To study the mechanism of acquired resistance to bortezomib, a new antitumor drug that is first therapeutic proteasome inhibitor, we established series bortezomib-resistant T lymphoblastic lymphoma/leukemia cell lines, designated JurkatBs, from parental Jurkat line via repeated selection. There were no significant differences in growth curves or colony formation between JurkatB cells and cells. The effects bortezomib on cytotoxicity, cycle arrest, induction apoptosis decreased compared with...

Cognitive behavior therapy (CBT) is effective for the treatment of Internet gaming disorder (IGD). However, mechanisms by which CBT improves IGD-related clinical symptoms remain unknown. This study aimed to discover therapeutic mechanism in IGD subjects using resting-state functional magnetic resonance imaging (rsfMRI). Twenty-six and 30 matched healthy controls (HCs) received rsfMRI scan assessments; 20 completed then were scanned again. The amplitude low-frequency (ALFF) values...

Oxygen is an essential substance for the maintenance of human life. It also widely used in clinical and diving medicine. Although oxygen crucial survival, too much can be harmful. Excessive inhalation a short period time lead to injury, lung one main target organs. Acute injury (ALI) induced by hyperbaric (HBO) notably more severe than that caused normobaric oxygen, yet systematic research on such its regression scarce. In this study, two independent experiments were designed. first...

Statins, which are 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase competitive inhibitors, not only lower blood cholesterol but also exert pleiotropic and beneficial effects in various diseases. However, the of statins on acute lung injury (ALI) induced by hyperbaric oxygen (HBO) have been investigated. The present study is first to investigate simvastatin ALI HBO 8- 9-wk-old C57BL/6 mice exposed 0.23 MPa [=2.3 atmosphere absolute (ATA)] hyperoxia (≥95% O2) for 6 h. Mice were...

In murine allogeneic transplantation models, ICOS gene-transduced bone marrow-derived mesenchymal stem cells (MSCs ICOS-EGFP ) were evaluated for their effects on GvHD severity and long-term survival. Lethally irradiated BALB/c or first filial generation of C57BL/6 (CB6F1) mice transplanted with marrow splenocytes from to establish acute models. Recipient injected MSCs , MSCs, EGFP ICOS-Ig fusion protein, + ICOS-Ig, PBS (control group). Long-term survival, rates severity, CD4 T-cell...

Phenobarbital (PB) promotes liver tumorigenesis in rodents, part through activation of the constitutive androstane receptor (CAR) and consequent changes hepatic gene expression increases hepatocyte proliferation. A typical effect CAR by PB is a marked induction <i>Cyp2b10</i> liver; latter has been suspected to be vital for PB-induced hepatocellular This hypothesis was tested here using <i>Cyp2a(4/5)bgs</i>-null (null) mouse model which all <i>Cyp2b</i> genes are deleted. Adult male female...

Abstract The effect of variations in the expression cytochrome P450 reductase (CPR or POR) is determined mice with decreased POR to identify potential vulnerabilities people low expression. There an age-dependent appearance increasing vacuolization proximal tubules renal cortex 4- 9-month-old male (but not female) Cpr-low (CL) mice. These have all cells body and upregulation lysosome-associated membrane protein 1 cortex. Vacuolization also seen extrahepatic CL extrarenal mice, but...

Objective: The objective of this study was to explore whether a single deep helium-oxygen (heliox) dive affects physiological function. Methods: A total 40 male divers performed an open-water heliox 80 m seawater (msw). diving time 280 min, and the breathing 20 min. Before after dive, blood saliva samples were collected, cell counts, cardiac damage, oxidative stress, vascular endothelial activation, hormonal biomarkers assayed. Results: An msw induced significant increase in percentage...

This study sought to investigate therapeutic effects of hydrogen-rich saline (HRS) combined with hyperbaric oxygen (HBO2) in an experimental rat model acute lung injury (ALI).Forty male Sprague-Dawley rats were randomly divided into sham, LPS, LPS + HBO2, HRS, and HBO2 HRS groups. After intratracheal injection LPS-induced ALI, the given a single-agent or treatment. The treatments continued for three days this ALI. At end experiment, pathological, inflammatory factors, cell apoptosis...

Nuclear factor kappa B (NF-κB) is the critical transcriptional in pathogenesis of acute lung injury (ALI). NF-κB regulates expression changes inflammatory factors such as tumor necrosis alpha (TNF-α), interleukin-1β (IL-1β) and interleukin 6 (IL-6). In a previous study we showed that decompression sickness (DCS) caused by simulated unsafe fast buoyancy ascent escape (FBAE) could result ALI, which was characterized rat tissue. The purpose present work to roles TNF-α process DCS-induced FBAE....

Recent studies have demonstrated that peroxisome proliferator-activated receptor-beta/delta (PPAR-β/δ) has a protective effect during lung injury induced by bleomycin and polymicrobial sepsis, but its function in pulmonary oxygen toxicity is unknown. In this study, we used GW0742, PPAR-β/δ agonist, GSK0660, antagonist, to test the role of due hyperbaric (HBO2) exposure. Lung was rats HBO2 exposure (2.3 ATA, 100%O2, 8 hours). Sixty male Sprague-Dawley were randomly divided into 6 groups:...

ABSTRACT Hyperbaric oxygen (HBO) therapy is extensively used to treat a number of ailments. Although crucial for survival, too much can be harmful. Excessive inhalation in short period time lead injury, and the lung one main target organs. Lung injury induced by hyperbaric notably more severe than that caused normobaric oxygen, yet systematic research on such its regression scarce. In this study, mice were exposed 2 atmospheres absolute (ATA), ⩾ 95% 2, 4, 6, 8 hours. Changes histopathology,...

Background: To investigate therapeutic effects of hydrogen-rich saline (HRS) combined with hyperbaric oxygen (HBO) in an experimental rat model Acute Lung Injury (ALI). Methods: 40 Male Sprague-Dawlay rats were randomly divided into sham, LPS, LPS + HBO, HRS and HBO groups. ALI was induced by intratracheal injection then the respectively given single agent treatment or treatment. The treatments continued for 3 days such ALI. At end experiment, lung pathological , inflammatory factors cell...

This study measured pulmonary function in divers after a single helium-oxygen (heliox) dive to 80, 100, or 120 metres of sea water (msw).A total 26 participated, whom 15, five, and six performed msw dive, respectively. While immersed, the breathed heliox air, then oxygen during surface decompression hyperbaric chamber. Pulmonary was twice before diving, 30 min 24 h diving.At 80 forced expiratory volume 1 s (FEV₁)/forced vital capacity (FVC) ratio maximum flow at 25% (MEF₂₅) values decreased...

Objective Oxygen is often used clinically to treat hypoxia patients,however,prolonged exposure normobaric oxygen as well hyperbaric will induce toxicity.Our study was designed explore different manifestations of pulmonary toxicity (POT) induced by prolonged and exposures.Methods Sixty male SD rats were randomly divided into 6 groups exposed 0.10、0.15、0.20、0.25 MPa 0.30 pure for 56,20,10,8 hours respectively.Rats air control.Protein levels white cell counts in the bronchoalveolar lavage fluid...