Elucidation of the molecular targets and pathways regulated by tumour-suppressive miRNAs can shed light on oncogenic metastatic processes in prostate cancer (PCa). Using miRNA profiling analysis, we find that miR-188-5p was significantly down-regulated PCa. Down-regulation is an independent prognostic factor for poor overall biochemical recurrence-free survival. Restoration PCa cells (PC-3 LNCaP) suppresses proliferation, migration invasion vitro inhibits tumour growth metastasis vivo. We...

Zinc oxide nanoparticles (nZnO) have been widely used in the medicine field. Numerous mechanistic studies for nZnO's anticancer effects are merely performed under high concentration exposure. However, possible mechanisms of epigenetic dysregulation induced by low doses nZnO unclear.nZnO were characterized and bladder cancer T24 cells treated with 48 hrs at different exposure concentrations. Cell cycle, apoptosis, cell migration invasion determined. We qRT-PCR, Western blot chromatin...

Abstract With the rapid development of nanotechnology, nanomaterials are now being used for cancer treatment. Although studies on application silver nanoparticles in treatment burgeoning, few have investigated toxicology mechanisms autophagy cells under exposure to sublethal nanoparticles. Here, we clarified distinct regulation prostate PC‐3 exposure. Silver nanoparticle caused lysosome injury, including decline lysosomal membrane integrity, decrease quantity, and attenuation protease...

Abstract The pathogenesis of renal calcium-oxalate (CaOx) stones is complex and influenced by various metabolic factors. In parallel, palmitic acid (PA) has been identified as an upregulated lipid metabolite in the urine serum patients with CaOx via untargeted metabolomics. Thus, this study aimed to mechanistically assess whether PA involved stone formation. Lipidomics analysis PA-treated tubular epithelial cells compared control samples revealed that α-linoleic α-linolenic were desaturated...

The phospholipid-modifying enzyme MBOAT2 plays a crucial role in iron homeostasis by inhibiting sequestration, thus preventing iron-induced cell death. It achieves this remodeling the phospholipid composition of membranes through metabolism. Although multiple studies have highlighted significance tumorigenesis, comprehensive pan-cancer analysis has not been conducted to date. In study, we analyzed expression levels using RNA sequencing data from TCGA and GTEx databases. We also investigated...

Background Lysosome-associated protein transmembrane 4b-35 (LAPTM4B-35) is a member of the mammalian 4-tetratransmembrane spanning superfamily, which overexpressed in several solid malignancies. However, expression LAPTM4B-35 and its role progression prostate cancer (PCa) unknown. The aim present study was to investigate PCa potential relevance clinicopathological variables prognosis. Methods Immunohistochemistry used determine 180 tissues comparison with normal benign prostatic hyperplasia...

Protein tyrosine kinase 7 (PTK7) has been studied in various tumors, but its role prostate cancer remains unknown. This study is aimed to investigate the prognostic and predictive significance of PTK7 patients with cancer. expression was evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) Western blot analysis 20 pairs benign prostatic hyperplasia specimens specimens. Then, we examined immunohistochemical 180 clinical significances. Elevated significantly...

Oxidative stress is a causal factor and key promoter of urolithiasis associated with renal tubular epithelium cell injury. The present study was designed to investigate the preventive effects metformin on injury induced by oxalate stone formation in hyperoxaluric rat model. MTT assays were carried out determine protection from oxalate‐induced cytotoxicity. intracellular superoxide dismutase (SOD) activities malondialdehyde (MDA) levels measured vitro. Male Sprague‐Dawley rats divided into...

Objective: To compare the efficacy and safety of a single-use digital flexible ureteroscope (f-URS) reusable f-URS (URF-V) for treatment renal stones in adults. Patients Methods: In this randomized open-label noninferiority trial, we randomly selected patients with to receive ureteroscopy through (ZebraScope™; Happiness Workshop, Beijing, China) or URF-V (Olympus, Tokyo, Japan). The primary endpoint was 1-month postsurgical stone-free rate (SFR). secondary endpoints assessed were...

Calcium oxalate kidney stone has become an urgent issue due to its high incidence and recurrence rate. Thus, it is necessary explore for mechanisms of calcium stones formation. Previous studies demonstrated that crystals could induce the activation nucleotide‑binding domain leucine‑rich repeat‑containing family pyrin domain‑containing 3 (NLRP3) inflammasome change renal tubular epithelium adhesion. However, type molecular mechanism NLRP3 inflammasome‑mediated formation still need be further...

The objective of the study is to clarify mechanism p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway in change crystal adhesion rat renal tubular epithelial cells (NRK-52E) induced by calcium oxalate monohydrate (COM) crystals. NRK-52E were divided into COM crystal-treated group and control according whether cell culture medium contains different concentrations lactate dehydrogenase both determined after being cultured for 24 h. Protein RNA extracted from groups at time...

Abstract The aim is to compare the efficacy and safety between single percutaneous nephrolithotomy (sPNL) antegrade flexible ureteroscopy-assisted (aPNL) for treatment of staghorn calculi. A prospective randomized controlled study was conducted at Second Hospital Tianjin Medical University. total 160 eligible patients were included, with 81 in sPNL group 79 aPNL group. first compared overall differences aPNL. Then, divided into two subgroups: Group 1 (with less than 5 stone branches) 2 or...

Background Nucleostemin is essential for the proliferation and survival of stem cancer cells, but it unknown whether this newly identified molecule involved in prostate pathogenesis. Methods Total RNA protein were extracted from tissues PC-3, LNCap DU145 cell lines. The nucleostemin mRNA expression measured by RT-PCR Western blot. Immunohistochemistry was also used to detect PC-3 cells. A specific, short hairpin RNA, plasmid transfect changes gene detected proliferative capacity cells...

Although we and other studies indicated ZNF217 expression was increased in prostate cancer (PCa), the factors mediating its misregulated their oncogenic activity remain largely unexplored. Recent evidence demonstrated that ferroportin (FPN) reduction lead to decreased iron export intercellular consequently aggravates effects of iron. In present study, identified as a transcriptional repressor inhibits FPN expression. Increased led concentration, coupled with resultant intracellular...

While recent research has shown that expression of RABEX-5 in breast cancer and colorectal a crucial impact on tumor development, there is little information regarding prostate cancer. This study investigated the by real time quantitative polymerase chain reaction evaluated its association with clinicopathological variables, including patient prognosis. A total 180 patients primary treated radical prostatectomy were enrolled. Real was utilized to investigate mRNA level paired cancer/adjacent...

The incidence and prevalence of urinary stone are increasing throughout the world. Compared to past, recent demographics patient with urolithiasis compositions strikingly different. Furthermore, clinical studies implied that seasonal cyclicity might influence distribution composition.We sought determine trends in pathogenesis based on analyses. Between 2002 2014, a total 2,383 eligible samples from different patients were collected our center. Infrared spectroscopy was used for calculi...

Bilateral upper urinary tract stones are significantly related to renal function damage. However, few studies characterized the risk factors of bilateral stones. We retrospectively enrolled 3905 patients with from March 2019 2022 at Second Hospital Tianjin Medical University. Patients were divided into two groups according location stones, and data evaluated. In this study, 2485 unilateral 1420 stone included. Multivariate logistic regression analysis showed that BMI, gout,...

To evaluate the clinical efficacy, safety, and costs of percutaneous occlusive balloon catheter-assisted ureteroscopic lithotripsy (POBC-URSL) for large impacted proximal ureteral calculi.156 patients with stones ≥1.5 cm in size were randomized to (URSL), POBC-URSL, nephrolithotomy (PNL) group between May 2010 2013. For URSL, calculi disintegrated assistance anti-retropulsion devices. POBC-URSL was performed an 8F catheter. PNL finished combination ultrasonic a pneumatic lithotripter. A...

Treatment targeting osteopontin (OPN) and monocyte chemoattractant protein 1 (MCP‑1) has been recognized as a novel approach in renal crystal formation. The present study was designed to investigate the suppressive effects of metformin on nephrolithiasis formation its potential mechanism. cytotoxicity MDCK HK‑2 cells determined using Cell Counting Kit‑8 assay vitro. Subsequently, mRNA transcription expression levels MCP‑1 OPN were detected by reverse transcription‑quantitative‑polymerase...

Objective: To compare the efficacy and safety of a single-use digital flexible ureteroscope (FURS) reusable FURS for treatment lower pole stones (LPS) smaller than 20 mm. Patients Methods: We analyzed data 49 patients with LPS from our previous multicenter, randomized, open-label clinical trial in four hospitals China. All underwent ZebraScope™ (trial group) or URF-V (control group). The endpoints assessed were 1-month postsurgical stone-free rate (SFR), operative time, length postoperative...