Abstract Pyroptosis, a form of programmed cell death (PCD), has garnered increasing attention as it relates to innate immunity and diseases. However, the involvement pyroptosis in mechanism by which lobaplatin acts against colorectal cancer (CRC) is unclear. Our study revealed that treatment with reduced viability HT-29 HCT116 cells dose-dependent manner. Morphologically, treated exhibited microscopic features swelling large bubbles emerging from plasma membrane, transmission electron...

Intestinal ischemia-reperfusion injury occurs in acute intestinal obstruction, intussusception, mesenteric artery embolism, and other diseases can lead to local necrosis, distant organ involvement, or systemic reactions, with high morbidity mortality. Ferroptosis plays a crucial role injury, inhibition of ferroptosis may provide new approaches for treating the disease. SIRT3 protects cells from oxidative stress be involved process ferroptosis. We hypothesized that resveratrol, an agonist...

Abstract Caudal-related homeobox transcription factor 2 (CDX2), an intestine-specific nuclear factor, has been strongly implicated in the tumourigenesis of various human cancers. However, functional role CDX2 development and progression colorectal cancer (CRC) is not well known. In this study, knockdown colon cells promoted cell proliferation vitro, accelerated tumor formation vivo, induced a cycle transition from G0/G1 to S phase, whereas overexpression inhibited proliferation....

Bile acids serve a critical role in the induction of gastric intestinal metaplasia (IM) and carcinogenesis. The present study investigated effects bile on IM formation. results demonstrated that expression levels caudal‑related homeobox transcription factor 2 (CDX2), mucin (MUC2) farnesoid X receptor (FXR) were increased vitro vivo following treatment with acids, CDX2 transcriptionally activated MUC2 expression. Furthermore, knockdown FXR attenuated acid‑enhanced promoter activity protein...

The present study aimed to investigate the role of matrix metalloproteinase‑1 (MMP‑1) in development colorectal cancer and reveal mechanism underlying this progression. Bioinformatics methods a public dataset were first used analyze MMP‑1 gene expression dataset. patients was assessed by immunohistochemistry; its association with clinicopathological parameters significance for prognosis analyzed. Then proliferation, scratch Transwell assays xenograft model assess change malignant behavior...

Abstract Farnesoid X receptor (FXR, encoded by NR1H4), a critical regulator of bile acid homeostasis, is widely implicated in human tumorigenesis. However, the functional role FXR colorectal cancer (CRC) and precise molecular mechanism remain unclear. In this study, we demonstrated that expression was downregulated colon tissues decreased predicted poor prognosis. Knockdown promoted cell growth invasion vitro, facilitated xenograft tumor formation distant metastasis vivo, whereas ectopic had...

Repeated and long-term oxaliplatin therapy leads to drug resistance severe adverse events, which limit its clinical use. These difficulties highlight the importance of identifying potent specific combinations enhance antitumor effects oxaliplatin. The farnesoid X receptor (FXR) deficiency in colorectal cancer (CRC) suggests that restoring FXR function might be a promising strategy for CRC treatment. A combination study showed GW4064 acted synergistically with colon cells. plus inhibited cell...

Aberrant expression of microRNA-155 (miR-155) has been reported in several human cancers and is associated with prognosis patients. However, the clinical significance miR‑155 its underlying mechanisms involved hepatocarcinogenesis remain to be determined. In this study, we demonstrated that miR-155 was elevated both hepatocellular carcinoma (HCC) tissues cell lines. Clinical association analysis revealed high correlated malignant clinicopathological characteristics including large tumor...

Abstract Farnesoid X receptor (FXR, encoded by NR1H4), a bile acid‐activated nuclear receptor, is widely implicated in human tumorigenesis. The FXR agonist obeticholic acid (OCA) has preliminarily displayed tumour suppressor potential. However, the anticancer effects of this agent on colorectal cancer (CRC) remain unclear. In study, treatment colon cells with OCA inhibited cell proliferation and invasion vitro, retarded growth vivo prevented G 0 /G 1 to S phase transition. Moreover,...

Abstract The complex interaction between tumor-associated macrophages (TAMs) and tumor cells through several soluble factors signaling is essential for colorectal cancer (CRC) progression. However, the molecular mechanism involved remains elusive. In this study, we demonstrated that MMP1 derived from TAMs markedly facilitated colon cell proliferation via accelerating cycle transition G0/G1 to S G2/M phase. Moreover, exogenous activated cdc25a/CDK4-cyclin D1 p21/cdc2-cyclin B1 complexes...

The gasdermins (GSDMs) family is proposed to be pore-forming effector proteins that cause cell membrane permeabilization and pyroptosis. Despite our increasing knowledge of GSDMD, GSDME GSDMB, the biological functions regulation GSDM expression activation remain elusive for most GSDMs. In this study, we analyzed molecular characteristics oncogenic role genes systematically.TCGA, CCLE, cBioPortal, GEPIA, CellMiner BioGRID databases were utilized in study. Immunohistochemical analysis a series...

Fourier transform infrared (FT-IR) spectroscopy is an effective tool for investigation of chemical changes at the molecular level. We previously demonstrated that FT-IR can reliably distinguish multiple types carcinoma from healthy tissue. Because various stomach diseases are common, it important to explore a noninvasive and rapid method detect malignancy gastritis in endoscopic biopsies. Our aim was classify biopsies into healthy, gastritis, through use spectroscopy.A total 103 samples,...

Colon cancer is associated with a high incidence and poor prognosis. The aim of the present study was to determine whether Fourier transform infrared (FTIR) microspectroscopy can be used monitor chemotherapy drug‑induced apoptosis SW620 colon cells. 50% inhibitory concentration (IC50) 5‑fluorouracil (5‑FU), main chemotherapeutic agent for treatment colorectal cancer, determined as inhibition growth cells using an MTT assay. Cell starvation 5‑FU synergized arrest in G1 S phases cell cycle....

Postoperative intra-abdominal adhesions are a major complication after abdominal surgery. Although various methods have been used to prevent and treat adhesions, the effects not satisfactory. Emodin, naturally occurring anthraquinone derivative an active ingredient in traditional Chinese herbs, exhibits variety of pharmacological effects. In our study, we demonstrated effect emodin treatment on preventing postoperative adhesion formation.A total 48 rats were divided into six groups....

Tumor cell invasion and metastasis are important processes in colorectal cancer that exert negative effects on patient outcomes; consequently, a prominent topic the field of study is identification safe affordable anticancer drugs against metastasis, with limited side effects. Ginkgolic acid phenolic extracted from ginkgo fruit, exotesta leaves. Previous studies have indicated ginkgolic inhibits tumor growth number types cancer; however, considered colon cancer. In present study, SW480 cells...

Gastric cancer (GC) has high morbidity and mortality is a serious threat to public health. The flavonoid compound vitexin known exhibit anti-tumor activity. In this study, we explored the therapeutic potential of in GC its underlying mechanism.The viability, migration, invasion cells were determined using MTT, scratch wound healing, transwell assays, respectively. Target molecule expression was by western blotting. Tumor growth liver metastasis evaluated vivo nude mice. Protein tumor tissues...

// Lei Zhou 1, 2, 3 , Zhixin Wang 4 Xinsen Xu 2 Yong Wan Kai Qu Haining Fan Qiangpu Chen 1 Xuejun Sun Chang Liu Department of Hepatobiliary Surgery, The Affiliated Hospital Binzhou Medical University, Binzhou, China First Hospital, School Medicine, Xi’an Jiaotong Xi’an, General Hepatopancreatobiliary Qinghai Xining, Correspondence to: Chen, e-mail: drcqp@hotmail.com Sun, sunxj@mail.xjtu.edu.cn Liu, liuchangdoctor@163.com Keywords: Nek7, hepatocellular carcinoma, cell...