A5011943118- Monoclonal and Polyclonal Antibodies Research
- Parkinson's Disease Mechanisms and Treatments
- Systemic Lupus Erythematosus Research
- Food Allergy and Anaphylaxis Research
- Cellular transport and secretion
- Allergic Rhinitis and Sensitization
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Salivary Gland Disorders and Functions
- T-cell and B-cell Immunology
- Blood groups and transfusion
- Immunodeficiency and Autoimmune Disorders
- Endoplasmic Reticulum Stress and Disease
- Erythrocyte Function and Pathophysiology
- Heparin-Induced Thrombocytopenia and Thrombosis
- Insect and Pesticide Research
- Genetic Neurodegenerative Diseases
- Chronic Lymphocytic Leukemia Research
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Mass Spectrometry Techniques and Applications
- Pancreatic function and diabetes
- Amyotrophic Lateral Sclerosis Research
- Diabetes and associated disorders
- Protein Kinase Regulation and GTPase Signaling
Flinders University
2016-2025
The Royal Melbourne Hospital
2022
University of Utah
2022
Stanford University
2022
Charles University
2022
Flinders Medical Centre
2003-2020
The University of Adelaide
2020
South Australia Pathology
2020
University of Miami
2017
Women's and Children's Hospital
1998-2011
A comprehensive, unbiased inventory of synuclein forms present in Lewy bodies from patients with dementia was carried out using two-dimensional immunoblot analysis, novel sandwich enzyme-linked immunosorbent assays modification-specific antibodies, and mass spectroscopy. The predominant modification α-synuclein is a single phosphorylation at Ser-129. In addition, there set characteristic modifications that are to lesser extent, including ubiquitination Lys residues 12, 21, 23 specific...
Significance The aggregation of α-synuclein (aSyn) is a pathological hallmark Parkinson’s disease. Here we show that the enzymatic component innate inflammation system, known as caspase-1, hydrolyzes aSyn, rendering it aggregation-prone.
Peroxiredoxin 6 is an antioxidant enzyme and the 1-cys member of peroxiredoxin family. Using two-dimensional electrophoresis Western blotting, we have shown for first time that, in human control brain tissue patient's with Alzheimer's disease (AD), this exists as three major five minor forms pIs from 5.3 to 6.1. specific cellular markers, that present astrocytes very low levels neurons, but not detectable microglia or oligodendrocytes. In brains, there was a level staining confined "halo"...
To evaluate urinary neurotrophin receptor p75 extracellular domain (p75ECD) levels as disease progression and prognostic biomarkers in amyotrophic lateral sclerosis (ALS).The population this study comprised 45 healthy controls 54 people with ALS, 31 of whom were sampled longitudinally. Urinary p75ECD was measured using an enzyme-linked immunoassay validation included intra-assay inter-assay coefficients variation, effect circadian rhythm, stability over time at room temperature, 4°C,...
Altered neuronal connectivity in peri-infarct tissue is an important contributor to both the spontaneous recovery of neurological function that commonly develops after stroke and improvements have been induced by experimental treatments animal models. Microglia astrocytes are primary determinants environment hence strongly influence potential for plasticity. However, specific roles these cells timing critical changes their not well understood. Minocycline can protect against ischemic damage...
While the unique symbiotic relationship between anemonefishes and sea anemones is iconic, it still not fully understood how can withstand thrive within venomous environment of their host anemone. In this study, we used a proteotranscriptomics approach to elucidate proteinaceous toxin repertoire from most common anemone, Entacmaea quadricolor. Although 1251 different or toxin-like RNA transcripts were expressed in E. quadricolor tentacles (0.05% gene clusters, 1.8% expression) 5375 proteins...
Early diagnosis of lysosomal storage disorders (LSDs), before the onset irreversible pathologies, will be a key factor in development effective therapies for many these disorders. Newborn screening offers potential mechanism early detection From studies both normal and LSD-affected human skin fibroblasts we identified lysosome-associated membrane protein LAMP-1 as diagnostic marker. We have developed sensitive method quantification this with time-resolved fluorescence immunoassay. A soluble...
Objective biomarkers for amyotrophic lateral sclerosis would facilitate the discovery of new treatments. The common neurotrophin receptor p75 is up regulated and extracellular domain cleaved from injured neurons peripheral glia in sclerosis. We have tested hypothesis that urinary levels serve as a biomarker both human motor SOD1G93A mouse model disease. was identified urine patients by an immuno-precipitation/western blot procedure confirmed mass spectrometry. An ELISA established to measure...
To comparatively analyse the aberrant affinity maturation of antinuclear and rheumatoid factor (RF) B cell repertoires in blood tissues patients with Sjögren's syndrome (SjS) using an integrated omics workflow.Peptide sequencing anti-Ro60, anti-Ro52, anti-La RF was combined repertoire analysis at DNA, RNA single level subsets, affected salivary gland extranodal marginal zone lymphomas mucosa-associated lymphoid tissue (MALT) SjS.Affected contained clones as a small part polyclonal...
Peanut allergy affects 1%-3% of children in Western countries. Boiling peanuts has been demonstrated to result a hypoallergenic product that may provide safer way inducing desensitization peanut-allergic patients by first tolerance boiled peanut. We aimed assess the efficacy and safety oral immunotherapy (OIT) using sequential doses followed roasted for treating peanut children.In this open-label, phase 2, single-arm clinical trial, aged 6-18 years with positive history skin prick test ≥ 8...
RCAN1 is a chromosome 21 gene that controls secretion in endocrine cells, regulates mitochondrial function, and sensitive to oxidative stress. Regulator of calcineurin 1 (RCAN1) also an endogenous inhibitor the protein phosphatase calcineurin, inhibition which leads hypoinsulinemia diabetes humans mice. However, presence or role insulin-secreting β-cells its potential pathogenesis unknown. Hence, aim this study investigate identify β-cell function. expressed mouse islets cytosol pancreatic...
Sea cucumbers are prolific producers of a wide range bioactive compounds. This study aimed to purify and characterize one class compound, the saponins, from viscera Australian sea cucumber Holothuria lessoni. The saponins were obtained by ethanolic extraction enriched liquid-liquid partition process adsorption column chromatography. A high performance centrifugal chromatography (HPCPC) was applied saponin-enriched mixture obtain with purity. resultant purified profiled using MALDI-MS/MS...
Summary Background Current peanut oral immunotherapy is hampered by frequent adverse events. It has been shown that boiling can reduce allergenicity. Hypoallergenic products have the potential to treatment‐related reactions during desensitization. Objective To show extended (for up 12 h) progressively allergenicity while retaining T cell reactivity. Methods Raw peanuts were boiled for half, 1, 2, 4 and h in deionized water. After dehydration, raw ground, defatted soluble proteins extracted...
Vaccine-induced immune thrombocytopenia and thrombosis (VITT) is associated with antibodies that target platelet factor 4 (PF4) are heparin-independent. VITT implicated in acute, transient prothrombotic disorders triggered by adenoviral vector vaccines against coronavirus disease 2019 or adenovirus infection. We describe chronic featuring anticoagulant-refractory thromboses intermittent were VITT-like five patients (four newly reported cases the index patient). The had low levels of M...
The extrinsic efferent innervation of the distal colon and rectum guinea pig was compared, by using retrograde tracing combined with immunohistochemistry. Application carbocyanine tracer DiI to filled significantly greater numbers neurons than similar injections into colon. Approximately three-fourths all from either location were sympathetic or parasympathetic; rest spinal sensory neurons. Nerve cell bodies in prevertebral ganglia labelled two regions number. Both innervated paravertebral...
The ocular lens capsule is a smooth, transparent basement membrane that encapsulates the and composed of rigid network interacting structural proteins glycosaminoglycans. During cataract surgery, anterior routinely removed in form circular disk. We considered excised could be easily prepared for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-MSI) analysis. MALDI-MSI powerful tool to elucidate spatial distribution small molecules, peptides, within...