A5013933528- DNA Repair Mechanisms
- Acute Myeloid Leukemia Research
- Blood groups and transfusion
- Prenatal Screening and Diagnostics
- Mast cells and histamine
- Chronic Myeloid Leukemia Treatments
- Eosinophilic Disorders and Syndromes
- Effects of Radiation Exposure
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Lymphoma Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Childhood Cancer Survivors' Quality of Life
- Carcinogens and Genotoxicity Assessment
- Radiation Dose and Imaging
- Parvovirus B19 Infection Studies
- PARP inhibition in cancer therapy
- Blood disorders and treatments
- Erythrocyte Function and Pathophysiology
- Multiple Myeloma Research and Treatments
- Cancer Risks and Factors
- Asthma and respiratory diseases
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- Advanced Radiotherapy Techniques
Heidelberg University
2013-2022
University Medical Centre Mannheim
2016-2022
University Hospital Heidelberg
2013-2022
Medizinische Fakultät Mannheim
2007-2021
Ludwig-Maximilians-Universität München
2010-2011
Munich Leukemia Laboratory (Germany)
2010
Helmholtz Zentrum München
2010
LMU Klinikum
2009
Agaplesion Markus Hospital
2006
Justus-Liebig-Universität Gießen
2006
The clinical behavior of systemic mastocytosis (SM) is strongly associated with activating mutations in KIT (D816V >80% cases), the severity phenotype influenced by additional somatic mutations, for example, SRSF2, ASXL1, or RUNX1. Complex molecular profiles are frequently presence an hematologic neoplasm (AHN) and unfavorable outcome. However, little known about incidence prognostic impact cytogenetic aberrations. We analyzed characteristics 109 patients (KIT D816V+, n = 102, 94%) indolent...
Abstract The aim of this study was to systematically evaluate the cumulative radiation exposure and associated lifetime-cancer-risk from diagnostic imaging in patients with Hodgkin-lymphoma-(HL) or diffuse-large-B-cell-lymphoma (DLBCL). 99 consecutive (53-males) diagnosed HL DLBCL were included followed. Based on reports, organ effective-doses-(ED) calculated individually for each patient excess lifetime risks estimated. average ED first year after diagnosis significantly different men (59 ±...
DNA damage and alterations in the response (DDR) are critical sources of genetic instability that might be involved BCR-ABL1 kinase-mediated blastic transformation chronic myeloid leukemia (CML). Here, increased is detected by γH2AX foci analysis peripheral blood mononuclear cells (PBMCs) de novo untreated phase (CP)-CML patients (n = 5; 2.5 per PBMC ± 0.5) blast (BP)-CML 3; 4.4 0.7) as well CP-CML with loss major molecular (MMR) 1.8 0.4) when compared to healthy donors 8; 1.0 0.1) deep or...
DNA double-strand breaks (DSB) are serious lesions. Analysis of the formation and repair DSB is relevant in a broad spectrum research areas including genome integrity, genotoxicity, radiation biology, aging, cancer, drug development. In response to DSB, histone H2AX phosphorylated at Serine 139 region several megabase pairs forming discrete nuclear foci detectable by immunofluorescence microscopy. addition, 53BP1 (p53 binding protein 1) another important DSB-responsive promoting...
Background and Objectives Identification of fetal DNA in maternal plasma may allow genetic analysis without the use invasive techniques. The aim this study was to extract from plasma, identify material through presence SRY or RHD gene sequences assess reliability these results. Materials Methods A polymerase chain reaction (PCR) method a commercial kit used with primers for exon 10 sequence. Results Multiple samples were collected 60 women who evaluable either , both, fetally derived...
A sensitive test for the presence of D-positive fetal red blood cells (RBCs) in maternal circulation D-negative women has been developed. It was used to investigate possibility that occasional failure preventing alloimmunization might be due administration inadequate amounts prophylactic anti-D Rh immune globulin. The standard dose Australia contains 125 microg antibody, and can suppress immunization by an estimated 6 mL packed RBCs. fetomaternal hemorrhage (FMH) this volume is detectable as...
Abstract COVID-19 in patients with hematological diseases is associated a high mortality. Moreover, preventive vaccination demonstrated reduced efficacy and the knowledge on influencing factors limited. In this single-center study, antibody levels of SARS-CoV-2 spike protein were measured ≥ 2 weeks after 2nd concentration 0.8 U/mL considered positive. Between July October 2021, total 373 (median age 64 years, 44% women) myeloid neoplasms ( n = 214, 57%), lymphoid 124, 33%), other 35, 10%),...
Abstract Background and Objectives : The Kleihauer slide test is in general use to screen obstetric patients for possible fetomaternal haemorrhage. Since 1993, Rh(D)‐negative have been tested our laboratory by a flow‐cytometric method detecting Rh(D)‐positive fetal cells, which offers improved sensitivity accuracy. We report another of broader application quantitates cells according haemoglobin F (HbF) content. Materials Methods red are fixed with glutaraldehyde permeabilized exposure Triton...
In systemic mastocytosis (SM), qualitative and serial quantitative assessment of the KIT D816V mutation is diagnostic prognostic relevance. We investigated peripheral blood bone marrow samples 161 patients (indolent SM (ISM), n = 40; advanced SM, AdvSM, 121) at referral during follow-up for variant allele frequency (VAF) DNA-level expressed burden (EAB) RNA-level. A round robin test with four participating laboratories revealed an excellent correlation (r > 0.99, R2 0.98) between three...
Malignant hematopoietic cells of myelodysplastic syndromes (MDS)/chronic myelomonocytic leukemias (CMML) and acute myeloid (AML) may be vulnerable to inhibition poly(ADP ribose) polymerase 1/2 (PARP1/2) apurinic/apyrimidinic endonuclease 1 (APE1). PARP1/2 APE1 are critical enzymes involved in single-strand break repair base excision repair, respectively. Here, we investigated the cytotoxic efficacy talazoparib inhibitor III, inhibitors APE1, primary CD34+ MDS/CMML cell samples (n = 8; 4 MDS...
Accumulation of DNA damage and alteration the response (DDR) are critical features genetic instability that is presumed to be implicated in pathogenesis monoclonal B-cell lymphocytosis (MBL) chronic lymphocytic leukemia (CLL). Here, we show increased numbers γH2AX foci, a marker double-strand breaks (DSB), CD19+ cells CLL patients as compared MBL healthy individuals. Furthermore, numerous γH2AX/53BP1 foci suggest activation error-prone non-homologous end-joining repair mechanisms. Signatures...
Background: Small cell lung cancer (SCLC) results in death within 1–2 months if left untreated. Although therapeutic standards only comprise first- and second-line chemotherapy due to poor prognosis, a subset of patients may warrant trial further chemotherapy, as demonstrated the following case. Case Report: We present 52-year-old man with confirmed anaplastic SCLC who survived 10 years while receiving 9 lines including high-dose chemotherapy. Thoracic radiation, afterloading radionuclide...
Methods are described for the identification and quantitation of mixed red cell populations using flow cytometry. Antibodies specific a wide range blood group antigens have been used examples given in which these analyses proved to be clinical use. These include monitoring erythropoiesis following engraftment allogeneic bone marrow transplant recipients detection chimaeric states months or years after transplantation. The techniques involved fast, simple inexpensive.
Genotoxic bystander signals released from irradiated human mesenchymal stromal cells (MSC) may induce radiation-induced effects (RIBEs) in hematopoietic stem and progenitor (HSPC), potentially causing leukemic transformation. Although the source of is evident, identification characterization these challenging. Here, RIBEs were analyzed CD34+ cultured distinct molecular size fractions medium, conditioned by 2 Gy MSC. Specifically, γH2AX foci (as a marker DNA double-strand breaks) chromosomal...
Biallelic mutations in BRCA2/FANCD1 were recently recognized as a rare cause of Fanconi anemia (FA). Using immunodetection with an antiserum directed against the carboxyterminus BRCA2 protein, we screened 38 lymphoid cell lines from FA patients whom could not previously assign, via retroviral complementation analysis, to any six known groups (FA-A, -C, -D2, -E, -F, or -G). Three these lacked 380-kDa signal on immunoblots. DNA sequencing showed biallelic compound and truncating two...