A5075421950- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Genomics and Rare Diseases
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Neuroendocrine Tumor Research Advances
- Cancer Genomics and Diagnostics
- Connective tissue disorders research
- Lung Cancer Research Studies
- Bone and Dental Protein Studies
- Genomics and Phylogenetic Studies
- Neuroblastoma Research and Treatments
- Cancer Research and Treatments
- Lung Cancer Treatments and Mutations
- Cell Adhesion Molecules Research
- Microbial Community Ecology and Physiology
- Genetic Associations and Epidemiology
- RNA and protein synthesis mechanisms
- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- Esophageal Cancer Research and Treatment
First Affiliated Hospital of Zhengzhou University
2015-2025
Chinese PLA General Hospital
2024
BGI Group (China)
2016-2024
Technical University of Denmark
2022-2024
BGI Genomics
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Shandong University
2024
Tianjin Medical University
2016-2023
China North Industries Group Corporation (China)
2020-2021
Jilin Academy of Traditional Chinese Medicine
2020-2021
This study aims to clarify the underlying mechanism for tumor suppressive function of lnc TUSC7 in chemotherapy resistance esophageal squamous cell carcinoma (ESCC).TUSC7, miR-224 and DESC1 expressions ESCC tissues cells were detected by qRT-PCR. Protein level DESC1, EGFR p-AKT observed Western blot. Overall survival was calculated using Kaplan-Meier method. Dual-luciferase reporter gene assay RIP used comfirm binding miR-224, DESC1. Cell proliferation, apoptosis, colony formation MTT, Flow...
Abstract Background Due to its reduced cost and incomparable advantages, WGS is likely lead changes in clinical diagnosis of rare undiagnosed diseases. However, the sensitivity breadth coverage as a diagnostic test for genetic disorders has not been fully evaluated. Methods Here, performance NA12878, YH cell line, Chinese trios were measured by assessing their sensitivity, PPV, depth using MGISEQ-2000. We also compared WES NA12878. The PPV tested family-based trio design trios. further...
Abstract Background Increasing studies focused on the regulatory roles of circular RNAs (circRNAs) in diverse cancers. This study was to evaluate function and mechanism circRNA Scm-like with four malignant brain tumor domains 2 (circ-SFMBT2) esophageal cancer (EC). Methods The circ-SFMBT2, microRNA-107 (miR-107) solute-linked carrier family A1 member 5 (SLC1A5) levels were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation evaluated 3-(4,...
Patients with biliary tract cancer (BTC) were associated poor prognosis and limited therapeutic options after first-line therapy currently. In this study, we sought to evaluate the feasibility tolerability of sintilimab plus anlotinib as second-line treatment for patients advanced BTC. Eligible had histologically confirmed locally unresectable or metastatic BTC failed recruited. The primary endpoint was overall survival (OS). Simultaneously, association between clinical outcomes genomic...
In recent years, the incidence of patients with colorectal neuroendocrine neoplasms (CRNENs) has been continuously increasing. When diagnosed, most have distant metastases. Liver metastasis (LM) is common type metastasis, and prognosis poor once it occurs. However, there still a lack large studies on risk LM in CRNENs. This study aims to identify factors related develop predictive model accordingly. this study, Surveillance, Epidemiology, End Results (SEER) database was used collect clinical...
The genus Paenibacillus is a prolific producer of secondary metabolites with diverse ecological and industrial applications. However, comprehensive overview the biosynthetic gene cluster (BGC) diversity distribution throughout has been limited. Here, we performed large-scale genome mining on 284 high-quality genomes generated non-redundant dataset 126 representative to explore potential this genus. A total 3,273 BGCs were identified from that clustered into 1,013 families (GCFs), 98.7%...
BACKGROUND MicroRNAs (miRNAs) are a class of small non-coding RNAs that strongly involved in various types carcinogenesis, including hepatocellular carcinoma (HCC). This study aimed to clarify whether miR-4417 promotes HCC growth by targeting TRIM35 and regulating PKM2 phosphorylation. MATERIAL AND METHODS Online software, TargetScan miRanda, was used predict the potential target miR-4417. Real-Time PCR (qRT-PCR) Western blot assays were performed detect expression levels mRNA protein,...
Abstract The underlying mechanisms of breast cancer cells metastasizing to distant sites are complex and multifactorial. Bone sialoprotein ( BSP ) αvβ3 integrin were reported promote the metastatic progress cells, particularly metastasis bone. Most theories presume that promotes by binding integrin. Interestingly, we found decreased in silenced i), which have weak ability form bone metastases. However, relevance their expression primary tumor way they participate not clear. In this study,...
Autosomal dominant non-syndromic hearing loss (ADNSHL) is highly heterogeneous, among them, KCNQ4 one of the most frequent disease-causing genes. More than twenty mutations have been reported, but none them were detected in Chinese mainland families. In this study, we identified a novel mutation five generation family with 84 members and known six 66 members. Mutation screening 30 genes for ADNSHL was performed probands from thirty large families by targeted region capture high-throughput...
Invasion and metastasis is the major cause of tumor recurrence, difficulty for cure low survival rate. Excavating key transcription factors, which can regulate invasion metastasis, are crucial to development therapeutic strategies cancers. PU.1 a master hematopoietic factor vital regulator in life. Here, we report that, compared adjacent non-cancerous tissues, expression mRNA metastatic hepatocellular carcinoma (HCC), but not primary HCC, was significantly down-regulated. In addition, levels...
Bacillus species are ubiquitous in nature and have tremendous application potential agriculture, medicine, industry. However, the individual of this genus vary widely both ecological niches functional phenotypes, which, hence, requires accurate classification these bacteria when selecting them for specific purposes. Although analysis 16S rRNA gene has been used to disseminate taxonomy most bacterial species, fails proper species. To circumvent restriction, we designed novel primers optimized...
Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant cancers with high mortality around world. However, regulatory mechanism ESCC carcinogenesis not completely known. Here we demonstrate novel role miR-202 in regulating apoptosis. The analysis data obtained from GEO database showed that expression aberrantly decreased tumor tissue patients and cultured lines. After transfection mimic or inhibitor, apoptotic capacity cells was significantly increased by overexpression...
Abstract T cells expressing programmed cell death 1 (PD-1) in the peripheral blood (PB) of patients with tumors possess therapeutic potential; however, immunosuppressive, PD-1–triggered signaling pathway and limited proliferative capacity PD-1+ present challenges to their application. Here, we observed no discernible distinction between PD-1− terms clonal overlap. However, CD8+PD-1+ from PB tumor tissues exhibited tighter clustering based on clone size. Single-cell RNA sequencing analysis...