A5008049865- Wnt/β-catenin signaling in development and cancer
- Hair Growth and Disorders
- Skin and Cellular Biology Research
- dental development and anomalies
- Dermatology and Skin Diseases
- Telomeres, Telomerase, and Senescence
- Autophagy in Disease and Therapy
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Exercise and Physiological Responses
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Cell death mechanisms and regulation
- Circadian rhythm and melatonin
- Immunotherapy and Immune Responses
- NF-κB Signaling Pathways
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Sympathectomy and Hyperhidrosis Treatments
- Neuropeptides and Animal Physiology
- Cancer and Skin Lesions
- Bacteriophages and microbial interactions
- Ion Channels and Receptors
- Neuroinflammation and Neurodegeneration Mechanisms
ZheJiang Academy of Agricultural Sciences
2025
Zhejiang A & F University
2025
National Institute on Aging
2014-2024
National Institutes of Health
2015-2024
Institute on Aging
2011-2024
Institut thématique Génétique, génomique et bioinformatique
2022-2023
University of Baltimore
2011-2023
South China Agricultural University
2020
Chinese Academy of Sciences
2020
National Human Genome Research Institute
2019
Abstract Skeletal muscle aging is a major cause of disability and frailty in the elderly. The progressive impairment skeletal function with was recently linked to disequilibrium between damage repair. Macrophages participate tissue repair, first as pro‐inflammatory M1 subtype then anti‐inflammatory M2 subtype. However, information on presence macrophages still sporadic effect macrophage phenotype remains unknown. In this study, we sought characterize polarization status persons across wide...
Tissue-resident macrophages represent a group of highly responsive innate immune cells that acquire diverse functions by polarizing toward distinct subpopulations. The subpopulations reside in skeletal muscle (SKM) and their changes during aging are poorly characterized. By single-cell transcriptomic analysis with unsupervised clustering, we found 11 macrophage clusters male mouse SKM enriched gene expression programs linked to reparative, proinflammatory, phagocytic, proliferative,...
Cells responding to DNA damage implement complex adaptive programs that often culminate in one of two distinct outcomes: apoptosis or senescence. To systematically identify factors driving each response, we analyzed human IMR-90 fibroblasts exposed increasing doses the genotoxin etoposide and identified SRC as a key kinase contributing early this dichotomous decision. was activated by low but not high levels etoposide. With damage, SRC-mediated activation p38 critically promoted expression...
Epigenetic alterations are a key hallmark of aging but have been limitedly explored in tissues. Here, using naturally aged murine liver as model and extending to other quiescent tissues, we find that is driven by temporal chromatin promote refractory cellular state compromise identity. Using an integrated multi-omics approach the first direct visualization chromatin, globally, old cells show H3K27me3-driven broad heterochromatinization transcriptional suppression. At local level,...
Body temperature is maintained in a narrow range mammals, primarily controlled by sweating. In humans, the dynamic thermoregulatory organ, comprised of 2–4 million sweat glands distributed over body, can secrete up to 4 L per day, thereby making it possible withstand high temperatures and endure prolonged physical stress (e.g., long-distance running). The genetic basis for gland function, however, largely unknown. We find that forkhead transcription factor, FoxA1, required generate mouse...
To maintain body temperature, sweat glands develop from embryonic ectoderm by a poorly defined mechanism. We demonstrate temporal cascade of regulation during mouse gland formation. Sweat induction failed completely when canonical Wnt signaling was blocked in skin epithelium, and accompanied sharp downregulation downstream Wnt, Eda Shh pathway genes. The antagonist Dkk4 appeared to inhibit this induction: sharply downregulated β-catenin-ablated mice, indicating that it is induced...
Cellular senescence is characterized by cell cycle arrest, resistance to apoptosis, and a senescence-associated secretory phenotype (SASP) whereby cells secrete pro-inflammatory tissue-remodeling factors. Given that the SASP exacerbates age-associated pathologies, some aging interventions aim at selectively eliminating senescent cells. In this study, drug library screen uncovered TrkB (NTRK2) inhibitors capable of triggering apoptosis several senescent, but not proliferating, human Senescent...
Lipid metabolism serves as the primary energy source for organisms. Silkworm eggs spring use are divided into two types: autumn-produced next rearing (AS) and spring-produced (SS). Production practice revealed significant differences in hatching rates between these types of silkworm production strain QiufengA. In this study, we identified differentially expressed genes (DEGs) primarily enriched pathways. particular, PPARs involved regulation through lipid metabolism. Furthermore, both AS SS...
In most species, oocytes are arrested at the prophase or metaphase of meiosis I and require sperm-derived external stimuli to resume meiosis. Maturation-promoting factor (MPF) is an oocyte maturation composing catalytic subunit Cdc2 regulatory CycB that can restart stalled this study, we demonstrated MPF activity affected parthenogenesis induction in model lepidopteran insect Bombyx mori using activator inhibitor interference. We found upregulation significantly increased rate, whereas...
Cyclin-dependent protein kinase 2 (Cdk2), an important member of the serine/threonine-specific family, plays a critical regulatory role in biological processes. Previous studies have demonstrated that Cdk2 is involved arrest and resumption meiosis mammalian oocytes. In this study, we explored function through parthenogenetic lines (PLs) corresponding amphigonic (ALs) model lepidopteran insect silkworm, Bombyx mori L. Our findings revealed positive correlation between activity parthenogenesis...
Mutations in the EDA gene cause anhidrotic/hypohidrotic ectodermal dysplasia, a disorder characterized by defective formation of hair, sweat glands, and teeth humans mouse model, "Tabby" (Ta). The encodes ectodysplasin, TNF ligand family member that activates NF-kappaB-signaling pathway, but downstream targets mechanism skin appendage have been only partially analyzed. Comparative transcription profiling embryonic during hair follicle development WT Ta mice identified critical dysplasia...
Abstract Changes in the proteome of different human tissues with advancing age are poorly characterized. Here, we studied proteins present primary skin fibroblasts collected from 82 healthy individuals across a wide spectrum (22–89 years old) who participated GESTALT (Genetic and Epigenetic Signatures Translational Aging Laboratory Testing) study National Institute on Aging, NIH. Proteins were extracted lysed subjected to liquid chromatography‐mass spectrometry analysis, expression levels...
EDA splice isoforms EDA-A1 and EDA-A2 belong to the TNF ligand family regulate skin appendage formation by activating NF-kappa B- JNK- promoted transcription. To analyze their action further, we conditionally expressed as tetracycline ('Tet')-regulated transgenes in Tabby (EDA-negative) wild-type mice. Expression of only mEDA-A1 transgene had two types effects during embryogenesis: (1) determinative on sweat glands hair follicles. In mice, one type follicle ('guard hair') was restored,...
The mouse hair coat comprises protective "primary" and thermo-regulatory "secondary" hairs. Primary formation is ectodysplasin (Eda) dependent, but it has been puzzling that Tabby (Eda-/y) mice still make secondary hair. We report Dickkopf 4 (Dkk4), a Wnt antagonist, affects an auxiliary pathway for Eda-independent development of A Dkk4 transgene in wild-type had no effect on primary hair, hairs were severely malformed. action was further demonstrated when the introduced into mice: usual...