A5101400891- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Phagocytosis and Immune Regulation
- Immune responses and vaccinations
- Influenza Virus Research Studies
- RNA Interference and Gene Delivery
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- DNA and Nucleic Acid Chemistry
- Viral gastroenteritis research and epidemiology
- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- CAR-T cell therapy research
- Diabetes and associated disorders
- Extracellular vesicles in disease
- Eosinophilic Esophagitis
- vaccines and immunoinformatics approaches
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- 2D Materials and Applications
- Escherichia coli research studies
Peter Doherty Institute
2016-2025
The University of Melbourne
2016-2025
State Key Laboratory of Respiratory Disease
2019-2024
Guangzhou Medical University
2019-2024
First Affiliated Hospital of Guangzhou Medical University
2019-2022
University of Oxford
2020
Medawar Building for Pathogen Research
2020
Fuzhou University
2018-2019
Mucosal associated invariant T (MAIT) cells recognise conserved microbial metabolites from riboflavin synthesis. Striking evolutionary conservation and pulmonary abundance implicate them in antibacterial host defence, yet their functions protection against clinically important pathogens are unknown. Here we show that mouse Legionella longbeachae infection induces MR1-dependent MAIT cell activation rapid accumulation of with immune detectable immunocompetent animals. is more evident mice...
Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. MAIT also activated during viral infections, yet it remains unknown whether play a significant protective or even detrimental role infections vivo. Using murine experimental challenge with two strains of influenza A virus, we show that accumulate early infection, upregulation CD25, CD69 Granzyme...
Abstract Mucosal-associated invariant T (MAIT) cells are activated by unstable antigens formed reactions of 5-amino-6- D -ribitylaminouracil (a vitamin B2 biosynthetic intermediate) with glycolysis metabolites such as methylglyoxal. Here we show superior preparations in dimethylsulfoxide, avoiding their rapid decomposition water ( t 1/2 1.5 h, 37 °C). Antigen solution structures, MAIT cell activation potencies (EC 50 3–500 pM), and chemical stabilities described. Computer analyses antigen...
A new siRNA delivery platform based on black phosphorus nanosheets is constructed which shows excellent therapy efficiency for tumors.
ICOS- and IL-23–mediated costimulation are important for driving in vivo activation of antigen-specific MAIT cells.
Skin-resident CD8+ T cells include distinct interferon-γ-producing [tissue-resident memory type 1 (TRM1)] and interleukin-17 (IL-17)-producing (TRM17) subsets that differentially contribute to immune responses. However, whether these populations use common mechanisms establish tissue residence is unknown. In this work, we show TRM1 TRM17 navigate divergent trajectories acquire residency in the skin. depend on a T-bet-Hobit-IL-15 axis, whereas develop independently of factors. Instead, c-Maf...
Even though black phosphorus (BP) has exhibited outstanding capabilities in biomedical, physical, and energy fields, the issues of degradation under ambient conditions unreactive functional interface limit its further application. There are numerous methodologies utilized to prevent BP degradation; however, these methods usually generate problems normally do not involve alterations chemically inert BP. Herein, for first time, we propose a simple efficient strategy prepare modify nanosheets...
Abstract Mucosal-associated Invariant T (MAIT) cells are recognized for their antibacterial functions. The protective capacity of MAIT has been demonstrated in murine models local infection, including the lungs. Here we show that during systemic infection mice with Francisella tularensis live vaccine strain results evident cell expansion liver, lungs, kidney and spleen peripheral blood. responding manifest a polarised Th1-like MAIT-1 phenotype, transcription factor cytokine profile, confer...
Mucosal-associated invariant T (MAIT) cells detect microbial infection via recognition of riboflavin-based antigens presented by the major histocompatibility complex class I (MHC-I)–related protein 1 (MR1). Most MAIT in human peripheral blood express CD8αα or CD8αβ coreceptors, and binding site for CD8 on MHC-I molecules is relatively conserved MR1. Yet, there no direct evidence interacting with MR1 functional consequences thereof. Similarly, role lymphocyte function remains ill-defined....
Abstract Mucosal-associated invariant T (MAIT) cells produce inflammatory cytokines and cytotoxic granzymes in response to by-products of microbial riboflavin synthesis. Although MAIT are protective against some pathogens, we reasoned that they might contribute pathology chronic bacterial infection. We observed proximity Helicobacter pylori bacteria human gastric tissue, so, using MR1-tetramers, examined whether gastritis a mouse H. SS1 infection model. Following infection, accumulated high...
Mucosal-associated invariant T (MAIT) cells are known for their rapid effector functions and antibacterial immune protection. Here, we define the plasticity of interferon-γ (IFN-γ)–producing MAIT1 interleukin-17A (IL-17A)–producing MAIT17 cell subsets in vivo. Whereas T-bet + remained stable all experimental settings, after adoptive transfer or acute Legionella Francisella infection, RORγt could undergo phenotypic functional conversion into both MAIT1/17 − cells. This ensured that played a...
Abstract Mucosal‐associated invariant T (MAIT) cells are a major subset of innate‐like mediating protection against bacterial infection through recognition microbial metabolites derived from riboflavin biosynthesis. Mouse MAIT egress the thymus as two main subpopulations with distinct functions, namely, T‐bet‐expressing MAIT1 and RORγt‐expressing MAIT17 cells. Previously, we reported that inducible T‐cell costimulator interleukin (IL)‐23 provide essential signals for optimal MHC‐related...
Abstract Cell death mechanisms in T lymphocytes vary according to their developmental stage, cell subset and activation status. The control of mucosal-associated invariant (MAIT) cells, a specialized population, are largely unknown. Here we report that MAIT cells express key necroptotic machinery; receptor-interacting protein kinase 3 (RIPK3) mixed lineage domain-like (MLKL) protein, abundance. Despite this, discovered the loss RIPK3, but not effector MLKL or apoptotic caspase-8,...
DNA nanostructures with controllable motions and functions have been used as flexible scaffolds to precisely spatially organize molecular reactions at the nanoscale. The construction of dynamic site-specifically incorporated functional elements is a critical step toward building nanomachines. Artificial self-assembled also developed mimic key biological processes like various small biomolecule- protein-based biochemistry pathways. Here, we report trident-shaped (TS DNA) nanoactuator, in...
Abstract This unit describes the utility of various mouse models infection and immunization for studying mucosal‐associated invariant T (MAIT) cell immunity: MAIT cells can be isolated from lungs (or other tissues/organs) then identified characterized by flow cytometry using MR1 tetramers in combination with a range antibodies. The response kinetics, cytokine profiles, functional differentiation lung are studied following bacterial pathogen Legionella longbeachae or Salmonella enterica...
Abstract Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. MAIT also activated during viral infections, yet it remains unknown whether play a significant protective or even detrimental role infections vivo . Using murine experimental challenge with two strains of influenza A virus, we show that accumulated were early infection, upregulation...
MAIT cells are abundant, highly evolutionarily conserved innate-like lymphocytes expressing a semi-invariant T cell receptor (TCR), which recognizes microbially derived small intermediate molecules from the riboflavin biosynthetic pathway. However, in addition to their TCR-mediated functions they can also be activated TCR-independent manner via cytokines including IL-12, -15, -18, and type I interferon. Emerging data suggest that expanded by range of viral infections, significantly...
Introduction Mucosal-associated invariant T (MAIT) cells are a population of innate-like cells, which mediate host immunity to microbial infection by recognizing metabolite antigens derived from riboflavin synthesis presented the MHC-I-related protein 1 (MR1). Namely, potent MAIT cell antigens, 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil (5-OP-RU) and 5-(2-oxoethylideneamino)-6-D-ribitylaminouracil (5-OE-RU), form via condensation precursor 5-amino-6-D-ribitylaminouracil (5-A-RU) with...
Summary IL-23 signaling plays a key role in the pathogenesis of chronic inflammatory and infectious diseases, yet cellular targets pathways affected by this cytokine remain poorly understood. We show that receptors are expressed on large majority human MAIT, but not conventional T cells. Protein transcriptional profiling at population single cell level demonstrates stimulation with or structurally related IL-12 drives distinct functional profiles, revealing high plasticity MAIT IL-23,...