Circulating tumour cells (CTCs) are shed from a primary and circulate in the peripheral blood after passing through drainage vein. In previous studies, we showed that high numbers of CTCs were detected pulmonary venous most patients with resectable lung cancer, whereas only low some patients. Accordingly, this prospective study was conducted to assess changes vein (PV) during cancer surgery. A total 30 consecutive peripheral-type who underwent lobectomy (or right upper middle bilobectomy)...

Malignant pleural mesothelioma (MPM) is an aggressive malignant tumor of mesothelial origin that shows a limited response to conventional chemotherapy and radiotherapy. Therefore, diagnosing MPM early very important. Some researchers have previously reported high-mobility group box 1 (HMGB1) was correlated with pulmonary fibrosis. involves the transformation cells, which originate from mesenchymal cells similar lung fibroblasts. Here, we investigated serum levels HMGB1 in patients compared...

Abstract Pulmonary fibrosis in humans can occur as a result of large number conditions. In idiopathic pulmonary (IPF), function becomes progressively compromised resulting high mortality rate. Currently there are no proven effective treatments for IPF. We have recently reported that IL-6 and TGF-β1 plays an important role proliferation differentiation lung fibroblasts, all-trans-retinoic acid (ATRA) prevented bleomycin-induced through the inhibition these cytokines. Thalidomide (Thal) has...

Rationale: Although radiotherapy is effective in treating lung cancers, resultant pulmonary injury the main obstacle. Pulmonary fibrosis characterized by progressive worsening function leading to high incidence of death. Currently, however, there has been little progress preventive and therapeutic strategies.Objectives: Previously, we reported that all-trans-retinoic acid (ATRA) reduced both irradiation-induced interleukin (IL)-6 production fibroblasts IL-6–dependent cell growth, also...

Malignant mesothelioma is an aggressive tumor of serosal surfaces, which refractory to current treatment options. Arsenic trioxide (As2O3) used clinically treat acute promyelocytic leukemia, and also inhibit proliferation several solid tumors including hepatoma, esophageal, gastric cancer in vitro. Here we found that As2O3 inhibited cell viability a line, NCI-H2052. induced apoptosis NCI-H2052 cells, was accompanied by activation c-Jun NH2-terminal kinase (JNK)1/2, extracellular...

Our earlier studies suggested crosstalk between IRS/PI3 kinase/PDK1/Akt/Rac1/ROCK and (Shc2/Grb2/SOS)/Ras/Raf/MEK/ERK pathways downstream PDGF-ββ receptor responsible for chemotaxis proliferation of malignant mesothelioma cells. The present study was conducted to obtain evidence this.To assess activation Akt, MEK, ERK, Western blotting carried out on MSTO-211H cells using antibodies against phospho-Thr308-Akt, phopho-Ser473-Akt, phospho-MEK, phopho-ERK1/2, ERK1/2. To knock-down PI3 kinase,...

Liver fibrosis with any aetiology, induced by the transdifferentiation and proliferation of hepatic stellate cells (HSCs) to produce collagen, is characterized progressive worsening in liver function, leading a high incidence death. We have recently reported that all-trans-retinoic acid (ATRA) suppresses lung fibroblasts prevents radiation- or bleomycin-induced fibrosis.We examined impact ATRA on carbon tetrachloride (CCl(4))-induced fibrosis. performed histological examinations quantitative...

Although high-dose thoracic radiotherapy is an effective strategy for some malignancies including lung cancers and malignant lymphomas, it often causes complications of radiation fibrosis. To study the mechanism initiating tissue fibrosis, we investigated irradiation-induced cytokine production from human fibroblastic cells found that IL-6 was stimulated by irradiation. autocrine growth factor myeloma cells, retinoic acid reported to inhibit their growth. Thus evaluated effect all- trans...

Summary Pulmonary fibrosis is characterized by progressive worsening of pulmonary function leading to a high incidence death. Currently, however, there has been little progress in therapeutic strategies for fibrosis. There have several reports on cytokines being associated with lung fibrosis, including interleukin (IL)-6 and transforming growth factor (TGF)-β1. We reported recently that two substances (ATRA thalidomide) preventive effects inhibiting IL-6-dependent proliferation...

Diffuse malignant peritoneal mesothelioma (DMPM) is an aggressive tumor of mesothelial origin that shows a limited response to cytoreductive surgery along with intraperitoneal chemotherapy. Therefore, early diagnosis DMPM very important. Some researchers have previously reported high-mobility group box 1 (HMGB1) was correlated pulmonary fibrosis. involves the transformation cells, which originate from mesenchymal similar lung fibroblasts. Here, we investigated serum levels HMGB1 in patients...

This study evaluates the risks of postoperative complications in 124 patients with non-small-cell lung cancer who received pre-operative induction chemoradiotherapy and surgery.All underwent surgery after therapy between January 1990 December 2003 were reviewed. We adopted univariate multiple logistic regression models to identify predictors that increased incidence complications.Of patients, 59 carboplatin docetaxel, 53 cisplatin etoposide, 12 other platinum-based combinations....

The mucin-type transmembrane glycoprotein podoplanin (also known as T1alpha, gp38 or Aggrus) is well established one of the lymphatic-specific markers. Podoplanin was also reported to be associated with tumor-induced platelet aggregation and tumor metastasis. Here, we generated a novel monoclonal antibody (clone; 7B10) that specifically recognized human podoplanin, assessed by enzyme-linked immunosorbent assay, Western blot analyses, immunohistochemistry flow cytometry. 7B10 stained not only...

Background: Malignant pleural mesothelioma (MPM) is an aggressive malignant tumor of mesothelial origin associated with asbestos exposure. MPM has a limited response to conventional chemotherapy and radiotherapy, so early diagnosis very important. This study investigated the effusion mesothelin levels in patients compared them those population non-malignant pleuritis or lung cancer involving effusion.

Accumulating evidence has suggested that free fatty acids (FFAs) interact with protein kinases and phosphatases. The present study examined the effect of FFAs on phosphatases Akt.Activities phosphatase 1 (PP1), 2A (PP2A), tyrosine 1B (PTP1B) were assayed under cell-free conditions. Phosphorylation Akt was monitored in MSTO-211H human malignant pleural mesothelioma cells without knocking-down phosphatidylinositol 3 kinase (PI3K) or 3-phosphoinositide-dependent kinase-1 (PDK1).In assay,...

A(3) adenosine receptor mediates apoptosis in cancer cells via diverse signaling pathways. The present study examined receptor-mediated Lu-65 cells, a human giant cell lung carcinoma line.MTT assay, TUNEL staining, real-time RT-PCR, Western blotting, and assay of caspase-3, -8, -9 activities were carried out or p53 was knocked-down by transfecting each siRNA into cells.Extracellular induces concentration (0.01-10 mM)-dependent manner, the effect inhibited inhibitor MRS1191 knocking-down p53....

Malignant pleural mesothelioma (MPM) is an aggressive malignant tumor of mesothelial origin associated with asbestos exposure. MPM has a limited response to conventional chemotherapy and radiotherapy, thus early diagnosis extremely critical. CT scans have accuracy in the differentiation between benign disease. Several studies reported that 18-fluorodeoxyglucose (FDG) positron emission tomography (PET) plays important role assessment thoracic malignancy such as lung cancer. Here, we...

Background/Aims: The present study investigated adenosine-induced apoptosis in human malignant pleural mesothelioma cells. Methods: MTT assay, TUNEL staining, flow cytometry using propidium iodide and annexin V-FITC, real-time RT-PCR, Western blotting, assay of caspase-3, -8, -9 activities were carried out cell lines such as NCI-H28, NCI-H2052, NCI-H2452, MSTO-211H cells, p53 or A3 adenosine receptor was knocked-down by transfecting each siRNA into Results: Adenosine induced all the cells...

Malignant mesothelioma is an aggressive tumor arising from mesothelial cells of serous membranes. Src family kinases (SFKs) have a pivotal role in cell adhesion, proliferation, survival and apoptosis. Here, we examined the effect SFK inhibitors NCI-H2052, ACC-MESO-4 NCI-H28 cells, lines Met5A, human non-malignant line. We found that PP2, selective inhibitor, inhibited activity induced apoptosis mediated by caspase-8 but not NCI-H2052 cells. Src, Yes, Fyn Lyn protein, which are members SFK,...

Sphingosine regulates cellular differentiation, cell growth, and apoptosis. The present study aimed at understanding sphingosine-regulated mesothelioma proliferation.Human malignant cells such as NCI-H28, NCI-H2052, NCI-H2452, MSTO-211H were cultured. siRNA to silence the protein kinase C (PKC)-δ-targeted gene was constructed transfected into cells. MTT assay, cycle analysis using a flow cytometry, cell-free PKC-δ assay carried out.For all types sphingosine inhibited growth in concentration...