A major challenge in cardiac tissue engineering is the host's immune response to artificial materials. To overcome this problem, we established a scaffold-free system for assembling cell constructs using an automated Bio-3D printer. This printer has previously been used fabricate other three-dimensional (3D) constructs, including liver, blood vessels, and cartilage. In present study, tested function vivo of tubular construct fabricated system. Cardiomyocytes derived from induced pluripotent...

Osteoarthritis (OA) is a major joint disease in humans and many other animals. Consequently, medical countermeasures for OA have been investigated diligently. This study was designed to examine the regeneration of articular cartilage subchondral bone using three-dimensional (3D) constructs adipose tissue-derived mesenchymal stem cells (AT-MSCs).

Osteoarthritis is a leading cause of pain and joint immobility, the incidence which increasing worldwide. Currently, total replacement only treatment for end-stage disease. Scaffold-based tissue engineering promising alternative approach repair but subject to limitations such as poor cytocompatibility degradation-associated toxicity. To overcome these limitations, completely scaffold-free Kenzan method bio-3D printing was used fabricate cartilage constructs feasible repairing large chondral...

ABSTRACT Osteoarthritis is a major joint disease that has been extensively investigated in humans and model animals. In this study, we examined the regeneration of articular cartilage subchondral bone using artificial scaffold‐free constructs composed adipose tissue‐derived mesenchymal stem cells (AT‐MSCs) created bio three‐dimensional (3D) printing with needle‐array. Printed were implanted into osteochondral defects right femoral trochlear groove six mini‐pigs, left femurs as controls....

Abstract Cardiac constructs fabricated using human induced pluripotent stem cells-derived cardiomyocytes (iPSCs-CMs) are useful for evaluating the cardiotoxicity of and cardiac response to new drugs. Previously, we scaffold-free three-dimensional (3D) tubular a bio-3D printer, which can load spheroids onto needle array. In this study, developed method measure contractile force evaluate drug in constructs. Specifically, measured movement tip upon contraction on The beating rate were evaluated...

Cryopreservation is a method used for preserving living cells by cooling them to very low temperatures. Although cryopreservation methods oocytes and embryos have been developed use in reproductive medicine, there are no established yet cell aggregates (spheroids) regenerative medicine. We bio-three-dimensional (3D) printer that can fabricate scaffold-free 3D constructs loading spheroids onto needle array. fabricated several such as blood vessels, liver, diaphragm, conduit nerves using this...

In end-stage osteoarthritis (OA), osteochondral defects reach the subchondral bone and cartilage tissue of sufficient thickness is required to compensate for defects. Adipose-derived mesenchymal stem/stromal cells (ADSCs), which are abundant in body, have potential differentiate into may be a useful cell source regeneration. If it possible fabricate ADSC-derived constructs that can cover damaged area, this could lead development new regenerative therapy OA replace currently available...

Abstract Ultrathin gold oxide (AuOx) films were patterned on surfaces using UV/O3 treatment applied through quartz photomasks with Cr patterns. The formation of AuOx was confirmed reflective spectroscopy and X-ray photoelectron spectroscopy. Reflective conducted antireflective (AR) multilayer substrates demonstrated a minimum reflectance at 535 nm, which shifted by 3.0 nm toward longer wavelengths upon formation. This shift enabled the imaging patterns camera. maximum contrast reached 9.3%...

Osteochondral lesion is a major joint disease in humans. Therefore, this study was designed to investigate the regeneration of articular cartilage and subchondral bone, using three-dimensional constructs autologous adipose tissue-derived mesenchymal stromal cells without any biocompatible scaffolds. Mesenchymal were harvested by liposuction from seven pigs, isolated enzymatically, expanded until construct creation. The pig models had two osteochondral defects (cylindrical with diameter 5.2...

Abstract Background Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and with scaffold-free three-dimensional (3D) constructs equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies expanded until over 1.0 × 10 7 at passage 2 (P2). Results SM-MSCs were strongly positive CD11a/CD18, CD44, major histocompatibility complex (MHC) class I; moderately CD90,...

Osteoarthritis is a major joint disease for which medical interventions have been extensively investigated in humans and animals. In this study, we examined the regeneration of articular cartilage subchondral bone using scaffold-free construct consisting adipose tissue-derived mesenchymal stem cells (AT-MSCs) fabricated bio three-dimensional (3D) printer. AT-MSCs were isolated from three rabbits cultured to number sufficient creation 3D-printed constructs. One consisted 960 spheroids...

Abstract Morbidity and mortality rates associated with atherosclerosis-related diseases are increasing. Therefore, developing new research models is important in furthering our understanding of atherosclerosis investigate novel treatments. Here, we designed vascular-like tubular tissues from multicellular spheroids composed human aortic smooth muscle cells, endothelial fibroblasts using a bio-3D printer. We also evaluated their potential as model for Mönckeberg’s medial calcific sclerosis....

We hypothesized that semi-quantitative determinations of changeable blood flows in granulation and scar tissues during tendon healing could be helpful for differentiation between the acute phase rich vessels remodeling with fewer vessels. Ten Thoroughbreds injured superficial digital flexor (SDFT) a forelimb were used evaluation tendons racehorses using color Doppler (CD) ultrasonography. Using longitudinal CD images, features maximum activities defined. These rhythmically blinking, tiny to...

The development of bio-three-dimensional (bio-3D) printers has led to significant advances in regenerative medicine. Three-dimensional constructs, including spheroids, are maintained by extracellular matrix proteins secreted cells so that the can be cultured conditions closer physiological environment. This study aimed create a useful 3D construct as model dentin-pulp complex.

The sizes of Japanese native horses have drastically decreased, and protection these populations is important for horse culture. Social trials as well scientific attempts are necessary maintaining the breed. Mesenchymal stem cells (MSCs) potential a cell source various therapies. However, there been no reports on MSCs horses. We aimed to isolate characterize from horse, Noma horse. Plastic-adherent self-replicating were isolated horse's peripheral blood (PB). had trilineage surface antigen...

Background: Although autogenous bone implantation is considered to be the gold standard for reconstruction of defects, this approach remains challenging when treating extensive defects (EBDs). Therefore, artificial materials (AMs) such as and scaffolds are often used EBDs. Nevertheless, complications material failure, foreign body reaction, infection common. To overcome these issues, we aimed develop a new treatment an EBD using scaffold-free adipose-derived stromal cells (ADSCs) fabricate...

Dedifferentiated fat (DFAT) cells have been shown to be multipotent, similar mesenchymal stem (MSCs). In this study, we aimed establish and characterize equine DFAT cells. Equine adipocytes were ceiling cultured, then dedifferentiated into by the seventh day of culture. The number was increased over 10 million fourth passage. Flow cytometry showed that strongly positive for CD44, CD90, major histocompatibility complex (MHC) class I; moderately CD11a/18, CD105, MHC II; negative CD34 CD45....

Mesenchymal stem cells (MSCs) are adult multipotent that capable of self-renewal and differentiation into multiple cell lineages. Methods for therapy using MSCs have been developed in equine medicine. Recently, human dental pulp (DPSCs) drawn much attention owing to their trophic factor producing ability minimally invasive collection methods. However, there no reports on pulp-derived (eDPCs). Therefore, the aim this study was isolate characterize eDPCs from discarded wolf teeth....

A 12-year-old neutered male shih tzu developed progressive pelvic limb paraparesis. Computed tomography showed a radiolucent mass lesion in the spinal canal at left side of 11th thoracic vertebra. The was not enhanced by intravenous contrast medium injection. It hyperintense on both T1- and T2-weighted magnetic resonance images. signal intensity decreased with fat suppression technique, indicating fatty origin. After removal via T11–T12 hemilaminectomy, chronic panniculitis confirmed...

Aim of this study was to evaluate the relationships exercise and tendon injury with Doppler flows appearing in superficial digital flexor (SDFT) young Thoroughbreds during training periods. The forelimb SDFTs 24 one- two-year-old clinically free any orthopaedic disorders were evaluated using grey-scale (GS) color (CD) images two periods between December 2013 April 2015. Twelve horses per year examined December, February, that began September ended April. SDFT 3 longitudinal equal lengths...

Synovium-derived mesenchymal stromal cells (SM-MSCs) from seven Thoroughbreds with naturally occurring intra-articular fracture proliferated to over ten million by the second passage. Using three experimental Thoroughbreds, columnar osteochondral defects were made arthroscopically at bilateral distal radius. Five allogenic SM-MSCs implanted into right defect, and another five injected radio-carpal joint (implantation site). No left defect or same (control At 3 6 weeks after surgery,...