A5101817802- Prenatal Screening and Diagnostics
- Natural product bioactivities and synthesis
- Phytochemical Studies and Bioactivities
- Genomic variations and chromosomal abnormalities
- Marine Sponges and Natural Products
- Parvovirus B19 Infection Studies
- Cell death mechanisms and regulation
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Hemoglobinopathies and Related Disorders
- Cervical Cancer and HPV Research
- Remote Sensing and Land Use
- Genetic and Kidney Cyst Diseases
- Iron Metabolism and Disorders
- Regional Economic and Spatial Analysis
- RNA modifications and cancer
- Traditional Chinese Medicine Analysis
- Immunodeficiency and Autoimmune Disorders
- Mesenchymal stem cell research
- Renal and related cancers
- Phytochemistry and Bioactive Compounds
- Medicinal Plant Pharmacodynamics Research
- Genetic Syndromes and Imprinting
- Radiopharmaceutical Chemistry and Applications
West China Second University Hospital of Sichuan University
2014-2025
Sichuan University
2014-2025
Zigong First People's Hospital
2024
Weatherford College
2024
Sichuan Agricultural University
2020
Ningbo No.6 Hospital
2019
Hebei North University
2014
Capital Medical University
2013
Ministry of Education of the People's Republic of China
2013
Beijing Anzhen Hospital
2013
Ubiquitination is a reversible process of posttranslational protein modification through the action family deubiquitylating enzymes which contain ubiquitin-specific protease 9x (USP9X). Recent evidence indicates that USP9X involved in progression various human cancers. The aim was to detect expression from normal epithelium invasive esophageal squamous cell cancer (ESCC) and evaluate relevance tumor prognosis.In this study, immunohistochemical analysis performed on tissues constructed ESCC...
Abstract Thalassemia is a group of common hereditary anemias that cause significant morbidity and mortality worldwide. However, precisely diagnosing thalassemia, especially rare thalassemia variants, still challenging. Long-range PCR long-molecule sequencing on the PacBio Sequel II platform utilized in this study could cover entire HBA1 , HBA2 HBB genes, enabling diagnosis most types variants. In study, 100 cases suspected were subjected to traditional testing third-generation for genetic...
Abstract Rhizoma Paridis, a traditional Chinese medicine, has shown promise in cancer prevention and therapy. In the present study, we isolated Paris Saponin I (PSI), an active component of paridis, evaluated its effects on panel human cell lines mouse model ovarian to explore mechanisms activity. PSI had more potent selective cytotoxic tumor than etoposide had, promoting dramatic G2-M phase arrest apoptosis SKOV3 cells time- dose-dependent manner. Furthermore, treatment increased levels...
Abstract Background Couples with balanced chromosome rearrangement (BCR) are at high risk of recurrent miscarriages or birth defects due to chromosomally abnormal embryos. This study aimed provide real-world evidence the euploidy rate blastocysts from couples BCR using preimplantation genetic testing (PGT) and guide pretesting counselling. Methods A continuous four-year PGT data were retrospectively analyzed. Biopsied trophectoderm cells amplified whole genome amplification, next-generation...
As the development of molecular diagnostic methods, a large number clinically relevant or disease-related copy variations (CNVs) could be detected, and demand for genetic counselling clinical treatment is also increasing. For patients with pathogenic likely CNVs, preimplantation testing (PGT) provide feasible path to prevent inheritance disorder in offspring. In this study, we included couple 1q21.1 recurrent microduplication conduct diagnosis PGT application. The optical genome mapping...
In cervical cancer, one of the most common malignant tumors in women worldwide, miR-126 has been reported to exhibit decreased expression. However, its role cancer cell proliferation and drug sensitivity remained relatively unexplored. Here, we compared expression tissues (n = 20) with that normal tissue using quantitative RT-PCR. The viability Siha cells was further measured by MTT assay after transfection mimic (Siha-miR-126 mimic) or microRNA negative control (Siha-miR NC) treatment...
Human papillomavirus (HPV) infection plays an etiological role in the development of cervical dysplasia and cancer. Amplification human telomerase gene (hTERC) over expression were found to be associated with tumorigenesis. This study was performed analyze genomic amplification hTERC gene, activity association HPV different stages intraepithelial neoplasia (CIN) We studying progression uterine invasive cancer, proposed adjunct method for cancer screening. Exfoliated cells collected from 114...
Abstract Background As an acute-phase protein, serum amyloid A (SAA) is expressed primarily in the liver. However, its expression extrahepatic tissues, especially tumor was also demonstrated recently. In our study, we investigated of SAA uterine cervical carcinomas, and results suggested potential as a biomarker. Methods Quantitative real-time polymerase chain reaction (RT-PCR), immunohistochemistry (IHC) enzyme-linked immunosorbent assay (ELISA) were used to evaluate gene protein levels...
Abstract A prospective analysis investigating the associations between pathogenic copy number variations (pCNVs) and ultrasound soft markers (USMs) in fetuses evaluating clinical value of variation sequencing (CNV-seq) such pregnancy studies was carried out. 3,398 unrelated Chinese women with singleton pregnancies undergone amniocentesis at 18–36 weeks gestation for fetal CNV-seq were included. According to prenatal screening results, samples divided into 3 groups: normal (n = 2616),...
Abstract The autosomal dominant form of polycystic kidney disease (ADPKD) is the most common hereditary that causes late-onset renal cyst development and end-stage disease. Preimplantation genetic testing for monogenic (PGT-M) has emerged as an effective strategy to prevent pathogenic mutation transmission rely on SNP linkage analysis between pedigree members. Yet, it remains challenging establish reliable PGT-M methods ADPKD cases or other diseases with de novo mutations without a family...
Persistent high-risk human papillomavirus (HR-HPV) infection has been implicated in the development of high-grade cervical intraepithelial neoplasia (CIN) and cancer. Invariant natural killer T (iNKT) cells produce large amounts cytokines to regulate immune responses. However, role iNKT persistent HPV-infected tissues is unknown. In our study, 201 patients with diagnoses ranging from normal ectocervical tissue CINIII June 2010 May 2012 were enrolled. HPV DNA types detected using hybrid...
Introduction: X-linked recessive chronic granulomatous disease (XR-CGD) is a severe primary immunodeficiency principally caused by CYBB (OMIM: 300481) gene variant. Recurrent fatal bacterial or fungal infections are the main clinical manifestations of XR-CGD. Patient concerns: In current case, in vitro fertilization (IVF) associated with preimplantation genetic testing for monogenic disorder (PGT-M) was applied Chinese couple who had given birth to boy Diagnosis: Next-generation...
Abstract To discuss combinations of traditional screening and noninvasive prenatal (NIPS) to compare which is the most suitable first-line approach NIPS, pregnant women were recruited in this retrospective observational study. Pregnant underwent one four tests. The 9 contingent models combined by high risk cut-offs 1:50, 1:100, 1:270 intermediate 1:1000, 1:1500, 1:2000. We analyzed cost performance various with different cut-offs. Compared other tests, first-trimester (CFTS) had lowest...
To assess the value of copy number variation analysis based on next generation sequencing (CNV-seq) in prenatal diagnosis for women at advanced maternal age.A prospective was carried out who underwent amniocentesis 18~36 weeks gestation fetal CNV-seq age.For 1461 unrelated Chinese with a singleton pregnancy, performed all samples successfully. The proportion chromosomal abnormalities 2.3% (34/1461), which 44.12% were submicroscopic variations (<5 Mb).Pregnant an age should be informed not...
Abstract Background Intraperitoneal (i.p.) models that accurately mimic the feature behavior of human ovarian cancer are required to investigate pathology and therapeutics disease. However, established i.p. which well-characterized reliable few. The purposes this study establish a representative mice model disease analyze consequent pathology. Methods Fresh tumor cells fiom ascites patient were injected into female NOD/SCID intraperitoneally. Histology, Cytogenetic,...
1‑calcium phosphate‑uracil (1‑CP‑U), a synthetic pyrimidine derivative, has been documented to demonstrate variety of different biological activities. However, the potency and mechanisms this agent's anti-cancer activity have not elucidated date. In present study, anti‑cancer effects 1‑CP‑U were examined in range vitro assays. Different cell lines treated with at varied concentrations (0.7, 1.0, 1.4 µmol/l) for indicated durations. The proliferation was then by MTT assay. cellular apoptotic...
Objective: The purpose of this research was to attempt a preliminary study noninvasive prenatal diagnosis Down syndrome in Southwest Chinese gravidas by using the plasma placental RNA allelic ratio. Methods: genotypes single-nucleotide polymorphisms (SNPs) located transcribed regions gene PLAC4 were detected population samples collected China polymerase chain reaction–restriction fragment length polymorphism, and SNPs with higher heterozygosity selected. Mass spectrometer analysis adopted,...
Chromosome rearrangement is one of the main causes abortion. In individuals with double chromosomal rearrangements, abortion rate and risk producing abnormal embryos are increased. our study, preimplantation genetic testing for structural (PGT-SR) was performed a couple because recurrent karyotype male 45, XY der (14; 15)(q10; q10). The PGT-SR result embryo in this vitro fertilization (IVF) cycle showed microduplication microdeletion at terminals chromosomes 3 11, respectively. Therefore, we...