A5038656174- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Alzheimer's disease research and treatments
- Plant tissue culture and regeneration
- Cancer-related gene regulation
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Plant Virus Research Studies
- Zebrafish Biomedical Research Applications
- Tryptophan and brain disorders
- RNA and protein synthesis mechanisms
- Dementia and Cognitive Impairment Research
- Neuropeptides and Animal Physiology
- Endoplasmic Reticulum Stress and Disease
- Nuclear Receptors and Signaling
- Nuclear and radioactivity studies
- Histone Deacetylase Inhibitors Research
- Automotive and Human Injury Biomechanics
- Polyamine Metabolism and Applications
- Transportation Safety and Impact Analysis
- Signaling Pathways in Disease
National Center for Geriatrics and Gerontology
2011-2024
Tokushima University
2003-2005
The University of Tokyo
2003-2005
Japan Science and Technology Agency
2005
RIKEN Center for Brain Science
2000
Nagoya University
1989-2000
Massachusetts General Hospital
1998-1999
University of Minnesota
1999
Harvard University
1999
National Institute for Basic Biology
1996
Most virulence (vir) genes of Agrobacterium tumefaciens that are required for the formation crown gall tumors expressed in response to such plant signal molecules as acetosyringone and lignin precursors. The phenolic signals transduced through a receptor VirA protein inner membrane bacterial cell. expression these triggers transfer specific DNA segment, called transferred (T-DNA), from Ti plasmid cells, its integration into their nuclear DNA. We show here group aldoses (L-arabinose,...
Most vir genes of Agrobacterium, which are required for tumorigenicity the bacterium, expressed in response to plant phenolics. The induction is markedly enhanced by specific monosaccharides. Signals generated both types compound transduced into Agrobacterium cells via functions VirA membrane-bound sensor protein. A putative sugar-binding protein, known as ChvE, also at a step enhancement To investigate signal pathway sugars, we first isolated virA mutant with base substitution mutation that...
<title>Abstract</title> Background Methylation capture sequencing (MC-seq), which relies on next-generation technology, offers advantages over the widely used array-based approach that Illumina Inc. developed regarding both resolution and comprehensiveness for detecting DNA methylation changes across genomes. In present study, MC-seq was employed first time to identify markers Alzheimer’s disease (AD). Results We compared in blood of 12 AD patients with brain amyloidosis cognitively normal...
Although dedifferentiation, transformation of differentiated cells into progenitor cells, is a critical step in the regeneration amphibians and fish, molecular mechanisms underlying this process, including epigenetic changes, remain unclear. Dot blot assays immunohistochemical analyses revealed that, during zebrafish fin, levels 5-methylcytosine (5mC) 5-hydroxymethylcytosine (5hmC) are transiently reduced blastema adjacent to amputation plane at 30 h post-amputation (hpa), level 5mC, but not...
The aim of the present study was to (1) investigate relationship between late-onset Alzheimer’s disease (AD) and DNA methylation levels in six top seven AD-associated genes identified through a meta-analysis recent genome wide association studies, APOE, BIN1, PICALM, CR1, CLU, ABCA7, blood, (2) examine its applicability diagnosis AD. We examined differences at CpG island shores using Sanger sequencing, one two groups 48 AD patients elderly controls used for test or replication analysis....
The zebrafish no tail gene (ntl) is indispensable for and notochord development. We have shown previously that ntl de novo methylated during early embryogenesis. To find the methylates understand meaning of this methylation, we cloned seven genes encode conserved catalytic domain methyltransferases. found injection antisense morpholino oligonucleotides against one them, termed dnmt7, into eggs significantly reduced level although apparent phenotype was induced by injection. Inhibition Dnmt7...
The mechanisms responsible for active DNA demethylation remain elusive in Metazoa. A previous study that utilized zebrafish embryos provided a potent mechanism which three proteins, AID, MBD4, and GADD45 are involved. We recently found age-dependent hypomethylation zebrafish, it prompted us to examine if AID MBD4 could be involved the phenomenon. Unexpectedly, however, we most of findings were not reproducible. First, injection methylated fragment into eggs did affect either methylation...
Glial activation is central to the pathogenesis of Alzheimer's disease (AD). However, researchers have not demonstrated its relationship longitudinal cognitive deterioration. We aimed compare prognostic effects baseline positron emission tomography (PET) imaging glial and amyloid/tau pathology on successive annual decline in patients with AD.We selected 17 diagnosed mild impairment or AD. assessed changes global cognition memory. Furthermore, we predictive amyloid tau indicated by...
Neuroinflammation is a well-known feature of Alzheimer's disease (AD), and blood-based test for estimating the levels neuroinflammation would be expected. In this study, we examined validated model using biomarkers to predict level glial activation due neuroinflammation, as estimated by 11C-DPA-713 positron emission tomography (PET) imaging.We included 15 patients with AD 10 cognitively normal (CN) subjects. Stepwise backward deletion multiple regression analysis was used determine...
Positron emission tomography, which assesses the binding of translocator protein radiotracers,
Abstract The zebrafish no tail gene ( ntl ) is indispensable for the formation of notochord and structure. Here we showed that de novo DNA methylation occurred at CpG island . started segmentation stage continued after larval stage. However, it predominantly between 14 48 h postfertilization, which overlaps period in expression disappears tailbud. This inverse correlation, together with methylation‐associated an inaccessible chromatin structure region, suggested involvement repression. Since...
Abstract The aim of the present study was to (1) investigate relationship between late onset AD and DNA methylation levels in top six Alzheimer’s disease (AD)-related genes blood (2) examine its applicability diagnosis AD. We examined differences at CpG island shores using Sanger sequencing, one two groups 48 patients elderly controls used for a test or replication analysis. found that three out genes, CR1, CLU , PICALM were lower subjects. combination APOE genotype classified with AUC=0.84...
The evaluation of 11 C-DPA-713 binding using positron emission tomography for quantifying the translocator protein can be a sensitive approach in determining level glial activation induced by neuroinflammation. Herein, we aimed to investigate relationship between regional C-DPA713-binding potential (BPND ) and neuropsychiatric symptoms (NPS) amyloid-positive Alzheimer's disease (AD) patients.Fifteen AD patients were enrolled this study. Correlations evaluated C-DPA713-BPND Neuropsychiatric...
DNA methylation is an epigenetic regulator mediated by methyltransferases (Dnmts). The involved in control of gene expression vertebrates. It has been reported that there are mainly two types de novo Dnmts, Dnmt3a and Dnmt3b, mammals. These Dnmts function the distinct or overlapping genomic regions. zebrafish homologs mammalian Dnmt3aa Dnmt3ab. We generated a maternal-zygotic dnmt3aa deficient mutant (MZdnmt3aa) to identify specific target regions for genome their developmental process....