A5011954696- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Circular RNAs in diseases
- NF-κB Signaling Pathways
- Genetic and phenotypic traits in livestock
- Epigenetics and DNA Methylation
- T-cell and Retrovirus Studies
- Psoriasis: Treatment and Pathogenesis
- Vector-Borne Animal Diseases
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
Centre National de la Recherche Scientifique
2014-2023
Université de Lorraine
2019-2023
Center for Integrated Protein Science Munich
2012-2019
Ingénierie Moléculaire et Physiopathologie Articulaire
2019
Ludwig-Maximilians-Universität München
2012-2019
Institut Pasteur
2014
National Education and Research Network
2009-2010
In placental mammals, inactivation of one the X chromosomes in female cells ensures sex chromosome dosage compensation. The 17 kb non-coding Xist RNA is crucial to this process and accumulates on future inactive chromosome. most conserved region, A contains eight or nine repeats separated by U-rich spacers. It implicated recruitment late inactivated genes silencing compartment likely complex PRC2. Little known about structure region more generally structure. Knowledge its restricted an NMR...
Highlights•MLE binds with preference to a conserved stem-loop structure in roX RNA•ATP-dependent unwinding of the stem creates an alternative MLE-roX structure•RNA remodeling is prerequisite for selective MSL2 interaction•Remodeling may constitute switch initiate assembly MSL-DCCSummaryDosage compensation Drosophila involves global activation genes on male X chromosome. The activating complex (MSL-DCC) consists male-specific-lethal (MSL) proteins and two long, noncoding RNAs. RNAs are...
Abstract In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, such as dimethylated H3K9 (H3K9me2) HP1. Here, we show that JIL-1’s targeting depends on a PWWP domain-containing protein JASPer (JIL-1 Anchoring Stabilizing Protein). JASPer-JIL-1 (JJ)-complex major form of in vivo targeted genes telomeric transposons via binding domain H3K36me3 nucleosomes,...
Random X-chromosome inactivation ensures dosage compensation in mammals through the transcriptional silencing of one two X chromosomes present each female cell. Silencing is initiated differentiating epiblast mouse embryos coating nascent inactive chromosome by non-coding RNA Xist, which subsequently recruits Polycomb Complex PRC2 leading to histone H3-K27 methylation. Here we examined ES cells early steps transition from naive towards stem as a model for inducing vitro. We show that these...
Long non-coding RNAs have emerged as critical regulators of cell homeostasis by modulating gene expression at chromatin level for instance. Here, we report that the lncRNA ANRIL, associated with several pathologies, binds to thousands loci dispersed throughout mammalian genome sharing a 21-bp motif enriched in G/A residues. By combining ANRIL genomic occupancy transcriptomic analysis, established list 65 and 123 genes potentially directly activated silenced trans, respectively. We also found...
Abstract In flies, the chromosomal kinase JIL-1 is responsible for most interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from acquiring heterochromatic marks, like dimethylated H3K9 (H3K9me2) HP1. Here, we show that JIL-1’s targeting depends on a new PWWP domain-containing protein JASPer (JIL-1 Anchoring Stabilizing Protein). The JASPer-JIL-1 (JJ)-complex major form of in vivo targeted genes telomeric transposons via binding domain H3K36me3...