A5074592517- Protease and Inhibitor Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Research on Leishmaniasis Studies
- Neonatal Respiratory Health Research
- Reproductive System and Pregnancy
- Virology and Viral Diseases
- Infant Nutrition and Health
- Multiple Sclerosis Research Studies
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- Plant Virus Research Studies
- Trypanosoma species research and implications
- Cardiac, Anesthesia and Surgical Outcomes
- Cell Adhesion Molecules Research
- Sex and Gender in Healthcare
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- Steroid Chemistry and Biochemistry
- Influenza Virus Research Studies
- Hemostasis and retained surgical items
- Medicinal Plants and Bioactive Compounds
- Pregnancy and preeclampsia studies
- Infective Endocarditis Diagnosis and Management
- Lipoproteins and Cardiovascular Health
- Lysosomal Storage Disorders Research
University of Ferrara
2013-2024
Arcispedale Sant'Anna
2012
In this study, we employed a sensitive activity assay system to measure cerebrospinal fluid (CSF) and serum levels of active matrix metalloproteinase-9 (MMP-9) in 37 relapsing-remitting (RR), 15 secondary progressive (SP) nine primary (PP) multiple sclerosis (MS) patients, grouped according clinical magnetic resonance imaging (MRI) evidence disease activity. We also studied, as neurological controls, 48 patients with other inflammatory disorders (OIND) non-inflammatory (NIND). To assess...
Matrix Metalloproteases (MMPs) and cytokines have been involved in the pathogenesis of multiple sclerosis (MS). However, no studies still explored possible associations between two families molecules. The present study aimed to evaluate contribution active MMP-9, MMP-2, interleukin- (IL-) 17, IL-18, IL-23, monocyte chemotactic proteins-3 MS interconnections MMPs cytokines. proteins were determined serum cerebrospinal fluid (CSF) 89 patients 92 other neurological disorders (OND) controls....
Background Excessive production of matrix metalloproteinase 9 (MMP-9) is linked to tissue damage and anastomotic leakage after large bowel surgery. Hence, the aim this study was verify whether different strategies fluids administration can reduce MMP-9 expression. Methods In in vitro experiment, authors tested hypothesis a direct inhibition by used perioperatively, i.e., lactated Ringer's solution, 3.4% poligeline, hydroxyethyl starch 130/0.4. vivo 36 patients undergoing surgery for colon...
Background Little is known about the involvement of matrix metalloproteinase-2 (MMP-2) and its tissue inhibitor TIMP-2 in multiple sclerosis (MS). Objective To elucidate actual implication MMP-2 MS. Methods Cerebrospinal fluid (CSF) serum levels active were measured by activity assay system ELISA, respectively, 67 patients with relapsing–remitting MS (RRMS), categorized according clinical magnetic resonance imaging (MRI), 129 controls. Results MMP-2/TIMP-2 ratio mean values an intrathecal...
Administration of normal saline might increase circulating levels pro-inflammatory cytokines and may cause variation plasmatic electrolytic hyperchloremic acidosis, which in turn can impair renal function. Hence the use balanced solutions could influence inflammatory cascade triggered by surgical procedures, electrolyte concentration, acid-base equilibrium, function.This is a double blind randomized trial. Forty patients undergoing major abdominal surgery (bowel cancer) were allocated two...
Background: The activity of matrix metalloproteinase-9 (MMP-9) depends on two isoforms, an 82 kDa active MMP-9 modulated by its specific tissue inhibitor (TIMP-1), and a 65 TIMP-1 resistant MMP-9. relevance these enzymatic isoforms in multiple sclerosis (MS) is still unknown. Objective: To investigate the contribution to MS pathogenesis. Methods: We measured serum levels assay systems 86 relapsing–remitting (RRMS) patients, categorized according clinical magnetic resonance imaging (MRI)...
While Biochemistry textbooks for University degree courses do not deal with oligosaccharides, this subject is now becoming essential in Biomedical Sciences several reasons. Oligosaccharides are currently used foods and beverages their useful physicochemical prebiotic qualities, albeit excessive consumption may have side effects. They also components of milk, where they exert a protective role on infants acting as stimulants the development immune system gut microbiota. seen an alternative to...
Introduction The accurate distinction between periprosthetic joint infections (PJI) and aseptic failures (AF) is of paramount importance due to differences in treatment. However, this could be challenging by using the current criteria. Various synovial fluid biomarkers are being assessed improve diagnostic accuracy. Myeloperoxidase (MPO), an enzyme contained granules neutrophils, may a promising biomarker for PJI. Methods Synovial fluids 99 patients ( n = 65 PJI according EBJIS criteria; 34...
COVID-19 brought back to the attention of scientific community that males are more susceptible infectious diseases. What is clear for other infections—that sex and gender differences influence both risk infection mortality—is not yet fully elucidated plague, particularly bubonic although this knowledge can help find specific defences against a disease which vaccine available. To address question, we analysed data on plague from hospitals in different parts world since early eighteenth...
Background: Oxidative stress (OxS) has been linked to several pregnancy-related complications. Previous studies demonstrated that lactoferrin (LF) the ability modulate inflammation, OxS and immune function. Therefore, we aimed observe whether vaginal LF administration was able decrease in amniotic fluid (AF) of pregnant women undergoing midtrimester genetic amniocentesis. Methods: In this open-label clinical study, 60 were divided into three groups: CONTROLS (n=20) not treated with LF; LACTO...
Journal Article Matrix metalloproteinase-9 activity detected in body fluids is the result of two different enzyme forms Get access Tiziana Bellini, Bellini 1Section Biochemistry and Clinical Biochemistry, Department Molecular Biology, University Ferrara, Via Luigi Borsari 46, 2Section Neurology, Medical Surgical Sciences Communication Behaviour, 3Section Anesthesia Intensive Care, Surgical, Anesthesiological Radiological Science, 4Neuroradiology Unit, Neurosciences Rehabilitation, Azienda...
Several studies showed that hydroxyethyl starch (HES), a synthetic colloid used in volume replacement therapies, interferes with leukocyte-endothelium interactions. Although still unclear, the mechanism seems to involve inhibition of neutrophils’ integrin. With aim provide direct evidence binding HES neutrophils and investigate influence on neutrophil chemotaxis, we isolated treated cells different concentrations fluorescein-conjugated (HES-FITC), or without stimuli...
Inflammation plays an important role in pregnancy, and cytokine matrix metalloproteases (MMPs) imbalance has been associated with premature rupture of membranes increased risk preterm delivery. Previous studies have demonstrated that lactoferrin (LF), iron-binding protein anti-inflammatory properties, is able to decrease amniotic fluid (AF) levels IL-6. Therefore, we aimed evaluate the effect vaginal LF administration on PGE 2 level MMP-TIMP system women undergoing genetic amniocentesis. One...
The relevance of human leukocyte antigen (HLA)-G in dimeric form multiple sclerosis (MS) is still unknown.To investigate the contribution cerebrospinal fluid (CSF) HLA-G dimers MS pathogenesis.CSF amounts 78-kDa were measured by western blot analysis 80 relapsing-remitting (RRMS) patients and 81 inflammatory 70 non-inflammatory controls.CSF 78 kDa more frequent RRMS than (p<0.01) controls (p<0.001) magnetic resonance imaging (MRI) inactive MRI active (p<0.00001).Our findings suggest that may...
Background . Natalizumab is a highly effective treatment approved for multiple sclerosis (MS). The opening of the blood-brain barrier mediated by matrix metalloproteinases (MMPs) considered crucial step in MS pathogenesis. Our goal was to verify utility serum levels active MMP-2 and MMP-9 as biomarkers twenty patients treated with Natalizumab. Methods Serum specific tissue inhibitors TIMP-1 TIMP-2 were determined before 21 months therapy. Results did not differ during treatment. ratio...
Introduction: Statin therapy is often associated with muscle complaints and increased serum creatine kinase (CK). However, although essential in determining damage, this marker not specific for skeletal muscle. Recent studies on animal models have shown that slow fast isoforms of troponin I (ssTnI fsTnI, respectively) can be useful markers injury. The aim study was to evaluate the utility ssTnI fsTnI as monitor statin-induced damage. Materials methods: A total 51 patients (14 using 37...
We investigated the effect of balanced (BS) and unbalanced (UBS) solutions in absence or presence hydroxyethyl starch (HES) on neutrophil functionality, evaluating release matrix metalloproteinase (MMP)-9, myeloperoxidase (MPO), MMP-8. Neutrophils were isolated by gradient centrifugation dextran sedimentation incubated BS UBS without with HES, Interleukin-8 (IL-8) Lipopolysaccharide (LPS). MMP-9, MPO, MMP-8 assayed commercially available ELISA kits. There was not any influence volume...
The domain structure of hemagglutinin‐neuraminidase from Sendai virus (cHN) was investigated by studying the thermal stability in 20–100°C range. Differential scanning calorimetry evidences two conformational transitions. first transition is apparently a reversible two‐state process, with T m 48.3°C, and shifted to 50.1°C presence substrate analogue 2,3‐dehydro‐2‐deoxy‐ N ‐acetyl neuraminic acid, meaning that binding involved transition. second transition, apparent 53.2°C, accompanied...