A5055952862- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Genetics, Aging, and Longevity in Model Organisms
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Cephalopods and Marine Biology
- Biotin and Related Studies
- Electron and X-Ray Spectroscopy Techniques
- Neuroscience and Neuropharmacology Research
- Physiological and biochemical adaptations
- Iron and Steelmaking Processes
- Functional Brain Connectivity Studies
- Machine Learning in Materials Science
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Mineral Processing and Grinding
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Transportation Planning and Optimization
- Neural dynamics and brain function
- Sharing Economy and Platforms
- Image Processing Techniques and Applications
- Ichthyology and Marine Biology
Harvard University
1996-2025
Northeastern University
2023
Dalian University of Technology
2022-2023
Harvard University Press
1994-2021
Tsinghua University
2014
Breast Center
2008
Anhui Provincial Meteorological Bureau
2002
Joslin Diabetes Center
1994
University of Pennsylvania
1994
University of Georgia
1994
Abstract We acquired a rapidly preserved human surgical sample from the temporal lobe of cerebral cortex. stained 1 mm 3 volume with heavy metals, embedded it in resin, cut more than 5000 slices at ∼30 nm and imaged these sections using high-speed multibeam scanning electron microscope. used computational methods to render three-dimensional structure containing 57,216 cells, hundreds millions neurites 133.7 million synaptic connections. The 1.4 petabyte microscopy volume, segmented cell...
To fully understand how the human brain works, knowledge of its structure at high resolution is needed. Presented here a computationally intensive reconstruction ultrastructure cubic millimeter temporal cortex that was surgically removed to gain access an underlying epileptic focus. It contains about 57,000 cells, 230 millimeters blood vessels, and 150 million synapses comprises 1.4 petabytes. Our analysis showed glia outnumber neurons 2:1, oligodendrocytes were most common cell, deep layer...
Here, we present the first analysis of connectome a small volume
Phosphorylation of glucose to 6-phosphate by glucokinase (GK; EC 2.7.1.2) serves as a glucose-sensing mechanism for regulating insulin secretion in beta cells. Recent findings heterozygous GK gene mutations patients with maturity-onset diabetes the young (MODY), form type II (non-insulin-dependent) characterized autosomal dominant inheritance, have raised possibility that decrease beta-cell activity may impair secretory response these cells glucose. To generate an animal model MODY we...
Abstract From birth to adulthood, an animal’s nervous system changes as its body grows and behaviours mature. The form extent of circuit remodelling across the connectome is unknown. We used serial-section electron microscopy reconstruct full brain eight isogenic C. elegans individuals postnatal stages learn how it with age. overall geometry preserved from adulthood. Upon this constant scaffold, substantial in chemical synaptic connectivity emerge. Comparing connectomes among reveals...
Accumulating information is a critical component of most circuit computations in the brain across species, yet its precise implementation at synaptic level remains poorly understood. Dissecting such neural circuits vertebrates requires knowledge functional properties and ability to directly correlate dynamics with underlying wiring diagram same animal. Here we combine calcium imaging ultrastructural reconstruction, using visual motion accumulation paradigm larval zebrafish. Using connectomic...
Abstract Mapping neuronal networks is a central focus in neuroscience. While volume electron microscopy (vEM) can reveal the fine structure of (connectomics), it does not provide molecular information to identify cell types or functions. We developed an approach that uses fluorescent single-chain variable fragments (scFvs) perform multiplexed detergent-free immunolabeling and volumetric-correlated-light-and-electron-microscopy on same sample. generated eight scFvs targeting brain markers....
Abstract Mapping the complete synaptic connectivity of a mammalian brain would be transformative, revealing pathways underlying perception, behavior, and memory. Serial section electron microscopy, via membrane staining using osmium tetroxide, is ideal for visualizing cells connections but, in whole samples, faces significant challenges related to chemical treatment volume changes. These issues can adversely affect both ultrastructural quality macroscopic tissue integrity. By leveraging...
Glucose-induced insulin release from pancreatic beta cells depends on the beta-cell metabolism of glucose, which generates intracellular signals for secretion. The glucose transporter isotype GLUT2 and phosphorylating enzyme glucokinase have both been implicated in coupling secretion to extracellular levels. Here we present evidence that a pronounced decrease has no immediate effect homeostasis. Analysis transgenic mice overexpressing human [Val12]HRAS oncoprotein under control promoter...
The present study evaluated the involvement of glucose transport and phosphorylation in glucose-stimulated insulin release from pancreatic islets. Using quantitative histochemical techniques, we investigated basal islet content, uptake situ during acute extreme experimental hyperglycemia, glucokinase activity several animal models diabetes obesity. content anaesthetized diabetic or obese rodents was either same higher than that their relevant controls. rate tissue these animals after an i.v....
Mapping neuronal networks that underlie behavior has become a central focus in neuroscience. While serial section electron microscopy (ssEM) can reveal the fine structure of (connectomics), it does not provide molecular information helps identify cell types or their functional properties. Volumetric correlated light and (vCLEM) combines ssEM volumetric fluorescence to incorporate labeling into datasets. We developed an approach uses small fluorescent single-chain variable fragment (scFv)...
Abstract The sense of smell is generated by electrical currents that are influenced the concentration ions in olfactory sensory neurons and mucus. In contrast to extensive morphological molecular characterization neurons, there has been little description cells control ion concentrations zebrafish system. Here, we report ultrastructural ionocytes. Transcriptome analysis suggests epithelium contains at least three different ionocyte types, which resemble Na + /K -ATPase-rich (NaR), /Cl -...
Connectomics provides essential nanometer-resolution, synapse-level maps of neural circuits to understand brain activity and behavior. However, few researchers have access the high-throughput electron microscopes necessary generate enough data for whole circuit or reconstruction. To date, machine-learning methods been used after collection images by microscopy (EM) accelerate improve neuronal segmentation, synapse reconstruction other analysis. With computational improvements in processing...
Hepatocellular carcinoma is a major health problem worldwide. Different treatment strategies have been developed to cope with this problem. Herbal medicine now widely studied in both Eastern and Western countries. In study, we used vitro vivo model illustrate the anti-tumor effect of product, CKBM, consisting herbal specially processed Saccharomyces cerevisiae. Dose-dependent anti-proliferation was observed on growth human hepatoma HepG2 cells after 48 hours incubation CKBM. At 50%...
Connectomics is a nascent neuroscience field to map and analyze neuronal networks. It provides new way investigate abnormalities in brain tissue, including models of Alzheimer's disease (AD). This age-related associated with alterations amyloid-β (Aβ) phosphorylated tau (pTau). These correlate AD's clinical manifestations, but causal links remain unclear. Therefore, studying these molecular within the context local glial milieu may provide insight into mechanisms. Volume electron microscopy...
Summary During development, animals can maintain behavioral output even as the underlying circuits structurally remodel. After hatching, C. elegans undergoes substantial motor neuron expansion and synapse re-wiring while animal continuously moves with an undulatory pattern. To understand how circuit transitions from its juvenile to mature configuration without disrupting functional output, we reconstructed by electron microscopy across larval development. We observed: 1) Embryonic neurons...
Blast furnace ironmaking is one of the most serious carbon dioxide emission processes. To reduce energy consumption and CO2 emissions, fluidized bed technology with hydrogen as a reducing agent has attracted more attention. An inclined agitator was added to reactor address sticking issue in conventional process. In this research, numerical simulation used examine impacts gas composition agitation speed on gas-solid fluidization quality cold iron ore powder an agitator. The results indicate...