A5038529581- Photodynamic Therapy Research Studies
- Photoacoustic and Ultrasonic Imaging
- Optical Imaging and Spectroscopy Techniques
- Nanoplatforms for cancer theranostics
- bioluminescence and chemiluminescence research
- Ultrasound and Hyperthermia Applications
- Lung Cancer Diagnosis and Treatment
- Luminescence Properties of Advanced Materials
- Medical Imaging Techniques and Applications
- Optical Coherence Tomography Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Peripheral Artery Disease Management
- Cancer, Hypoxia, and Metabolism
- Diabetic Foot Ulcer Assessment and Management
- Infrared Thermography in Medicine
- Medical and Biological Ozone Research
- Pain Management and Treatment
- Gastric Cancer Management and Outcomes
- Gallbladder and Bile Duct Disorders
- Solid State Laser Technologies
- Laser Applications in Dentistry and Medicine
- Pediatric Hepatobiliary Diseases and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Laser Design and Applications
- Cerebrovascular and Carotid Artery Diseases
Prokhorov General Physics Institute
2013-2024
Russian Academy of Sciences
2015
The study is aimed at developing a method for monitoring photodynamic therapy (PDT) of tumor using chlorin-type photosensitizers (PSs). Lack chlorin e6 (Cе6) photobleaching, hemoglobin oxygenation and blood flow during light exposure can limit the PDT effectiveness. Phototheranostics includes spectral-fluorescence diagnostics Ce6 distribution in NIR range with simultaneous assessment flow. Fluorescence were performed single laser λexc=660±5 nm. Combined spectroscopic allowed estimation...
Emission spectral properties and quantum efficiency of upconversion particles NaYF4, SrF2, LaF3, BaF2 и CaF2, doped with rare earth ions pair Yb3+–Er3+ were studied using continuous wave (CW) pulsed periodic excitation modes in the near infrared (NIR) range. Analysis obtained results showed that intensity ratio luminescence green red ranges depends on pulse duration. Thus, by changing duration can be controlled. Crystals higher phonon energy are more sensitive to change pumping mode....
The motivation for the present study was need to develop methods of urgent intraoperative biopsy during surgery removal intracranial tumors. Based on experience previous joint work GPI RAS and N.N. Burdenko National Medical Research Center Neurosurgery introduce fluorescence spectroscopy into clinical practice, an approach combining various optical-spectral techniques, such as autofluorescence spectroscopy, 5-ALA induced protoporphyrin IX, diffuse reflection broadband light, which can be...
Hypoxia is a characteristic feature of many tumors. It promotes tumor proliferation, metastasis, and invasion can reduce the effectiveness types cancer treatment.
To reduce the frequency of relapses after surgical removal a brain tumor, it is critically important to completely remove all affected areas without disrupting functionality vital organs. Therefore, intraoperative differential diagnostics micro-areas tumor tissue with their subsequent or destruction an urgent task that determines success operation as whole. Optical spectroscopy has shown its advantages over past decade when used tool for metabolic navigation. And one most promising options...
The difficulty of intraoperative delineation glial tumors is due to the peculiarities their growth along vessels and nerve fibers with infiltration healthy white matter. Insufficiently complete removal tumor tissues leads recurrences, excessive fraught neurological complications. Optical spectroscopy methods are characterized by high speed, accuracy non-invasiveness, which determines prospects use for demarcation boundaries such tumors. Fluorescence diffuse reflectance have found wide...
Upconversion nanoparticles have attracted considerable attention as luminescent markers for bioimaging and sensing due to their capability convert near-infrared (NIR) excitation into visible or NIR luminescence. However, the wavelength of about 970 nm is commonly used upconversion luminescence excitation, where strong absorption water observed, which can lead laser-induced overheating effects. One strategies avoiding such heating involves shifting shorter wavelengths region. influence change...
Non-invasive detection of polarized macrophages in tumors is an urgent task terms combined antitumor therapy. By analyzing the fluorescence lifetime metabolic cofactors—the reduced form nicotinamide adenine dinucleotide (NADH) and flavins—differences cellular metabolism normal tissue, tumor, inflammatory anti-inflammatory were demonstrated. In this work we studied changes polarization obtained from THP-1 monocytes response to photodynamic therapy with 5-aminolevulinic acid (ALA–PDT)....
Determination of the iron oxide nanoparticles (IONPs) local temperature during laser heating is important in aspect phototherapy. We have carried out theoretical modeling IONPs electromagnetic (EM) field enhancement and under action near individual ensembles with different sizes, shapes chemical phases. For experimental determination temperature, we used fluorescence thermometry rhodamine B (RhB) based on its lifetime. Depending shape their location space, a significant change spatial...
Upconversion nanoparticles are promising for many applications. For triple-doped (NPs), the luminescence intensity shows a non-linear dependence on rare-earth ion concentration, making it difficult to obtain bright phosphors with high energy output. We investigated transfer processes in β-NaGdF4:Yb-Er-Tm NPs and considered strategies increasing thulium intensity, particular, use of core–shell structures. The spectra were analyzed short-wavelength infrared (SWIR) visible (VIS) regions. Er3+...
The data about absorption spectra properties of the photosensitized tumor tissues in vivo are very important for evaluation photosensitizer concentration and influence chemical environment on tissue. This information will help one appropriate choosing irradiation light dose wavelength photodynamic therapy (PDT) treatment. interaction with tissue may change its spectrum. Moreover during PDT treatment can also affect structure hence simple technique based measurement reflection by means fiber...
Two Bispectral contrast enhancement approaches for the fluorescence diagnosis with chlorine-e6 and a wide field-of-view imaging system excitation at 405 nm time-resolved background suppression were analyzed compared.
We developed a system for the analysis of spatial distribution photosensitizers (PS) based on tetrapyrrole compounds in tissues retina. Tetrapyrrole were chosen because most them are characterized by strong absorption tissue transparency window. Calibration curves determining concentration PS different methods constructed. Registration PS's fluorescence consists two optical channels. First channel single point spectroscopic technique is used to determine exact at certain field. Second...
РезюмеКатионные бактериохлорины перспективны как антимикробные фотосенсибилизаторы для антибактериальной фотодинамической терапии.Настоящая работа посвящена изучению свойств нового наноструктурированного катионного фотосенсибилизатора на основе циклодекстриновой дисперсии производного бактериохлорина -метилового эфира 13 3 -N-(N-метилникотинил)бактериопурпуринимида (КБХ), с целью оптимизации состава и выбора интервала времени от введения до проведения терапии инфицированных гнойных...
Light sources based on light-emitting diodes (LED) can be created for almost any wavelength suitable exciting photosensitizers (PS). This paper presents the main approaches to develop LED-based light well-controlled photodynamic exposure and results of their implementation. High power density LED were designed tested both in vitro vivo studies, as well irradiation various pathological foci during therapy.
Currently, the most promising method for study of pharmacokinetics drugs with fluorescent properties is spectral-fluorescent method. In this article, we propose an algorithm expanding dynamic range spectrum analyzer by automatically monitoring maximum spectral density in recorded fluorescence and controlled changes accumulation time depending on value, followed compensation output signal regard to change, as well hardware circuit solutions that allow algorithm. Testing LESA-01-"Biospeс"...