A5072016469- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- DNA Repair Mechanisms
- Vascular Malformations Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Telomeres, Telomerase, and Senescence
- Intracerebral and Subarachnoid Hemorrhage Research
- Intracranial Aneurysms: Treatment and Complications
- Immune Cell Function and Interaction
- Immune responses and vaccinations
- Chromosomal and Genetic Variations
- Cell Image Analysis Techniques
- Protein Degradation and Inhibitors
- PARP inhibition in cancer therapy
- Single-cell and spatial transcriptomics
- Diabetes and associated disorders
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Acute Lymphoblastic Leukemia research
Stanford University
2023-2025
University of Chicago
2020-2023
Stratford University
2023
Aging is associated with increased variability and dysregulation of the immune system. We performed a system-level analysis serum cytokines in longitudinal cohort 133 healthy individuals over 9 y. found that cancer incidence major contributor to cytokine abundance variability. Circulating increase up 4 y before diagnosis subjects age 80 also analyzed expression 10 types early-stage cancers from The Cancer Genome Atlas. similar set upregulated tumor tissues, specifically after Similarly,...
Understanding the mechanisms promoting chromosomal translocations of rearranging receptor loci in leukemia and lymphoma remains incomplete. Here we show that leukemias induced by aberrant activation β-catenin thymocytes, which bear recurrent Tcra/Myc-Pvt1 translocations, depend on Tcf-1. The DNA double strand breaks (DSBs) Tcra site translocation are Rag-generated, whereas Myc-Pvt1 DSBs not. Aberrantly activated redirects Tcf-1 binding to novel sites alter chromatin accessibility...
In human lupus nephritis, tubulointerstitial inflammation (TII) is associated with in situ expansion of B cells expressing anti-vimentin antibodies (AVAs). The mechanism by which AVAs are selected unclear. Herein, we demonstrate that AVA somatic hypermutation (SHM) and selection increase affinity for vimentin. Indeed, germline reversion several demonstrated higher can be from both low cell clones even those strongly reactive other autoantigens. While maturation, enzyme-linked immunosorbent...
Abstract All life forms undergo cell division and are dependent on faithful DNA replication to maintain the stability of their genomes. Both intrinsic extrinsic factors can stress process multiple checkpoint mechanisms have evolved ensure genome stability. Understanding these molecular is crucial for preventing treating genomic instability associated diseases including cancer. replicating fiber fluorography a powerful technique that directly visualizes cell’s response stress. Analysis...
Abstract “How is my immune system?” This a simple question, but there no metric to assess the overall performance of human system. Current clinical methods that evaluate person’s system only provide limited information, like cell counts or immunoglobulin levels, and fail capture global state. project aims develop Immunogram, generalized framework measure Immunogram Julia-based software links cells, proteins, other molecules in knowledge graph. delineates subgraph patterns describe states...
ABSTRACT In human lupus nephritis, tubulointerstitial inflammation (TII) is associated with in situ expansion of B cells expressing anti-vimentin antibodies (AVAs). The mechanism by which AVAs are selected unclear. Herein, we demonstrate that AVA somatic hypermutation and selection increase affinity for vimentin. However, enzyme-linked immunosorbent assays (ELISAs) suggested maturation might be a non-specific consequence increasing polyreactivity. Subsequent multi-color confocal microscopy...
Abstract Lymphocyte development consists of sequential and mutually exclusive cell states proliferative selection antigen receptor gene recombination 1 . Transitions between each state require large, coordinated changes in epigenetic landscapes transcriptional programs 2,3 How this occurs remains unclear. Herein, we demonstrate that small pre-B cells, the lineage stagespecific reader Bromodomain WD Repeating Containing Protein (BRWD1) 2,4 reorders three-dimensional chromatin topology to...