Cultivation of oviduct epithelial cells on porous filters fosters in vivo-like morphology and functionality. However, due to the optical properties filter materials cells’ columnar shape, cell quality is hard assess via light microscopy. In this study, we aim evaluate transepithelial electrical resistance (TEER) measurement as a prognostic indicator for cultivation porcine (POEC). POEC were maintained four different types media 3 6 w achieve diverse culture qualities, TEER was measured...

Peritendinous tissue fibrosis which leads to poor tendon function is a worldwide clinical problem; however, its mechanism remains unclear. Transcription factor RelA/p65, an important subunit in the NF-κB complex, known have critical role many fibrotic diseases. Here, we show that RelA/p65 functions as core fibrogenic regulator adhesion and inhibition exerts anti-fibrogenic effect on peritendinous adhesion. We detected upregulation of pathway human using gene chip microarray assay revealed...

Throughout the estrous cycle oviduct epithelium undergoes dramatic morphological and functional changes. To elucidate cyclic cellular events associated regulation mechanisms of 17beta estradiol (E2) progesterone (P4), we mimicked stages in vitro using a culture system primary porcine cells (POEC). Cells were polarized an air/liquid interface then treated with E2 P4 for physiological time periods: In experiment 1, high concentration low 10 days resembled diestrus; 2, following previous...

Abstract We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional tissues, which generate fluid surrogates. These in vitro epithelia can be co-cultured with living zygotes and enable embryonic development up blastocyst stage without addition embryo medium. The described strategy is broadly applicable analyze early embryo-maternal interactions under standardized conditions.

<title>Abstract</title> Our aim was to apply Mendelian randomization find possible causal relationships between prostate cancer and central obesity. Based on waist-to-hip ratio GWAS data as the experimental group, we used 8 MR methods explore relationship cancer. Situation with P &lt; 0.05 suggested relationship. Gene annotation Ontology (GO) molecular functional enrichment analysis of meaningful SNPs were performed potentially significantly function enriched genes. Finally, searched for...

The aim of this study was to evaluate the effect ovarian stimulation with LH-containing gonadotropins (human menopausal gonadotropin, hMG), on ploidy human cleavage-stage-embryos.A total 104 women, at ages 27-43 years, undergoing one cycle controlled hyperstimulation for IVF in combination preimplantation genetic diagnosis, were eligible enrollment retrospective, cohort study. Ovarian included down-regulation long agonist and either recombinant FSH or hMG. Since embryos changes female age,...

Previous reports have demonstrated that embryonic stem cells were capable of differentiating into primordial germ through the formation embryoid bodies subsequently generated oocyte-like (OLCs). Such a process could facilitate studies follicle oocyte development in vitro and regenerative medicine. To investigate pluripotency human amniotic fluid (hAFSCs) their ability to differentiate cells, we isolated CD117(+)/CD44(+) hAFSC line showed fibroblastoid morphology intrinsically expressed both...

BackgroundNumerous studies have shown a strong correlation between disulfidptosis and various cancers. However, the expression function of RPN1, crucial gene in disulfidptosis, remain unclear context cancer.MethodsGene clinical information on lung adenocarcinoma were obtained from The Cancer Genome Atlas (TCGA) Genotype-Tissue Expression (GTEx) databases. RPN1 was analyzed using Timer2.0 Human Protein (HPA) Prognostic significance assessed Cox regression analysis Kaplan–Meier curves. Genetic...

Recently, we established a protocol for the cultivation of primary porcine oviduct epithelial cells (POEC), which promoted tissue-like morphology prolonged culture period. The present study focuses on developing this model into comprehensive, standardized system, as candidate tool reproductive toxicity testing and basic research. We cultivated POEC isolated from 25 animals in our system both 3 6 weeks systematically analyzed effects medium conditioning, supplementation with sera, duration...

In mammals, around the time of ovulation, hormonal profile dynamically changes in synchrony with reproductive events occurring oviduct, that is, sperm arrival, fertilization, and early embryo development. Extracellular vesicles (EVs) have been recently recognized as key components embryonic milieu; however, composition function oviductal EVs during this crucial period remains to be further explored. Therefore, we initially characterized from porcine fluid specifically critical ovulation...

Nonalcoholic fatty liver disease (NAFLD) affects approximately a quarter of the population worldwide, and persistent overnutrition is one major causes. However, underlying molecular basis has not been fully elucidated, no specific drug approved for this disease. Here, we identify regulatory mechanism that reveals novel function Rab2A in progression NAFLD based on energy status PPARγ. The mechanistic analysis shows nutrition repletion suppresses phosphorylation AMPK-TBC1D1 signaling, augments...

The generation of oocyte-like cells (OLCs) from stem cell differentiation in vitro provides an optimal approach for studying the mechanism oocyte development and maturation. aim this study was to investigate activation bone morphogenetic protein 15 gene (BMP15) during human amniotic fluid (hAFSCs) into OLCs. After days differentiation, OLCs with a diameter 50–60 μm zona pellucida (ZP)-like morphology were observed. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed...

Fatty liver disease is a serious health problem worldwide and the most common cause for chronic metabolic disorders. The major challenge in prevention intervention of this incomplete understanding underlying mechanism thus lack potent therapeutic targets due to multifaceted interdependent factors. In study, we investigated role signaling adaptor protein, GRB2-associated-binding protein 2 (Gab2), fatty using an animal model. Gab2 expression hepatocytes responded various factor stimulations,...

Key reproductive events such as fertilization and early embryonic development occur in the lumen of oviduct. Since investigating these processes vivo is both technically challenging ethically sensitive, cell culture models have been established to reproduce oviductal microenvironment. Compartmentalized systems, particularly air-liquid interface cultures (ALI; cells access medium only from basolateral side), result highly differentiated oviduct epithelial cultures. The oxygen (O

We have previously mimicked the morphological and functional changes occurring in oviduct epithelium during estrous cycle vitro by using an air-liquid interface (ALI) culture system basolateral application of 17β-estradiol (E2) progesterone (P4). In current study we aimed to explore transcriptomic elicited E2 P4 together simulation dissect individual effects on physiology. Primary porcine epithelial cells (POECs) (N = 6 animals) were cultured at ALI. After differentiation for 11 days,...

Objective Super-high and ultra-high spatial resolution computed tomography (CT) imaging can be advantageous for detecting temporal bone pathology guiding treatment strategies. Methods Six cadaveric specimens were used to evaluate the microanatomic structures utilizing following CT reconstruction modes: normal (NR, 0.5-mm slice thickness, 512 2 matrix), high (HR, 1,024 super-high (SHR, 0.25-mm (UHR, 2,048 matrix). Noise signal-to-noise ratio (SNR) air measured at each mode. Two observers...

Autonomic dysregulation in Parkinson's disease (PD) can precede motor deficits and is associated with reduced quality of life, progression, increased mortality. Objective markers autonomic involvement PD are limited. Corneal confocal microscopy (CCM) a rapid ophthalmic technique that quantify small nerve damage range peripheral neuropathies. Here we investigated whether CCM be used to assess symptoms PD. Based on the scale for outcomes (SCOPA-AUT), patients were classified into those without...

We recently developed an air-liquid interface long-term culture of differentiated bovine oviductal epithelial cells (ALI-BOEC). This ex vivo oviduct epithelium is capable supporting embryo development in co-culture up to the blastocyst stage without addition medium. However, rates were markedly lower than conventional vitro production procedures. In present study, we assessed target gene expression ALI-BOEC derived embryos test their similarity from culture. screened previously published...

Ovarian steroid hormones (mainly E2 and P4) regulate oviduct physiology. Serum-E2 acts on the epithelium from basolateral cell compartment. Upon ovulation, apical compartment of is temporarily exposed to follicular fluid, which contains much higher levels than serum. The aim this study was evaluate effects human periovulatory fluid epithelial cells using two porcine in vitro models.A line derived oviductal (CCLV-RIE270) characterized (lineage markers, proliferation characteristics...