A5101524163- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Advanced biosensing and bioanalysis techniques
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Advanced Nanomaterials in Catalysis
- Cerebrovascular and Carotid Artery Diseases
- Nanopore and Nanochannel Transport Studies
- Nanoplatforms for cancer theranostics
- Biosensors and Analytical Detection
- Metabolomics and Mass Spectrometry Studies
- Medical Imaging and Analysis
- RNA Interference and Gene Delivery
- Erythrocyte Function and Pathophysiology
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- MXene and MAX Phase Materials
- Gynecological conditions and treatments
- Salivary Gland Disorders and Functions
- ATP Synthase and ATPases Research
- Sphingolipid Metabolism and Signaling
- Folate and B Vitamins Research
- Pregnancy and preeclampsia studies
- Photonic and Optical Devices
- SARS-CoV-2 detection and testing
Zhengzhou Business University
2024
University of Electronic Science and Technology of China
2024
Zhengzhou University
2017-2023
BGI Group (China)
2020
State Council of the People's Republic of China
2019
Henan Provincial People's Hospital
2016
University of Chinese Academy of Sciences
2012-2015
Changchun Institute of Applied Chemistry
2012-2015
Chinese Academy of Sciences
2012-2015
Chinese PLA General Hospital
2011
The cell membrane plays a key role in compartmentalization, nutrient transportation and signal transduction, while the pattern of protein distribution at both cytoplasmic ectoplasmic sides remains elusive. Using combination single-molecule techniques, including atomic force microscopy (AFM), single molecule spectroscopy (SMFS) stochastic optical reconstruction (STORM), to study structure nucleated membranes, we found that (1) proteins side form dense layer (4 nm) on top lipid bilayer; (2)...
Hypoglycemic sulfonylureas stimulate insulin release by binding to a regulatory subunit of plasma membrane ATP-sensitive K+ (K(ATP)) channels. The consequent closure K(ATP) channels leads depolarization, opening voltage-dependent Ca2+ channels, influx, and rise in intracellular [Ca2+]. Recently, however, it has been suggested that may have an additional action on secretion, independent changes [Ca2+] but dependent the activity protein kinase C (PKC). We investigated mechanisms involved...
An <italic>in situ</italic> detection of plasma exosomal microRNA for lung cancer diagnosis using duplex-specific nuclease and molybdenum disulfide nanosheets.
We developed a novel, effective, reliable and convenient TRCLIA method for the simultaneous detection of CEA NSE in serum.
The aim of this study is to establish a new method with high sensitivity, accuracy, and stability for the determination human IgG then expand it analyze severe acute respiratory syndrome corona virus 2 (SARS-CoV-2)-specific IgM IgG, which great significance screening diagnosis COVID-19. In study, magnetic Fe
We utilized force tracing to directly record the endocytosis of single gold nanoparticles (Au NPs) with different sizes, revealing size-dependent dynamics and crucial role membrane cholesterol. The force, duration velocity Au NP invagination are accurately determined at single-particle microsecond level unprecedentedly.
We used in situ atomic force microscope (AFM) to explore the exquisite structure of mitochondrial membranes under quasi-native conditions. The outer surface membrane is slightly rough and protein-embedded inside, while inner smooth intermembrane space protein-covered on matrix side.
Background: As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to complex composition biological samples, results from combined detection CEA VEGF are often taken as comprehensive indicator order make an accurate judgment on disease. However, most current methods can only be used detect content one biomarker. Therefore, it is necessary explore simple,...
Relying on fluorophore-conjugated aptamers, we revealed the high expression and clustered distribution of globo H cancer cell membranes uncovered potential interactions between cancer-associated carbohydrates<italic>via</italic>super-resolution imaging.
In this study, an interesting phenomenon was found where cells (including tumor and normal cells) managed to significantly enhance chemiluminescence (CL) signals. The possible reaction mechanism may be that can severely damaged by CL substrates, the released contents, possibly proteins (such as cytochrome c), remarkably magnify owing increased production of singlet oxygen. More importantly, based on above phenomena, a novel cell-assisted enhanced strategy proposed for rapid label-free...
This paper reports improved miR-21 detection signals based on a PGM combined with probe self-assembly and β-CD. The novel biosensor has been successfully applied to detect in serum samples.
Abstract The occurrence of many diseases is closely related to the high expression DNA methyltransferase 1 (DNMT1). However, most studies are focused on detection DNMT1 activity, a few concerned with content. In this study, we developed simple and highly sensitive chemiluminescence (CL) assay for method, anti‐DNMT1 monoclonal antibody was coated polystyrene microplate capture DNMT1. Then polyclonal goat anti‐rabbit immunoglobulin G horseradish peroxidase (IgG‐HRP) were respectively added...
The cell membrane provides critical cellular functions that rely on its elaborate structure and organization. of turtle membranes is an important part ongoing study erythrocyte membranes. Using a combination atomic force microscopy single-molecule spectroscopy, we characterized the with molecular resolution in quasi-native state. High-resolution images both leaflets revealed smooth outer leaflet protein covered inner leaflet. This asymmetry was verified by which detects numerous exposed...
The smooth outer leaflet and protein-covered inner of chicken erythrocyte membranes are observed by atomic force microscopy under near-native conditions.