Abstract Background Endothelial damage is an initial step of macro- and micro-vasculature dysfunctions in diabetic patients, accounting for a high incidence vascular complications, such as atherosclerosis, nephropathy, retinopathy, neuropathy. However, clinic lacks effective therapeutics targeting complications. In field regenerative medicine, mesenchymal stem cells, human umbilical cord-derived MSCs (hucMSCs), have great potential treating tissue damage. Methods To determine whether hucMSCs...

Acute liver injury (ALI) is a poor prognosis and high mortality complication of sepsis. Paeoniflorin (PF) has remarkable anti-inflammatory effects in different disease models. Here, we explored the protective effect underlying molecular mechanisms PF against lipopolysaccharide (LPS)-induced ALI. Sprague-Dawley rats received intraperitoneal (i.p.) injection for 7 days, 1 h after last administration, were injected i.p. 10 mg/kg LPS. improved structure function, reduced hepatic reactive oxygen...

Diabetic cardiomyopathy (DCM), which is associated with many pathological processes, commonly occurs when advanced glycation end products (AGEs) are present. β-carotene (BC) a well-known vitamin A precursor that found in fruits and vegetables. BC can reduce the risk of cancer cardiovascular disease. This study aimed to investigate effect on AGE-induced myocardial injury vitro.Cell viability test was used select 40 µM concentrations treat H2c9 cells. The cell apoptosis detected by flow...

Abstract Background Diabetic nephropathy (DN) is one of the most severe complications diabetes mellitus, which characterized by early occurrence albuminuria and end-stage glomerulosclerosis. Senescence autophagy podocytes play an important role in DN development. Human umbilical cord-derived mesenchymal stem cells (hucMSCs) have potential treatment its complications. However, hucMSCs underlying mechanism remain unclear. Methods In vivo, a streptozotocin-induced diabetic male Sprague Dawley...

The present study aimed to investigate the protective effects of ghrelin against oxidative stress, inducible nitric oxide synthase (iNOS) and inflammation in a mouse model myocardial ischemia/reperfusion injury (MIRI). In addition, determine its underlying mechanisms. A MIRI was used vivo, order ascertain on MIRI. Commercial kits were measure serum levels creatine kinase (CK) lactate dehydrogenase (LDH) mice. Furthermore, Evan's Blue-triphenyltetrazolium chloride solution analyze infarct...

Tetrahydropalmatine (THP) regulates mitochondrial function in vascular smooth muscle cells (VSMCs) to prevent or alleviate atherosclerosis (AS), with unclear specific mechanism. AS models were constructed by oxidized low-density lipoprotein (ox-LDL)-treated VSMCs. Cell counting kit-8 for cell viability, wound scratch assay migration, and flow cytometry cycle, intracellular reactive oxygen species, membrane potential (MMP) performed. Malondialdehyde (MDA) superoxide dismutase (SOD) levels...

Diabetic foot ulcer (DFU) is a main diabetic complication with unmet treatment needs. This study applied human umbilical cord-derived mesenchymal stem cells-hyaluronic acid (hucMSCs-HA) gel to treat DFU in noninvasive external way and investigated its paracrine action mechanism. In this study, after analyzing the physical biological properties of HA gel, hucMSCs-HA was 2 vivo models (types I II DFU), molecular mechanism investigated. To evaluate hucMSCs, hucMSCs-conditional medium (MSC-CM)...

Diabetic nephropathy (DN) is a common chronic complication of diabetes, for which acute glucose fluctuation (AGF) potential risk factor. Fluctuating hyperglycemia has been confirmed to induce more serious kidney damage than in diabetic rats; however, the mechanism remains unknown. The purpose this study was explore role AGF progression DN. Viability rat podocytes following 72‑h treatment detected using Cell Counting‑Kit‑8. rates apoptosis and level reactive oxygen species (ROS) were assessed...

The naringin extraction process was optimised using response surface methodology (RSM). A central component design adopted, which included four parameters: temperature (X1), material–liquid ratio (X2), time (X3), and ultrasonic frequency (X4) of 74.79 °C, 1.58 h, 1:56.51 g/mL, 28.05 KHz, respectively. Based on these optimal conditions, tested to verify the model’s accuracy. Naringin yield 36.2502 mg/g, equivalent predicted 36.0124 mg/g. DM101 macroporous adsorption resin used purify...

C-peptide (CP) has demonstrated unique beneficial effects in diabetic nephropathy (DN), but whether and how CP regulates NF-κB its coactivator, p300, to suppress inducible iNOS antagonize DN are unknown. expression, nuclear translocation, colocalization binding of the inos promoter, bound NF-κB, histone 3 lysine 9 acetylation (H3K9ac) at sites were measured high glucose-stimulated mesangial cells. We evaluated pathologic changes, p300 contents rats. found that inhibited expression notably...

Acute lung injury (ALI) with uncontrolled inflammatory response has high morbidity and mortality rates in critically ill patients. Pathogen-associated molecular patterns (PAMPs) are involved the development of associated lethality. In this study, we investigated inhibit effect MS19, a microsatellite DNA-derived oligodeoxynucleotide (ODN) AAAG repeats, on induced by various PAMPs vitro vivo. parallel, DNA AAAC named as MS19-C, was used controls. We found that MS19 extensively inhibited...

Recent studies indicate a significant upregulation of gasdermin D (GSDMD) in acute kidney injury (AKI), severe medical condition characterized by high morbidity and mortality globally. In this study, we identified validated the therapeutic effects small molecule inhibitors targeting GSDMD pathway for AKI treatment. Using drug screening assay, evaluated thousands molecules from DrugBank against Lipopolysaccharide (LPS) Nigericin-stimulated immortalized bone marrow-derived macrophages (iBMDMs)...

Objective Oxidative stress and free radicals have been indicated to be associated with increased risk of various cancers. Paraoxonase 1 ( PON1) gene, encoding PON1 protein, plays an important role as endogenous free-radical scavenging molecule. The aim the present study was determine whether genetic polymorphisms gene are prognosis non–small cell lung cancer (NSCLC). Methods A total 356 patients NSCLC 184 healthy controls were enrolled in this study. Q192R L55M genotyped using polymerase...

Hyperlipidemia (HLP) is a lipid metabolism disorder that can induce series of cardiovascular and cerebrovascular diseases, such as atherosclerosis, myocardial infarction, coronary heart disease, stroke, which seriously threaten human health. Tetrahydropalmatine (THP) component the plant Rhizoma corydalis has been shown to exert hepatoprotective anti-inflammatory effects in HLP. However, whether THP regulates peroxidation hyperlipidemia, endoplasmic reticulum (ER) stress inflammasome...

AMPK is an important regulator of cellular glycolipid metabolism and in-volved in inflammation oxidative stress various diseases. closely related to a family nucleotide-binding oligomeric domain-like receptors, activation the signaling pathway prevents assembly NLRP3 inflammatory vesicles, vesicles are series protein complexes that mediate maturation release downstream factors interleukin-18 (IL-18) interleukin 1β (IL-1β), leading apoptosis causing responses. enhances utilization energy by...

Yiqi Huoxue recipe can delay the progression of diabetic nephropathy, but its treatment mechanism is still unclear. We aimed to explore effects on autophagy in nephropathy and underlying mechanism.All rats were randomly divided into seven groups. The body weight, kidney blood glucose, glycated hemoglobin, urine protein, microprotein, creatinine, urea nitrogen, triglyceride, lipoprotein analyzed. HE, Masson, periodic acid-Schiff staining used detect severity pathological changes kidneys....

ABSTRACTTo determine the clinical efficacy of Zuqing Xu 'Wuduling' powder for snake injury on swelling affected limb bitten by Agkistrodon halys. Sixty-five patients with halys bite were assigned to a treatment group (n = 35) or control 30). The was additionally given dressing locally based therapy conventional Western medicine. Clinical and improvement traditional Chinese medicine symptoms signs in two groups evaluated. showed notably higher cure rate than group. After 3 days therapy, pain...