A5016623850- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Nanoparticles: synthesis and applications
- Electrochemical sensors and biosensors
- Carbon and Quantum Dots Applications
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Sulfur Compounds in Biology
- Oral microbiology and periodontitis research
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- Trace Elements in Health
- Mosquito-borne diseases and control
- Ferroptosis and cancer prognosis
- Alzheimer's disease research and treatments
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Enzyme Catalysis and Immobilization
- Microfluidic and Capillary Electrophoresis Applications
- Iron Metabolism and Disorders
- Immune cells in cancer
Chinese Academy of Sciences
2013-2025
Institute of Biophysics
2013-2025
Henan Academy of Sciences
2024-2025
University of Chinese Academy of Sciences
2005-2024
Zhengzhou University
2020-2024
SynZyme Technologies (United States)
2024
Yangzhou University
2017-2022
Indiana University School of Medicine
2019-2020
Google (United States)
2020
Integrated Chinese Medicine (China)
2017-2019
Abstract Nanomaterials with intrinsic enzyme-like activities (nanozymes), have been widely used as artificial enzymes in biomedicine. However, how to control their vivo performance a target cell is still challenging. Here we report strategy coordinate nanozymes tumor cells and selectively perform activity destruct tumors. We develop nanozyme using nitrogen-doped porous carbon nanospheres which possess four (oxidase, peroxidase, catalase superoxide dismutase) responsible for reactive oxygen...
Single-atom catalysts (SACs), as homogeneous catalysts, have been widely explored for chemical catalysis. However, few studies focus on the applications of SACs in enzymatic Herein, we report that a zinc-based zeolitic-imidazolate-framework (ZIF-8)-derived carbon nanomaterial containing atomically dispersed zinc atoms can serve highly efficient single-atom peroxidase mimic. To reveal its structure-activity relationship, structural evolution nanozyme (SAzyme) was systematically investigated....
The Fe3O4 nanozyme was the first reported nanoparticle with intrinsic peroxidase-like activity and has been widely used in biomedicine. To optimize its catalytic activity, we introduced histidine residues onto surface order to mimic enzymatic microenvironment of natural peroxidase enzymes. Our results show that modification a single amino acid could more than ten-fold improve apparent affinity (KM) for substrate H2O2 enhanced efficiency (kcat/KM) up twenty fold. Thus not only optimized...
As next-generation artificial enzymes, nanozymes have shown great promise for tumor catalytic therapy. In particular, their peroxidase-like activity has been employed to catalyze hydrogen peroxide (H2O2) produce highly toxic hydroxyl radicals (•OH) kill cells. However, limited by the low affinity between with H2O2 and level of in microenvironment, peroxidase usually produced insufficient •OH cells therapeutic purposes. Herein, we present a pyrite nanozyme ultrahigh affinity, resulting 4144-...
Metal–carbon hybrid materials have shown promise as potential enzyme mimetics for antibacterial therapy; however, the effects of metal states and corresponding mechanisms are largely unknown. Here, two kinds copper/carbon nanozymes were designed, with tuned copper from Cu0 to Cu2+. Results revealed that exhibited state-dependent peroxidase-, catalase-, superoxide dismutase-like activities. Furthermore, activities also primarily determined by state. The different these proposed. For...
Biofilms are surface-attached bacterial communities embedded within an extracellular matrix that create localized and protected microenvironments. Acidogenic oral biofilms can demineralize the enamel-apatite on teeth, causing dental caries (tooth decay). Current antimicrobials have low efficacy do not target protective acidic pH biofilm. Recently, catalytic nanoparticles were shown to disrupt but lacked a stabilizing coating required for clinical applications. Here, we report dextran-coated...
Single-atom nanozymes (SAzymes) represent a new research frontier in the biomedical fields. The rational design and controllable synthesis of SAzymes with well-defined electronic geometric structures are essential for maximizing their enzyme-like catalytic activity therapeutic efficacy but remain challenging. Here, melamine-mediated pyrolysis activation strategy is reported fabrication iron-based SAzyme containing five-coordinated structure (FeN5 ), identified by transmission electron...
Ferromagnetic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>, MNPs) with peroxidase-like activity enhanced oxidative cleavage of biofilm matrix components (nucleic acids, proteins and polysaccharides) in the presence H<sub>2</sub>O<sub>2</sub>, providing a novel strategy for prevention elimination, as well killing bacteria within biofilm.
Abstract Artificial peroxisome has drawn a lot of attentions for its usefulness in fabricating protocell system and great potential treating diseases. However, it is still significant challenge to prepare practicable artificial complement multiple stable functions under physiological condition. Herein, novel strategy reported design an using nanozyme accommodate enzyme‐like activities that mimics those enzymes natural peroxisome. The enzymatic active sites are introduced into graphitized...
Photoacoustic imaging (PAI) is an attractive modality, which promising for clinical cancer diagnosis due to its advantages on deep tissue penetration and fine spatial resolution. However, few tumor catalytic/responsive PAI strategies are developed. Here, we design exosome-like nanozyme vesicle in vivo H2O2-responsive of nasopharyngeal carcinoma (NPC). The intrinsic peroxidase-like activity graphene quantum dot (GQDzyme) effectively converts the 2,2′-azino-bis...
Abstract The use of natural substance to ward off microbial infections has a long history. However, the large-scale production extracts often reduces antibacterial potency, thus limiting practical applications. Here we present strategy for converting organosulfur compounds into nano-iron sulfides that exhibit enhanced activity. We show compared garlic-derived an over 500-fold increase in efficacy kill several pathogenic and drug-resistant bacteria. Furthermore, our analysis reveals hydrogen...
How to optimize the enzyme-like catalytic activity of nanozymes improve their applicability has become a great challenge. Herein, we present an l-cysteine (l-Cys) coordination-driven self-assembly strategy activate polyvinylpyrrolidone (PVP)-modified Cu single-atom MoOx-Cu-Cys (denoted as MCCP SAzymes) aiming at tumor-specific therapy. The single atom content can be rationally modulated 10.10 wt %, which activates catalase (CAT)-like MoOx nanoparticles catalyze decomposition H2O2 in acidic...
The use of catalase-like nanozymes in relieving tumor hypoxia has been a promising strategy for adjuvant radiotherapy, yet their catalytic mechanism and toxic potential remain elusive. While most studies on mechanisms focus the substrates products process, little is reported to analyze dominating surface structure activity nanozymes. Here, we ferrihydrite revealed its structure-activity relationship. Among ten main forms iron oxide nanomaterials, ferrihydrite, especially 2-line exhibited...
Nanozymes have great potential to be used as an alternative natural enzymes in a variety of fields. However, low catalytic activity compared with limits their practical use. It is still challenging design nanozymes comparable counterparts terms the specific activity. In this study, surface engineering strategy employed improve Ru using charge-transferrable ligands such polystyrene sulfonate (PSS). PSS-modified nanozyme exhibits peroxidase-like up 2820 U mg
: Intracellular bacterial survival is a major factor causing chronic or recurrent infection, leading to the failure of both host defense and/or antibiotic treatment. However, elimination intracellular bacteria challenging as they are protected from antibiotics and immune attack. Recent studies have indicated that iron helps macrophages against bacteria, contradictory traditional "nutritional immunity", in which considered key nutrient for cells. how facilitates death has not been fully...
Nanozymes, nanomaterials exhibiting enzyme-like activities, have emerged as a prominent interdisciplinary field over the past decade. To date, 1200 different been identified nanozymes, covering four catalytic categories: oxidoreductases, hydrolases, isomerases, and lyases. Catalytic activity specificity are two pivotal benchmarks for evaluating enzymatic performance. Despite substantial progress being made in quantifying optimizing of there is still lack in-depth research on preventing...
Abstract Developing strategies that emulate the killing mechanism of neutrophils, which involves enzymatic cascade superoxide dismutase (SOD) and myeloperoxidase (MPO), shows potential as a viable approach for cancer therapy. Nonetheless, utilizing natural enzymes therapeutics is hindered by various challenges. While nanozymes have emerged treatment, developing SOD-MPO in one nanozyme remains challenge. Here, we develop possessing both SOD- MPO-like activities through alloying Au Pd,...