A5079990535- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Bacterial Genetics and Biotechnology
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Ion channel regulation and function
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- Bioinformatics and Genomic Networks
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Viral gastroenteritis research and epidemiology
- Heat Transfer and Optimization
- Cellular transport and secretion
- Antibiotic Resistance in Bacteria
- Computational Drug Discovery Methods
- Microtubule and mitosis dynamics
- Energy and Environmental Systems
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
- SARS-CoV-2 and COVID-19 Research
Yonsei University College of Dentistry
2021-2022
Yonsei University
2021-2022
Agency for Defense Development
2020
Gwangju Institute of Science and Technology
2003-2013
Korea Research Institute of Bioscience and Biotechnology
2005-2006
In Gram-negative bacteria, proper placement of the FtsZ ring, mediated by nucleoid occlusion and activities dynamic oscillating Min proteins MinC, MinD MinE, is required for correct positioning cell division septum. MinE a topological specificity factor that counters activity MinCD inhibitor at mid-cell site. Its structure consists an anti-MinCD domain topology (TSD). Previous NMR analysis truncated Escherichia coli showed TSD contains long alpha-helix two anti-parallel beta-strands, which...
D-alanine:D-alanine ligase (Ddl) catalyses the dimerization of D-alanine before its incorporation in peptidoglycan precursors. The synthesis begins with an attack on first by γ-phosphate adenosine triphosphate (ATP) to yield acylphosphate. That is followed amino group second D-alanine, which eliminates phosphate and produces dipeptide.1, 2 Peptidoglycan biosynthesis has long been attractive target for antibacterial drugs, such as D-cycloserine, glycopeptide antibiotics (vancomycin...
The epithelial-mesenchymal transition (EMT) comprises an important biological mechanism not only for cancer progression but also in the therapeutic resistance of cells. While importance protein abundance EMT-inducers, such as Snail (SNAI1) and Zeb1 (ZEB1), during EMT is clear, reciprocal interactions between untranslated regions (UTRs) EMT-inducers via a competing endogenous RNA (ceRNA) network have received little attention. In this study, we found synchronized transcript mediated by...
Jun Seop Yun, Hyeeun Song, Nam Hee Kim, So Young Cha, Kyu Ho Hwang, Jae Eun Lee, Cheol-Hee Jeong, Sang Hyun Seonghun Eunae Sandra Cho, Sil and Jong In Yook. Mol. Cells 2022;45:911-22. https://doi.org/10.14348/molcells.2022.0130
African swine fever virus (ASFV) causes a highly contagious and severe hemorrhagic viral disease with high mortality in domestic pigs of all ages. Although the is harmless to humans, ongoing ASFV epidemic could have economic consequences for global food security. Recent studies found few antiviral agents that can inhibit infections. However, currently, there are no vaccines or drugs. Hence, an urgent need identify new drugs treat ASFV. Based on structural information data targets ASFV, we...
Although protein-protein interactions (PPIs) have emerged as the basis of potential new therapeutic approaches, targeting intracellular PPIs with small molecule inhibitors is conventionally considered highly challenging. Driven by increasing research efforts, success rates increased significantly in recent years. In this study, we analyze physicochemical properties 9351 non-redundant present iPPI-DB and TIMBAL databases to define a computational model for active compounds acting against PPI...
Peptidoglycan biosynthesis is initiated with the formation of UDP-N-acetylmuramic acid via stepwise action two enzymes, UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) and UDP-N-acetylenolpyruvylglucosamine reductase (MurB), which has proven to be attractive targets for development antimicrobial agents. MurA catalyzes first stage reaction, transferal enolpyruvate moiety phosphoenolpyruvate 3′-hydroxyl release inorganic phosphate.1 The resulting intermediate,...
We have determined the crystal structure of porcine quinolinate phosphoribosyltransferase (QAPRTase) in complex with nicotinate mononucleotide (NAMN), which is first a mammalian QAPRTase its reaction product. The was from protein obtained kidney. Because full sequence not available either or nucleotide databases, cDNA synthesized using reverse transcriptase-polymerase chain to determine amino acid sequence. revealed that QAPRTases hexameric similar other eukaryotic QAPRTases, such as human...
Ryanodine receptor 1 (RyR1) is a large homotetrameric calcium channel that plays pivotal role in skeletal muscle contraction. Sequence comparison and mutagenesis studies indicate the pore architecture of RyR1, including last two transmembrane helices luminal loop linking them, similar to bacterial KcsA K(+) channel. Here, we describe overexpression purification C-terminal polyhistidine-tagged RyR1 pore-forming region. The nonionic detergent lauryldimethylamine oxide (LDAO) was selected for...
Orotic acid phosphoribosyltransferase (PyrE) (EC 2.4.2.10) is a key enzyme in de novo uridine monophosphate (UMP) biosynthesis. It catalyzes the reaction between orotic and 5-phosphoribosyl-1-pyro-phosphate (PRPP) to yield orotidine (OMP), which transformed by decarboxylation. H. pylori PyrE was crystallized at 294 ± 1 K hanging drop vapor-diffusion method. The crystals belong space group P212121 with unit-cell dimensions = 95.8, b 104.9, c 281.1 Å, β γ 90°. A set of diffraction data...
ABSTRACT A structural protein of SARS-CoV-2, nucleocapsid (N) is abundantly expressed during viral replication. The N phosphorylated by glycogen synthase kinase (GSK)-3 on the serine/arginine (SR) rich motif located in disordered regions. Although phosphorylation GSK-3β constitutes a critical event for replication, molecular mechanism underlying not well understood. In this study, we found putative alpha-helix L/FxxxL/AxxRL known as GSK-3 interacting domain (GID), commonly many endogenous...
In this paper, I introduce Phase-Change Cooling for thermal management of high power devices that can be applied to High Power Laser and Electric Propulsion Systems which are composed multiple distributed superheat sources. good used efficient cooling their heat has extremely efficiency two-phase transport by utilizing vaporization, relatively low flow rates reduced pumps power. And suggest TPI(Thermal Performance Index) is a quantitative performance index management. quantify the...