A5008280433- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Aortic Disease and Treatment Approaches
- Mechanical Circulatory Support Devices
- Infective Endocarditis Diagnosis and Management
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac Ischemia and Reperfusion
- Aortic aneurysm repair treatments
- Magnesium Alloys: Properties and Applications
- Cardiac and Coronary Surgery Techniques
- Aluminum Alloys Composites Properties
- Lung Cancer Diagnosis and Treatment
- Aluminum Alloy Microstructure Properties
- Congenital Heart Disease Studies
- Tissue Engineering and Regenerative Medicine
- Transplantation: Methods and Outcomes
- Thyroid Disorders and Treatments
- Tracheal and airway disorders
- Gear and Bearing Dynamics Analysis
- Lubricants and Their Additives
- Cardiovascular Function and Risk Factors
- Pulmonary Hypertension Research and Treatments
- Tribology and Lubrication Engineering
- Lung Cancer Treatments and Mutations
- Pericarditis and Cardiac Tamponade
Mitsui Memorial Hospital
2019-2024
National Kyushu Medical Center
2008-2023
Teikyo University Hospital
2017-2023
Junshin Gakuen University
2023
European Organisation for Research and Treatment of Cancer
2022
Clinical Research Institute
2020-2022
The Japanese Society of Gastroenterological Surgery
2021
National Hospital Organization
2006-2020
Saga University
2010-2019
The University of Tokyo
1997-2019
Background Small caliber vascular prostheses are not clinically available because synthetic lack endothelial cells which modulate platelet activation, leukocyte adhesion, thrombosis, and the regulation of vasomotor tone by production vasoactive substances. We developed a novel method to create scaffold-free tubular tissue from multicellular spheroids (MCS) using "Bio-3D printer"-based system. This system enables creation pre-designed three-dimensional structures computer controlled robotics...
A major challenge in cardiac tissue engineering is the host's immune response to artificial materials. To overcome this problem, we established a scaffold-free system for assembling cell constructs using an automated Bio-3D printer. This printer has previously been used fabricate other three-dimensional (3D) constructs, including liver, blood vessels, and cartilage. In present study, tested function vivo of tubular construct fabricated system. Cardiomyocytes derived from induced pluripotent...
The aim of our study was to develop a completely scaffold-free, viable, contractile cardiac tissue capable being grafted into the damaged native heart.Our technology is based on fundamental characteristics self-assembling nature cells. We created spheroids by plating mixture rat neonatal ventricular cardiomyocytes, human dermal fibroblasts, and coronary microartery endothelial cells in ultralow attachment plates. First, optimal cell ratios for 3 sources were determined. Next, approximately 1...
Aims Malignant mesothelioma is a highly aggressive cancer that usually diagnosed at advanced stages; thus, sensitive and specific markers are necessary for its early definitive diagnosis. The aim of this study was to evaluate the diagnostic utility prognostic significance BAP 1 EZH 2 in malignant mesothelioma. Methods results expression investigated by immunohistochemistry 32 mesotheliomas 44 benign mesothelial proliferative lesions, including well‐differentiated papillary ( n = 4),...
Cancer-associated fibroblasts (CAFs) interact closely with cancer cells, supporting their growth and invasion. To investigate the role of microRNA-21 (miR-21) in lung adenocarcinoma, especially its CAF component, situ hybridisation was applied to samples from 89 invasive adenocarcinoma cases. MiR-21 expression observed both cells CAFs. When patients were stratified by expression, miR-21 levels CAFs (n = 9), but not 21), inversely correlated patient survival; miR-21high exhibited lower...
Abstract Before they are used in the clinical setting, effectiveness of artificially produced human-derived tissue-engineered medical products should be verified an immunodeficient animal model, such as severe combined mice. However, small models not sufficient to evaluate large-sized for human use. Thus, large model is necessary order properly efficacy products, artificial grafts. Here we report development pig operational (OIDP), by surgically removing thymus and spleen, creating a...
Low-grade lung adenocarcinoma of fetal type, which is well characterized by its unique clinicopathologic and molecular features, recognized as a distinct variant cancer. In contrast, high-grade with lung-like morphology (HG-LAFM) has not been studied widely. To characterize this subset better, we analyzed 17 adenocarcinomas at least focal component resembling developing epithelium in the pseudoglandular phase lung. These rare (ca. 0.4%) carcinomas occurred predominantly elderly men heavy...
This study aimed to clarify claudin-6 expression patterns in cancers.We surveyed by immunohistochemistry using tissue microarray 860 tumours, including germ cell tumours and major carcinomas. Claudin-6 was expressed consistently (28 of 28, 100%), whereas only 64 (8%) 832 non-germ demonstrated expression. Further immunohistochemical full sections diffuse staining all seminomas (n=14), embryonal carcinomas (n=10), yolk sac (n=12) mononuclear trophoblastic cells choriocarcinomas (n=3), focal...
Thyroid epithelial cells (thyrocytes) in autoimmune thyroid disease have been found to express DR antigens on their surfaces, and interferon-gamma (IFN gamma) induces antigen expression. This study was undertaken determine the effect of IFN gamma response human thyrocytes TSH stimulation relationship between expression induced by gamma, using monolayer cultures Graves' thyrocytes. When confluent thyrocyte monolayers were incubated with or Bu2cAMP (DBcAMP) for 7-9 days, T3 thyroglobulin...
The Japanese registry for mechanical assisted circulatory support (J-MACS) is a prospective to collect all data of implantable left ventricular assist device (LVAD) (and part paracorporeal VAD) established in 2010. first analytical report was published 2017. organization running J-MACS used be the pharmaceuticals and medical devices agency (PMDA), but has been changed council clinical use related academic societies 2017.Since 2018, we methods as follows: first, eliminated VAD from analysis....
We have recently demonstrated that fasudil, a Rho-kinase inhibitor, is effective in suppressing coronary artery spasm patients with vasospastic angina. Thus, blockade of may provide novel therapeutic strategy to treat ischemic syndrome caused by the spasm. Severe still remains life-threatening serious complication bypass grafting (CABG). In this study, we examined inhibitory effect fasudil intractable severe after CABG. Three who underwent CABG showed myocardial ischemia resistant intensive...
We have previously shown that insulin-like growth factor (IGF-I) suppresses basal and GHRH-induced GH gene transcription. cAMP is a putative intracellular mediator of GHRH action. We, therefore, studied the mechanism IGF-I action on somatotroph with or without activators. Primary rat pituitary cells growing in serum-free medium were treated IGF-I. secretion was measured by RIA, mRNA levels hybridization to [32P]GH cDNA. 8-Bromo-cAMP (8-Br-cAMP; 0.625 mM) stimulated after 72 h 238%. (6.5 nM)...
Abstract. This preliminary study was undertaken to investigate the efficacy of high-dose iv methylprednisolone pulse therapy in 5 patients with Graves' ophthalmopathy. One gram sodium succinate given daily for 3 successive days. The 3-day infusion repeated 7 times at intervals 1 week; total duration weeks. clinical improvement eye involvements by assessed immediately after last therapy. assessment effect ophthalmopathy showed a good response patients, fair one, and no one. However, one...
Spread through air space (STAS) is recognized as a pattern of invasion in lung adenocarcinoma and has been reported to be predictor recurrence survival patients with early-stage adenocarcinoma. However, this parameter not studied well stage III (N2) In study, we evaluated the association between STAS patterns adenocarcinoma.We retrospectively reviewed data from 76 at University Tokyo who underwent surgery August 1998 December 2013. Statistical analysis was performed determine impact...
Abstract. It has been reported that cytokines, especially interleukin 1 and interferon-γ, inhibit the thyroid hormone secretion gene expression of human peroxidase thyroglobulin. Interleukin 6 recently found to be an important cytokine for regulation immunoendocrine interaction intrathyroidal production reported. Therefore, we investigated messenger RNA by in thyrocytes clarify further functional role glands. Thyrocytes dispersed from Graves' tissues were incubated with TSH or without 6. (5...
Abstract Background Pre‐resection pleural lavage cytology is useful to predict tumor recurrence and the prognosis of lung cancer patients. Recently, extracellular vesicles (EVs) isolated from effusion specimens have come under spotlight, several studies showed that microRNA in EVs associated with prognosis. MicroRNA‐21 (miR‐21) a representative onco‐microRNA, miR‐21 (EV‐miR‐21) promotes dissemination by inducing mesothelial mesenchymal transition (MMT) peritoneal cavity. In this study, we...