A5075866053- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Cancer Risks and Factors
- Immunotherapy and Immune Responses
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Cancer survivorship and care
- Advanced biosensing and bioanalysis techniques
- Phagocytosis and Immune Regulation
- Ovarian function and disorders
- Protein Degradation and Inhibitors
- Ferroptosis and cancer prognosis
- Cellular transport and secretion
- Reproductive Health and Contraception
- Pregnancy and Medication Impact
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Family Support in Illness
- Thermal properties of materials
- Assisted Reproductive Technology and Twin Pregnancy
Institut de Recherche en Cancérologie de Montpellier
2019-2025
Inserm
2008-2025
Université de Montpellier
2022-2025
Institut Regional du Cancer de Montpellier
2021-2024
Institut Curie
2008-2013
Université Paris Cité
2012-2013
Délégation Paris 5
2013
Universidade Nova de Lisboa
2012
Centre National de la Recherche Scientifique
2012
University of Lisbon
2012
During progression from single cancer cells to a tumor mass and metastases, send signals that can subvert their tissue microenvironment. These involve soluble molecules various extracellular vesicles, including particular type termed exosomes. The specific roles of exosomes secreted in the microenvironment, however, is unclear. small GTPases RAB27A RAB27B regulate exocytosis multivesicular endosomes, which lead exosome secretion, human HeLa cells. Here, we used mouse models show Rab27a...
Exosomes are extracellular vesicles of 50 to 100 nm in diameter, released by many cell types. formed inside the intracellular endosomal compartments and secreted upon fusion these with plasma membrane. Cells also secrete other types membrane vesicles, for instance, outward budding from membrane, although some them clearly differ exosomes their structural features (larger size), others possibly more difficult separate. Here, using Rab27a inhibition modulate exosome secretion, we show...
Abstract Expression of non-self antigens by tumors can induce activation T cells in vivo, although this lead to either immunity or tolerance. CD8+ T-cell be direct (if the tumor expresses MHC class I molecules) indirect (after capture and cross-presentation dendritic cells). The modes antigen vivo remain unclear. Here we examine immunogenicity same model secreted live association with membrane vesicles (exosomes) as a soluble protein. We have artificially addressed coupling it factor...
Abstract Background The cutoff of <1% positive cells to define estrogen receptor (ER) negativity by immunohistochemistry (IHC) in breast cancer (BC) is debated. We explored the tumor immune microenvironment and gene-expression profile patients with early-stage HER2-negative ER-low (ER 1%-9%) BC, comparing them ER-negative <1%) ER-intermediate 10%-50%) tumors. Methods Among 921 I-III, ER ≤50%, BCs, tumors were classified as (n = 712), 128), or 81). Tumor-infiltrating lymphocytes...
Abstract Importance Data on fertility after breast cancer (BC) relative to the general population are lacking. Objective To compare time-to-pregnancy between women with and without prior BC seeking become pregnant. Design Prospective exposed-unexposed cohort study. Women were included from March 13, 2018 June 27, 2019. collected every six months via online questionnaires for up three years. Setting Participants recruited through French collaborative network research Seintinelles*. Exposed...
To understand the physiological functions of exosomes, we have recently used inhibition Rab27a, which prevents exosome release but also alters other secretion pathways. Our work demonstrates that exosomes by some tumors in vivo can influence immune microenvironment to promote tumor progression, this phenomenon cannot be generalized all and exosomes.
Background: Triple-negative breast cancers (TNBCs) have a worse prognosis, but might respond to immunotherapies. Macrophages are plastic cells that can adopt various phenotypes and functions. Although they major immune population in TNBCs, the relationship between tumor-associated macrophages (TAMs) TNBC progression has been rarely explored, with controversial results. Methods: We evaluated prognostic impact of TAMs, quantified by immunohistochemistry anti-CD68, -IRF8, -CD163, -CD206...
Background T cell immunoreceptor with Ig and ITIM domains (TIGIT) interacts poliovirus receptor (PVR) to contribute cancer immune escape. Recently, TIGIT PVR have been identified as promising immunotherapy targets. Their gene expression is upregulated in many solid tumors, but their protein level not well documented, particularly triple negative breast (TNBC), the subtype that most benefit from immunotherapy. Methods levels were assessed by immunohistochemistry 243 surgically resected...
For several years, the AXL tyrosine kinase receptor, a member of Tyro3-Axl-Mer (TAM) family, has been considered new strategic target in oncology. overexpression is common solid tumors and associated with poor prognosis. In this context, detection subset circulating tumor cells (CTCs) that express (AXL
The recommended cut-off of <1% positive cells to define estrogen receptor (ER) negative status in breast cancer (BC) is highly debated. We compared the tumor immune microenvironment (TME) HER2-neg BC according ER (ER-neg: 0% vs ER-low: 1-9%). This multicentric study included patients with stage I-III from centers across Italy and France: Istituto Oncologico Veneto IOV; Montpellier Cancer Institute MCI; Nazionale Tumori Milano; Europeo di Oncologia. Tumor samples were reviewed for ER: ER-neg...
Sexual quality of life (QoL) is affected during and after breast cancer (BC) treatment. The purpose was to investigate sexual global QoL patient-reported measures address this issue in young women (< 51 years) with BC the acute treatment phase, adjuvant endocrine therapy.
To apply text mining (TM) technology on electronic medical records (EMRs) of patients with breast cancer (BC) to retrieve the occurrence a pregnancy after BC diagnosis and compare its performance manual curation.The training cohort (Cohort A) comprised 344 age ≤ 40 years old treated at Institut Curie between 2005 2007. Manual curation consisted in manually reviewing each EMR pregnancies. TM first applying keyword filter ("accouch*" or "enceinte," French terms for "deliver*" "pregnant,"...
In triple-negative breast cancer (TNBC), the most immunogenic type, tumor-infiltrating lymphocytes (TILs) are an independent prognostic factor. Tertiary lymphoid structures (TLS) important TILs source, but they not integrated in current criteria.
To compare the prevalence of contraception in breast cancer (BC) patients at risk unintentional pregnancy (i.e. not currently pregnant or trying to get pregnant) and matched controls.The FEERIC study (Fertility, Pregnancy, Contraception after BC France) is a prospective, multicenter case-control study, including localized aged 18-43 years, for age parity cancer-free volunteer controls 1:2 ratio. Data were collected through online questionnaires completed on Seintinelles research platform.In...
Abstract Objective To study fertility concerns and oncofertility practices at time of breast cancer (BC) diagnosis. Design The FEERIC (Fertility, Pregnancy, Contraception after BC in France) is a prospective, multicenter study. Setting Web-based collaborative research platform Seintinelles . Patients 517 patients with prior diagnosis free from relapse aged 18 to 43 years inclusion (from 12 th March 2018 27 June 2019). Intervention Baseline online self-administered questionnaires. Main...
<div>Abstract<p>Expression of non-self antigens by tumors can induce activation T cells <i>in vivo</i>, although this lead to either immunity or tolerance. CD8<sup>+</sup> T-cell be direct (if the tumor expresses MHC class I molecules) indirect (after capture and cross-presentation dendritic cells). The modes antigen vivo</i> remain unclear. Here we examine immunogenicity same model secreted live in association with membrane vesicles (exosomes) as a...
<div>Abstract<p>During progression from single cancer cells to a tumor mass and metastases, send signals that can subvert their tissue microenvironment. These involve soluble molecules various extracellular vesicles, including particular type termed exosomes. The specific roles of exosomes secreted in the microenvironment, however, is unclear. small GTPases RAB27A RAB27B regulate exocytosis multivesicular endosomes, which lead exosome secretion, human HeLa cells. Here, we used...
<p>PDF file - 77K, Metastasis formation after i.v. injection and in vitro growth of 4T1; by 4T1 is decreased (A), whereas tumors not affected (B) Rab27a inhibition</p>
<p>PDF file - 158K, Involvement of the immune system in differential growth TS/A, 4T1, and their sh27a-expressing counterparts; A-Examples FACS stainings cytospin analysis CD11b, CD11c, Ly6C Ly6G spleens tumor-bearing mice. B, C- Growth Scr- sh27a-4T1 Rag2-/- or CD4+ T cell-depleted hosts</p>
<p>PDF file - 113K</p>