A5101501308- Research in Cotton Cultivation
- Gastric Cancer Management and Outcomes
- Helicobacter pylori-related gastroenterology studies
- Gastrointestinal disorders and treatments
- Eosinophilic Esophagitis
- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Plant and Fungal Interactions Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Veterinary medicine and infectious diseases
- Plant Virus Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Galectins and Cancer Biology
- Cancer Cells and Metastasis
- Cardiovascular Disease and Adiposity
- Lymphatic System and Diseases
- Lipid metabolism and disorders
- Trace Elements in Health
- Metastasis and carcinoma case studies
- Cancer-related gene regulation
- Atherosclerosis and Cardiovascular Diseases
- Advanced Proteomics Techniques and Applications
- Gastrointestinal Tumor Research and Treatment
- Glycosylation and Glycoproteins Research
- Cancer-related molecular mechanisms research
The Fourth People's Hospital
2023-2025
Hainan Medical University
2024
Hainan Provincial Hospital of Traditional Chinese Medicine
2024
Guangzhou University of Chinese Medicine
2024
Fourth People's Hospital of Shenzhen
2023-2024
Longgang Central Hospital
2023
Nanjing Agricultural University
2014-2021
Southern Medical University Shenzhen Hospital
2019-2021
People's Liberation Army No. 150 Hospital
2015
Chinese People's Liberation Army
2015
Two draft sequences of Gossypium hirsutum, the most widely cultivated cotton species, provide insights into genome structure, rearrangement, gene evolution and fiber biology. Upland is a model for polyploid crop domestication transgenic improvement. Here we sequenced allotetraploid hirsutum L. acc. TM-1 by integrating whole-genome shotgun reads, bacterial artificial chromosome (BAC)-end genotype-by-sequencing genetic maps. We assembled annotated 32,032 A-subgenome genes 34,402 D-subgenome...
Cotton has been cultivated and used to make fabrics for at least 7000 years. Two allotetraploid species of great commercial importance, Gossypium hirsutum barbadense, were domesticated after polyploidization are worldwide. Although the overall genetic diversity between these two studied with limited accessions, their population structure variations remain largely unknown. We resequence genomes 147 cotton including diverse wild relatives, landraces, modern cultivars, construct a comprehensive...
Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers difficult. The recent emergence genome complexity reduction technologies based on next-generation sequencing (NGS) platform has greatly expedited SNP in crops with highly repetitive and complex genomes. Here we applied restriction-site associated DNA (RAD) technology for de novo allotetraploid cotton. We identified 21,109 SNPs between two parents...
To investigate the occurrence and development of gastric mucosal atrophy due to Helicobacter pylori (Hp) infection accompanying histomorphological features.Detailed histological observations immunohistochemical examinations were conducted via 197 endoscopic biopsies submucosal dissection specimens atrophic lesions with Hp infection. Detailed observation was made columnar cells in proliferative region deep pit isthmus gland, as well upper part glandular cervix.The mucosa by firstly led...
AIM:To investigate human epidermal growth factor receptor 2 (HER2) amplification and protein expression in mixed gastric carcinoma. METHODS:Fluorescence situ hybridization immunohistochemistry were used to detect HER2 277 cases of carcinoma.Protein staining intensity was rate as 1+, 2+, or 3+. RESULTS:Of the cases, 114 (41.2%) expressed protein.HER2 3+ observed 28/277 (10.1%) 2+ 37/277 (13.4%) 1+ 49/277 (17.7%) cases.A 17% detected, which 25/28 (89.3%) group, 17/37 (45.9%) 5/49 (10.2%) 1+.Of...
Cotton fiber is an excellent model for studying plant cell elongation and wall biogenesis as well because they are highly polarized use conserved diffuse growth mechanism. Fiber strength important trait among cotton qualities due to ongoing changes in spinning technology. However, the molecular mechanism of forming obscure. Through map-based cloning, we identified gene GhUBX. Increasing its expression, transgenic was significantly enhanced compared receptor W0 helices number remarkably...
OBJECTIVE: This study aims to investigate the significance of combined detection HER2 gene amplification and chemosensitivity in gastric cancer. METHODS: Immunohistochemistry (IHC), fluorescence situ hybridization (FISH) reverse-transcription polymerase chain reaction (RT-PCR ) were used analyze expression protein, mRNA ERCC1, TUBB3 TYMS genes 135 cases carcinoma. RESULTS: The rate protein was 39.3% (53/135). Among these positive cases, patients with (3+) accounted for 9.6% (13/135), (2+)...
Objective. To explore the relationship between HER2 gene and PD-1/PD-L1 in gastric cancer its significance. Methods. Immunohistochemistry (IHC) fluorescence situ hybridization (FISH) were used to detect protein expression, amplification, expression 78 cases of cancer. Results. The rate was 43.6% (34/78), which 19.4% (14/78) 3+, 14.1% (11/78) 2+, 11.5% (9/78) 1+. results showed that 19.2% (15/78) samples had 3.8% (3/78) a HER2/CEP17 ratio <2.0, amplificationf copy/cell ≥6.0, as detected by...
Objective To explore the early onset, development and histological features of gastric signet-ring cell carcinoma (SRCC). Methods Three hundred sixty-two patients with differentiated adenocarcinoma cells were enrolled. Histomorphological immunohistochemical patterns specimens observed in detail. Results Infection mucosa, especially by Helicobacter pylori , can cause massive proliferation transformation deep foveola, isthmus gland, proliferative zone upper neck gland. Signet-ring-like...
Background The pathological diagnosis and follow-up analysis of gastric mucosal biopsy have been paid much attention, some scholars proposed the 12 kinds lesions accompanying diagnosis, which is great significance for treatment precision diseases, improvement early rate cancer, reduction missed misdiagnosis rate. Aim To perform a histopathological classification chronic atrophic gastritis (CAG). Methods A total 2248 CAG tissue samples were collected, data their clinical characteristics also...
Objective: To investigate the histopathological characteristics of Helicobacter pylori (Hp) infection in gastric mucosa process from occurrence to intraepithelial neoplasia. Methods: Specimens obtained endoscopic biopsy and submucosal dissection 2457 cases Hp were observed assessed detail using histology immunohistochemistry techniques. The condition was divided according mucosal damage caused by infection. immunophenotype each stage subsequently elucidated. Results: is initially implanted...
To investigate the histomorphological characteristics and pathological types of hyperproliferation gastric surface epithelial cells.Hematoxylin Eosin, Periodic acid-Schiff, immunohistochemical staining were performed on biopsy specimens obtained from 723 patients with cells and/or hyperplasia pits. Follow-up gastroscopic reexaminations 475 included. Improvement probability was analyzed using Kaplan-Meyer as well Cox proportional hazards models.Seven different histomorphologies...
The aim of this study was to investigate the clinicopathological features and differential diagnosis gastric pleomorphic giant cell carcinoma. Histopathology, immunohistochemistry, human epidermal growth factor receptor 2 (HER2) gene testing were conducted for seven cases In histomorphological terms, all involved carcinoma, accounting more than 10% entire tumor, with spindle cells mixed various structures adenocarcinoma high, intermediate, low differentiation. There large heterogeneity in...
To investigate the histomorphological characteristics of gastric mucosa and prognosis in patients with Helicobacter pylori infection.Progressive damage to was examined by immunohistochemistry 2294 H. infection follow-up information analyzed.H. initially colonized mucus layer covered epithelium, then selectively adhered destroyed surface cells causing intra-gastric extra-gastric lesions. Gastric mucosal induced divided into five stages according depth invasion degree lesion deterioration:...
Liquid chromatograph-mass spectrometer (LC/MS) based labeled with isobaric mass tags for relative and absolute quantitation (iTRAQ) analyses were performed to identify differentially expressed proteins from normal cancerous human colorectal tissues. Around 802 identified, 68 of which defined as proteins. Bioinformatics analysis indicated that these correlated several specific cellular processes pathways have relationships pathological changes cancer (CRC). EHD2 selected verify its expression...
Tumor spread is a complex biological process closely related to tumor growth, which regulated by many genes within the cell. Recent studies have re-vealed that nm23 intimately metastasis in its biochemical nature, structure and function regulating role of gene itself[1]. In this study, product expression was performed 97 cases gastric cancer observations were made on relationship hyperplasia lymphatic tissue.
To investigate the image computer analysis of abnormally proliferating transformed cells gastric mucosa and its clinical significance. The pathological pictures adenomatous polyp cells, altered tubular adenocarcinoma in stomach were assessed by method on a total 96 gastroscopic biopsy ESD resection specimens. data cytoplasmic area, nuclear nuclear-cytoplasmic ratio, factor N-heterotypic index polyps, abnormal proliferative transformation intramucosal collected, mean, standard deviation...
The purpose of this study was to explore the histopathological staging and differential diagnosis marginal zone lymphoma in gastric mucosa-associated lymphoid tissue (MALT lymphoma). We performed detailed histomorphology immunohistochemistry investigations as well genetic testing on endoscopic biopsy mucosal resection specimens from 18 patients with MALT lymphoma. found that typically begins a small, isolated area outside follicular mantle or proliferates multifocal, patchy manner, gradually...