increased reactive oxygen species (ROS) levels, reduced viability, and cell death after 2-24 h treatment Co-treatment with baicalein completely suppressed activation pathway by upregulating expression reducing ROS stress partially inhibited effects viability...

Increased matrix metalloproteinase 1 (MMP-1) expression is a feature of photo-aged skin. We investigated the effects baicalein and sulphoraphane on ultraviolet B (UVB) irradiation-induced MMP-1 apoptosis using human dermal fibroblasts. UVB irradiation not only increased expression, but also caused apoptosis. Both protected cells from apoptosis, inhibited expression. activated 12-lipoxygenase, its product, 12-hydroxyeicosatetraenoic acid, TRPV1 channels. The resulting Ca2+ increase was...

Mesenchymal stromal/stem cells (MSCs) are widely distributed in different tissues such as bone marrow, adipose tissues, peripheral blood, umbilical cord and amnionic fluid. Recently, MSC-like were also found to exist rat olfactory bulb capable of inducing differentiation into mesenchymal lineages - osteocytes, chondrocytes adipocytes. However, whether these can differentiate myocardial is not known. In this study, we examined bulb-derived MSCs could vitro. Fibroblast-like isolated from the...

A sustained increase in the cytosolic Ca 2+ concentration ([Ca ] i ) can cause cell death. In this study, we found that, cultured porcine aortic smooth muscle cells, endoplasmic reticulum (ER) stress, triggered by depletion of stores thapsigargin (TG), induced an [Ca and However, TG-induced death was not related to but mediated targeting activated Bax mitochondria opening mitochondrial permeability transition pores (PTPs). Once PTPs had opened, several events, including collapse membrane...

To clarify the involvement of autophagy in neuronal differentiation, effect rapamycin, an mTOR complex inhibitor, on dibutyryl cAMP (dbcAMP)-induced differentiation NG108-15 cells was examined. Treatment with 1 mM dbcAMP resulted induction including neurite outgrowth and varicosity formation, enhanced voltage-sensitive Ca2+ channel activity expression microtubule-associated protein 2, these effects involved phosphorylation cAMP-response element binding (CREB) extracellular signal regulated...

// Hsin-Ying Clair Chiou 1 , Wen-Kuo Lai 2 Li-Chun Huang Shih-Ming Sheau-Huei Chueh Hsin-I Ma and Dueng-Yuan Hueng 1,2 Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C. Biochemistry, Correspondence to: Hueng, email: Keywords : meningioma, tumor stem-like cells, radiosensitization, valproic acid, Oct4 Received January 05, 2015 Accepted March Published 29, Abstract Although meningioma cells have been isolated...

In rat cerebellar astrocytes, intracellular Ca 2+ store depletion by receptor agonists or sarco(endo)plasmic reticulum ATPase inhibitors induced a transient increase in the concentration ([Ca ] i ) absence of extracellular and sustained its presence. After 10 min treatment with thapsigargin, [Ca was unaffected removal but fell rapidly to basal level when removed, suggesting involvement capacitative entry (CCE); this effect not seen until cells had been exposed thapsigargin for at least 2...

The pathology of Parkinson's disease (PD) results mainly from nigrostriatal pathway damage. Unfortunately, commonly used PD therapies do not repair the disconnected circuitry. It has been reported that using kainic acid (KA, an excitatory amino acid) in bridging transplantation may be useful to generate artificial tract and reconstruct 6-hydroxydopamine (6-OHDA) lesioned rats. In this study, we KA a co-graft rat olfactory ensheathing cells (OECs) E14 embryonic ventral mesencephalic (VM)...

Leucine-enkephalin (Leu-EK) dose-dependently elicited an increase in cytosolic Ca2+ concentration ([Ca2+]i) with EC50 of 1.2 microM via the phosphoinositide cascade NG108-15 cells. Chronic treatment cells [D-Ala2,D-Leu5]enkephalin caused time-dependent homologous desensitization. In presence extracellular Ca2+, ATP as well bradykinin stimulated significantly higher increases inositol 1,4,5-trisphosphate (IP3) generation than did Leu-EK; however, magnitude intracellular pools increased after...