In this study, we have identified the source of nitric oxide (NO) produced in human inflammatory joints by analyzing expression inducible NO synthase. ex vivo organ cultures, both synovium and cartilage from patients with rheumatoid arthritis NO. The production was suppressed NG-monomethyl-L-arginine, an inhibitor amount correlated proportion CD14+ cells corresponding tissue (r = 0.8, P < 0.05). Immunohistochemical analysis as well situ hybridization showed that synthase predominantly...

A method is presented for explicitly representing dimensions, tolerances, and geometric features in solid models. The combines CSG boundary representations a graph structure called an object graph. Dimensions are represented by relative position operator. can automatically translate changes dimensional values into corresponding geometry topology. representation provides important foundation higher-level application programs to automate tolerance analysis synthesis. implementation of...

Abstract Although IL-10 has been implicated in the pathogenesis of several autoimmune diseases, mechanisms by which this cytokine mediates inflammatory lesions remain to be elucidated. Exocrine gland destruction is an important early step development Sjögren’s syndrome. To better understand role syndrome, we made transgenic mice mouse gene was regulated human salivary amylase promoter. Transgenic expression induced apoptosis glandular tissue and lymphocyte infiltration consisting primarily...

Abstract Objective . To investigate the expressions of costimulatory molecules CD86 (B7‐2, B70) and CD28 by cells obtained from synovial tissues (ST) fluids (SF) patients with rheumatoid arthritis (RA). Methods Monoclonal antibodies (MAb) against were used for immunochemical study synovia 18 RA patients, 4 osteoarthritis (OA) normal subjects. These MAb also flow cytometry isolated ST 8 5 OA SF mononuclear patients. Results Immunohistochemical examination revealed that CD86+ occurred in 11...

ABSTRACT A number of antibodies generated during human respiratory syncytial virus (RSV) infection have been cloned by the phage library approach. Antibodies reactive with an immunodominant epitope on F glycoprotein this a high affinity for affinity-purified antigen. These antibodies, however, much lower mature surface infected cells and are nonneutralizing. In contrast, potent neutralizing antibody has protein but purified or in viral lysates. The data indicate that at least two immunogens...

To investigate the possibility of an immune response to retroviral antigens or detecting retrovirus in Sjögren's syndrome.Retroviruses were sought labial salivary glands and peripheral blood mononuclear cells from patients with syndrome by immunoblotting assay, immunohistochemical polymerase chain reaction (PCR), reverse transcriptase (RT) activity transmission electron microscopy.Sera five 15 (33%) reacted against p24 group specific antigen (gag) human immunodeficiency virus (HIV). Labial...

The current study examined the ability of a new adsorbent, CTR, to remove enterotoxins, toxic shock syndrome toxin-1 (TSST-1), and cytokines from blood and/or serum in vitro effects extracorporeal treatment with CTR column on mortality rate inflammatory responses endotoxic vivo.Laboratory investigation.University company experimental laboratory.CTR is composed porous cellulose beads which hydrophobic organic compound hexadecyl alkyl chain has been covalently bound surface as ligand....

AIM: To determine whether transforming growth factor-beta 1 (TGF-beta 1) has a pathogenetic role in disease of the salivary glands. METHODS: An indirect immunohistochemical technique was used to analyse TGF-beta expression six specimens normal gland and 23 surgical specimens. RESULTS: strongly expressed ductal epithelial cells tissues (six cases) inflammatory conditions (eight 11 cases). In contrast, not detectable expressing HLA-DR around infiltrating CD4+ CD45RO+ activated T cells, tissue...

Endovascular treatment (ET) is widely used for thrombotic complications of vascular access (VA) hemodialysis. We evaluated the effectiveness both ET and surgical interventions complications.We studied 533 patients who underwent procedures (a total 879 procedures) 54 received 156 VA obstruction (endovascular group; group 1). The were further divided into two groups: balloon-angioplasty (the thrombus was surgically removed stenotic lesions dilated by balloon angioplasty; 189 procedures; 2)...

Background: Although percutaneous transluminal angioplasty has been established as a first-line therapy for access failure in dialysis, there are few reports on primary patency after angioplasty. We investigated factors associated with following the first performed vascular construction patients arteriovenous fistula, including blood flow volume before and previously reported factors. Methods: used medical records at six dialysis centers to retrospectively identify analyze prognostic 159...

The intracellular cation contents were determined in isolated perfused rat heart using cobaltic EDTA as a marker of the extracellular space. In hearts which Na+ accumulation was induced with monensin, ionophore, during 20 min-ischemia otherwise did not result Na+, levels and Ca2+ significantly higher after reperfusion significant decrease K+. While recovery cardiac mechanical function (CMF) complete control hearts, incomplete monensin-hearts. Dichlorobenzamil (DCB), most specific inhibitor...

In Japan, the number of patients receiving dialysis is 314,180 at end 2013 and 97% are treated with hemodialysis. And mean age percentage diabetes have been increasing. For this reason, preparations a new vascular access (VA) its long-term maintenance become difficult. guidelines by Japanese Society for Dialysis Therapy (JSDT), endovascular treatment (ET) positioned as first line VA stenosis. The procedure ET itself very simple. revision health insurance set an expensive technical fee in...

Summary The characteristics of the immunity induced by viral antigens or conferred antiviral antibody via different routes administration were evaluated comparatively. C57BL/6 mice immunized intranasal, intradermal enteric with a live recombinant vaccinia virus expressing respiratory syncytial (RSV) F glycoprotein (F·rVV) RSV, and then challenged intranasally RSV. Inhibition RSV replication was observed in lungs all mice; however, only intranasal immunization hindered nose. Lung...

Antihypertensive effects of chronic oral administration adrenergic β- blocking agents were assessed in SHR. Propranolol, pindolol, oxprenolol, atenolol and labetalol used as β-blockers the these compounds on blood pressure heart rate compared with those hydralazine, a representative vasodilating antihypertensive agent. oxprenolol produced definite decrease rate; development hypertension was retarded. Pindolol only after longer period such associated insignificant rate. With shorter drug an...

Radiofrequency Ablation Combined With Chemoembolization: Treatment of Recurrent Hepatocellular Carcinomas After HepatectomyHaruyuki Takaki1, Koichiro Yamakado1, Hiroshi Sakurai2, Atsuhiro Nakatsuka1, Katsuya Shiraki3, Syuji Isaji2 and Kan Takeda1Audio Available | Share

Heating of thebaine with dil. hydrochloric acid, followed by addition 4-amino-antipyrine and potassium ferricyanide, basification this mixture ammonia results in red coloration, the color changing to reddish brown on heating 100°. The limit detection spot reaction is 4γ/0.05cc. Presence morphine, codeine, narcotine, papaverine does not interfere as these alkaloids do undergo coloration. By utilization coloration reaction, colorimetric determination can be made presence papaverine. procedure...

Abstract Cell-assisted lipotransfer (CAL) is an advanced lipoinjection method that uses autologous with addition of a stromal vascular fraction (SVF) containing adipose-derived stem cells (ASCs). The CAL procedure manual isolation from fat requires cell processing to be performed in clean environment. To isolate without the need for center, such as operation theater, we developed novel SVF using closed washing concentration device (CCD) hollow fiber membrane module. CCD consists sterilized...

目的:血液透析患者におけるそう痒症の最近の実態を明らかにするとともに,オピオイドκ受容体作動薬ナルフラフィン塩酸塩の臨床効果を検討することを目的とした.方法:東海地区17施設の血液透析患者を対象に,かゆみの程度[5段階カテゴリー評価(白取のかゆみスコア)およびvisual analogue...

Abstract Objectives Availability of cord blood ( CB ) processing has been limited by the need for electrically aided centrifugal techniques, which often produce only low final cell product yield. Here, we describe development and characterization a novel filter device aimed at allowing processing, using gentle gravity‐led flow. Materials methods was processed with (CellEffic , consisting non‐woven fabric), without any centrifugation. Cells were harvested flushing either HES or physiological...

Examinations ware made on the analysis of morphine by ceric ammonium nitrate-2, 4-dinitrophenylhydrazine method. The limit detection this method is 1.5γ/0.05cc. Thebaine and papaverine interfere in determination both must be removed a simple separation procedure. Narcotine did not affect resulting data but there fear its having caused secondary reaction, so that was also examined. procedure for same as described codeine, with nitrate 0.4% solution period reaction at 20 minutes. When codeine...