A5047542675- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- HER2/EGFR in Cancer Research
- Porphyrin and Phthalocyanine Chemistry
- CAR-T cell therapy research
- Photodynamic Therapy Research Studies
- Synthesis and biological activity
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Optical Imaging and Spectroscopy Techniques
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- bioluminescence and chemiluminescence research
- Cellular transport and secretion
- Click Chemistry and Applications
- Photoacoustic and Ultrasonic Imaging
- Nanoparticle-Based Drug Delivery
- Ultrasound and Hyperthermia Applications
- Radiation Therapy and Dosimetry
- Synthesis of β-Lactam Compounds
University of California, Los Angeles
2014-2025
Cedars-Sinai Medical Center
2014-2023
Institute of Biomedical Science
2019-2022
California Institute of Technology
2008-2016
Technion – Israel Institute of Technology
2008-2016
Children's Hospital of Los Angeles
2016
University of Southern California
1997-2011
Fundación Medina
2007
LAC+USC Medical Center
2006
Southern California University for Professional Studies
1997
Sulfonated gallium(III) corroles are intensely fluorescent macrocyclic compounds that spontaneously assemble with carrier proteins to undergo cell entry. We report in vivo imaging and therapeutic efficacy of a tumor-targeted corrole noncovalently assembled heregulin-modified protein directed at the human epidermal growth factor receptor (HER). Systemic delivery this protein-corrole complex results tumor accumulation, which can be visualized owing red fluorescence. Targeted leads death while...
Idiopathic pulmonary fibrosis (IPF) is a chronic and fatal lung disease. A maladaptive epithelium due to injury prominent feature contributor pathogenic cellular communication in IPF. Recent data highlight the concept of "reprogrammed" as critical development fibrosis. Extracellular vesicles (EVs) are potent mediator crosstalk, recent evidence supports their role pathologies such Here, we demonstrate that syndecan-1 overexpressed by lungs IPF patients murine models after bleomycin injury....
In view of recent serious adverse events and advances in gene therapy technologies, the use regulatable expression systems is becoming recognized as indispensable adjuncts to successful clinical therapy. present work we optimized high-capacity adenoviral (HC-Ad) vectors encoding novel tetracycline-dependent (TetOn)-regulatory elements for efficient rat brain vivo. We constructed two HC-Ad beta-galactosidase (beta-gal) driven by a TetOn system containing rtTAS(s)M2 transactivator tTS(Kid)...
HerGa is a self-assembled tumor-targeted particle that bears both tumor detection and elimination activities in single, two-component complex (Agadjanian et al. Proc. Natl. Acad. Sci. U.S.A.2009, 106, 6105-6110). Given its multifunctionality, (composed of the fluorescent cytotoxic corrole macrocycle, S2Ga, noncovalently bound to cell penetration protein, HerPBK10) has potential for high clinical impact, but mechanism killing remains be elucidated, hence focus present study. Here we show...
Glioblastoma multiforme (GBM) is the most common subtype of primary malignant brain tumor. Although serotype 5 adenoviral vectors (Ads) have been used successfully in clinical trials for GBM, capacity Ads to infect human glioma cells and expression receptors GBM challenged. In this report, we studied three molecules that shown mediate entry into cells, i.e., coxsackie adenovirus receptor (CAR), integrin αvβ3 (INT), major histocompatibility complex class I (MHCI), rodent cell lines...
We optimized our previously reported proline-based STAT3 inhibitors into an exciting new series of (R)-azetidine-2-carboxamide analogues that have sub-micromolar potencies. 5a, 5o, and 8i STAT3-inhibitory potencies (IC50) 0.55, 0.38, 0.34 μM, respectively, compared to greater than 18 μM against STAT1 or STAT5 activity. Further modifications derived analogues, including 7e, 7f, 7g, 9k, addressed cell membrane permeability other physicochemical issues. Isothermal titration calorimetry analysis...
Signal transducer and activator of transcription (Stat)3 is a valid anticancer therapeutic target. We have discovered highly potent chemotype that amplifies the Stat3-inhibitory activity lead compounds to levels previously unseen. The azetidine-based compounds, including H172 (9f) H182, irreversibly bind Stat3 selectively inhibit (IC
This study presents a new multimodal imaging approach that includes high-frequency ultrasound, fluorescence intensity, confocal, and spectral to improve the preclinical evaluation of therapeutics in vivo. Here we use this assess vivo therapeutic efficacy novel chemotherapy construct, HerDox during after treatment. is comprised doxorubicin non-covalently assembled viral-like particle targeted HER2+ tumor cells, causing cell death at over 10-fold lower dose compared untargeted drug, while...
Triple-negative breast cancer (TNBC) has a high propensity for organ-specific metastasis. However, the underlying mechanisms are not well understood. Here we show that primary TNBC tumor-derived C-X-C motif chemokines 1/2/8 (CXCL1/2/8) stimulate lung-resident fibroblasts to produce C-C 2/7 (CCL2/7), which, in turn, activate cholesterol synthesis lung-colonizing cells and induce angiogenesis at lung metastatic sites. Inhibiting tumor by pulmonary administration of simvastatin-carrying...
We recently reported that a targeted, brightly fluorescent gallium corrole (HerGa) is highly effective for breast tumor detection and treatment. Unlike structurally similar porphryins, HerGa exhibits tumor-targeted toxicity without the need photoexcitation. have now examined whether photoexcitation further modulates toxicity, using multimode optical imaging of live cells, including two-photon excited fluorescence, differential interference contrast (DIC), spectral, lifetime imaging. Using...
We report fast, non-scanning, wide-field two-photon fluorescence excitation with spectral and lifetime detection for in vivo biomedical applications. determined the optical characteristics of technique, developed a Gaussian flat-field correction method to reduce artifacts resulting from non-uniform such that contrast is enhanced, showed it can be used ex cellular-level imaging. Two applications were demonstrated: (i) measurements beta-amyloid plaques retinas transgenic mice, (ii) imaging...
ABSTRACT The established mechanism for infection of most cells with adenovirus serotype 5 (Ad5) involves fiber capsid protein binding to coxsackievirus-adenovirus receptor (CAR) at the cell surface, followed by penton base α v integrins, which triggers clathrin-mediated endocytosis virus. Here we determined identity proteins responsible mediating Ad5 entry into acinar epithelial lacrimal gland. transduction primary rabbit was inhibited excess or knob (terminal region fiber) but not base....