A5057595682- Trypanosoma species research and implications
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Research on Leishmaniasis Studies
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Zoonotic diseases and public health
- Hepatitis B Virus Studies
- Virology and Viral Diseases
- Mosquito-borne diseases and control
- Rabies epidemiology and control
- Vector-borne infectious diseases
- Insect symbiosis and bacterial influences
- Yersinia bacterium, plague, ectoparasites research
- Livestock and Poultry Management
- Lysosomal Storage Disorders Research
- Poxvirus research and outbreaks
- Viral gastroenteritis research and epidemiology
- Parasites and Host Interactions
- Bacillus and Francisella bacterial research
- Helminth infection and control
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Whipple's Disease and Interleukins
National Institute of Biomedical Research
2012-2024
Université Pédagogique Nationale
2012-2024
Pedagogical University
2023
University of Kinshasa
2012-2022
Instituut voor Tropische Geneeskunde
2006-2014
University of Pretoria
2008-2014
Southern African Centre for Infectious Disease Surveillance
2012
Central Veterinary Research Laboratory
2009
Onderstepoort Veterinary Academic Hospital
2009
The Egyptian fruit bat, Rousettus aegyptiacus, is currently regarded as a potential reservoir host for Marburg virus (MARV). However, the modes of transmission, level viral replication, tissue tropism and shedding pattern remains to be described. Captive-bred R. including adult males, females pups were exposed MARV by different inoculation routes. Blood, tissues, feces urine from 9 bats inoculated combination nasal oral routes all negative ELISA IgG antibody could not demonstrated up 21 days...
BackgroundBas-Congo virus (BASV), an emerging tibrovirus, was associated with outbreak of acute haemorrhagic fever in Mangala, Democratic Republic the Congo, 2009. In 2012, neutralising antibodies to BASV were detected lone survivor and one his close contacts. However, subsequent serological molecular surveys unsuccessful as neither nor its RNA detected. this study, we determined seroprevalence infection Mangala 13 years after initial outbreak.MethodsWe conducted a population-based survey...
Worldwide, the highest malaria mortality is due to Plasmodium falciparum infection. However, other species of (Plasmodium vivax, ovale, malariae, and knowlesi) can also cause malaria. Therefore, accurate identification crucial for patient management epidemiological surveillance. This study aimed determine different causing in children under 5 years old two provinces (Kinshasa North Kivu) Democratic Republic Congo (DRC). From October December 2015, a health-facility based cross-sectional was...
Tsetse-transmitted trypanosomosis (nagana) has been the cause of stock losses in recent past and still presents a major problem to livestock owners certain areas KwaZulu- Natal, South Africa. Over 10 000 cattle mortalities were reported 1990 nagana outbreak. Although information on distribution abundance tsetse flies Glossina brevipalpis austeni KwaZulu-Natal exists, data their vector competence are lacking. This study aimed determine rate natural Trypanosoma congolense infection by...
The recent large outbreaks of Ebola virus disease (EVD) in West Africa and the Democratic Republic Congo (DRC) have highlighted need for rapid diagnostic tests to control this disease. In study, we clinically evaluated a previously developed immunochromatography-based kit, QuickNaviTM-Ebola. During 2018 DRC, 928 blood samples from EVD-suspected cases were tested with QuickNaviTM-Ebola WHO-approved GeneXpert. sensitivity specificity QuickNaviTM-Ebola, estimated by comparing it...
Abstract Crimean–Congo hemorrhagic fever virus (CCHFV) causes a zoonotic disease, (CCHF) endemic in Africa, Asia, the Middle East, and Southeastern Europe. However, prevalence of CCHF is not monitored most countries due to limited availability diagnostic assays biosafety regulations required for handling infectious CCHFV. In this study, we established protocol purify recombinant CCHFV nucleoprotein (NP), which antigenically highly conserved among multiple lineages/clades CCHFVs investigated...
Trypanosoma congolense and vivax are major species that infect cattle in north-eastern KwaZulu-Natal (KZN), South Africa. Of the two genetically distinct types of T. congolense, Savannah Kilifi sub-groups, isolated from tsetse flies KZN, former is more prevalent thought to be responsible for African animal trypanosomosis outbreaks cattle. Furthermore, variation pathogenicity within sub-group ascribed strain differences seems related geographical locations. The objective present study was...
The latest outbreak of Ebola virus disease (EVD) in West Africa has highlighted the urgent need for development rapid and reliable diagnostic assays. We used monoclonal antibodies specific to ebolavirus nucleoprotein develop an immunochromatography (IC) assay (QuickNavi-Ebola) diagnosis EVD. IC was first evaluated with tissue culture supernatants infected Vero E6 cells found be capable detecting 103-104 focus-forming units/mL ebolaviruses. Using serum samples from experimentally nonhuman...
Rabies is one of the major public health problems mostly affecting developing countries in Africa and Asia where 99.9% all rabies related human deaths are recorded each year. In Democratic Republic Congo, repeated outbreaks have been reported. Despite this, there little reliable epidemiological data about country for development effective control strategies.A retrospective study was carried out Kinshasa Province during a period five years (2009-2013) to describe proportion rabid animals...
The last wild poliovirus (WPV) case in Africa was reported July 2014, thus underscoring the tremendous progress towards polio eradication worldwide. This study aimed to analyze results of a seven-year surveillance Acute Flaccid Paralysis (AFP) Democratic Republic Congo (DRC) and identify potential gaps that need be addressed.Epidemiological virological data obtained from AFP among cases less than 15 years January 2008 December 2014 DRC were retrospectively considered analyzed this study.Of...
Newcastle disease (ND) is a highly transmissible and devastating that affects poultry wild birds worldwide. Comprehensive knowledge regarding the characteristics epidemiological factors of ND virus (NDV) critical for control prevention ND. Effective vaccinations can prevent spread NDV in populations. For decades, Democratic Republic Congo (DRC) has reported impacts on commercial traditional farming systems. The reports were preliminary clinical observations, few cases confirmed laboratory....
Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is tick-borne zoonotic that causes in humans. The CCHFV nucleoprotein (NP) the antigen most used for serological screening of infection animals and To gain insights into antibody epitopes on NP molecule, we produced recombinant chimeric NPs between Nairobi sheep disease (NSDV), which another tested rabbit mouse antisera/immune ascites, anti-NP monoclonal antibodies, CCHFV-infected animal/human sera their reactivities to antigens....
The aim of this study was to assess the existence possible cross-protection between Trypanosoma congolense strains low and extreme virulence circulating in same trypanosomiasis focus. Groups six mice were infected using one three challenged with virulence. A group used as control for each strain results showed that developed high parasitaemia a significant drop PCV compared With an exception (strain F), survival time shorter subsequently These suggest area where trypanosomes various profiles...
Rabies is a preventable fatal disease that causes about 61,000 human deaths annually around the world, mostly in developing countries. In Africa, several studies have shown vaccination of pets effective controlling disease. An annual coverage 70% recommended by World Health Organization as control threshold. The rabies requires owned dogs. Identification factors determining dog owners' choice to vaccinate necessary for evidence-based policy-making. However, Democratic Republic Congo (DRC),...
Transmission experiments were conducted to compare the transmissibility of genetically different Trypanosoma congolense (Savannah subgroup) strains isolated from cattle in a trypanosomiasis endemic area eastern Zambia. A total 17 compared. Three extremely virulent with short pre-patent period, high parasitaemia and median survival time (between 5 9 days) mice. The remainder belonged moderate (6 strains) or low (8 virulence categories times between 10 30 days >30 days, respectively....
In order to verify whether the low impact on livestock production in endemic areas is related a number of trypanosome strains circulating livestock, 37 Trypanosoma congolense isolates collected from cattle 11 sites an trypanosomiasis area Eastern Zambia were characterised for genotype variability using modified amplified fragment length polymorphism technique (AFLP). Isolates further cloned evaluate occurrence mixed infections individuals. The results obtained revealed high diversity (94.6%)...
Background Spliced Leader (SL) trypanosome RNA is detectable only in the presence of live trypanosomes, abundant and Trypanozoon subgenus has a unique sequence. As previously shown blood from Guinean human African trypanosomiasis (HAT) patients, SL-RNA an accurate target for diagnosis. Detection cerebrospinal fluid (CSF) never been attempted. In large group Congolese gambiense HAT present study aims i) to confirm sensitivity detection and; ii) assess diagnostic performance compared CSF....